FastQCFastQC Report
Tue 10 May 2022
EGAF00003639376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173360
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103845.98984771573604No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58583.379095523765575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31271.8037609598523303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT27821.604753114905399No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21381.2332718043377942Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21011.2119289340101524Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19751.1392478080295338Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15850.9142824180895247No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13790.7954545454545454Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13110.7562298107983387Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.5727964928472543No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT8400.4845408398707891No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA7550.43550992155053064Clontech Universal Primer Mix Long (96% over 26bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT7290.4205122288878634No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC7250.4182048915551454No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7070.40782187355791416Clontech Universal Primer Mix Long (96% over 26bp)
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA7070.40782187355791416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.37494231656668203No Hit
GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC6440.37148131056760497No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6360.3668666359021689No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5760.33225657591139823Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5580.32187355791416705Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5520.31841255191509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG5240.30226119058606365No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5020.2895708352561145Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4840.27918781725888325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTG4830.27861098292570374No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4740.27341947392708815Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT4680.26995846792801104No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4660.26880479926165207No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4630.26707429626211354Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.26130595293031844No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT4430.2555376095985233No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4320.24919243193354867Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT4320.24919243193354867No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4260.24573142593447161Clontech Universal Primer Mix Long (96% over 26bp)
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC4130.23823257960313798No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT4110.23707891093677894No Hit
CATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG3910.22554222427318876No Hit
ACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTA3810.21977388094139363No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA3460.19958467928011075No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG3410.1967005076142132No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3370.19439317028149516Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3330.19208583294877712Clontech Universal Primer Mix Long (96% over 26bp)
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT3170.18285648361790494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3150.18170281495154592No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG3070.1770881402861098No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3010.17362713428703275Clontech SMART CDS Primer II A (100% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2980.17189663128749422Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2940.1695892939547762Clontech Universal Primer Mix Long (96% over 26bp)
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG2910.16785879095523765No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC2890.16670512228887863No Hit
ACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC2790.16093677895708353No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2730.15747577295800647Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC2670.15401476695892938Clontech Universal Primer Mix Long (96% over 26bp)
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG2640.15228426395939085No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT2640.15228426395939085No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG2620.15113059529303183No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2590.1494000922934933No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2590.1494000922934933Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT2570.1482464236271343No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2480.1430549146285187Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC2450.14132441162898016No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2360.13613290263036457No Hit
GGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTC2340.13497923396400552No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG2330.13440239963082604No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.132095062298108No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT2250.12978772496538993Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG2210.1274803876326719No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2170.12517305029995385Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2160.12459621596677434Clontech Universal Primer Mix Long (96% over 26bp)
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT2150.12401938163359484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2150.12401938163359484No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2070.11940470696815875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.11882787263497922Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2020.1165205353022612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT1980.11421319796954316No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.1119058606368251Clontech Universal Primer Mix Long (95% over 21bp)
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA1890.10902168897092754No Hit
GTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGC1860.10729118597138901No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1830.10556068297185048Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA1790.10325334563913244No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC1750.10094600830641438No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)