FastQCFastQC Report
Tue 10 May 2022
EGAF00003639419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476736
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA299646.2852396294804675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATG147593.0958434017988994No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG135402.840146328366224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132732.784140488656196Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG109792.302951738488388Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCG99322.083333333333333No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC93451.9602043898509867Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG86561.8156799570412137Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76631.607388575647738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCGTC56861.1926936501543832No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46340.9720264465028864Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41960.8801516982145254Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCGCGTATG37750.7918428648140691No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG37680.7903745469190495Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT35850.7519885219492549Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA31680.6645187273459524Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30850.6471086723050073Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCGTCTTC30520.640186602228487RNA PCR Primer, Index 21 (95% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC29910.6273912605718889Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27620.5793562894348234Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA25970.5447459390522218Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG25440.5336286749899315Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCGCGTATGCCG24290.5095063095717546No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA23780.4988085649080413Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23460.49209625453080946Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21030.4411246476037052Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20090.42140723587058665Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG19960.4186803597798362Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19670.4125973285004698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT19620.4115485300040274Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC19560.4102899718082964Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA18520.38847496308229296Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC16690.3500889381124983Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCGTCT16680.34987917841320987No Hit
CTTATACACATCTCCGAGCCCACGCGACCTCGCCTATGCGATCTCGTATG15900.3335179218687072No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA15790.33121056517653374Clontech Universal Primer Mix Long (95% over 21bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15750.33037152637937983Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA15600.32722513089005234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15260.3200933011142435Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC14730.3089760370519533Clontech Universal Primer Mix Long (96% over 25bp)
TCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCGTCTTCTG14420.30247348637400995RNA PCR Primer, Index 33 (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT13920.2919855014095852Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT13460.28233655524231444Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.2695412135857162Clontech Universal Primer Mix Long (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCGCGTATGCCGTC12370.2594727480198684No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12290.2577946704255605Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA12270.2573751510269835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11680.24499932876896227Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10740.22528191703584374Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.21689152906430392No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10260.215213451469996Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9910.20787186199489865Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9780.2051449859041482No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT9330.19570579943616592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGC8820.1850080547724527No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA8600.18039334138810578Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.17682742650020136No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT8360.1753591086051819Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT8310.17431031010873943Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA8290.17389079071016245Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC8160.171163914619412Clontech Universal Primer Mix Long (96% over 25bp)
ACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCCGTCTT8100.16990535642368104RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGCGACCTCGCCTATGCGATCGCGTATG8070.16927607732581554No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT7770.1629832863471607Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGCGACCTCGCCTATGCGATCTCGTATGCCG7560.15857833266210228No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6950.1457829910055041Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6720.1409585179218687Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGCGACCTCGCCTATGCGATCTCGTATGCCGTC6660.13969995972613775No Hit
TATACACATCTCCGAGCCCACGAGACCTCGCCTATGCGATCTCGTATGCC6510.1365535642368103No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT6480.1359242851389448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA6370.1336169284467714No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA6320.1325681299503289Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6260.13130957175459793Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6140.128792455363136Clontech SMART CDS Primer II A (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC6090.12774365686669353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5910.1239679822795006Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT5910.1239679822795006Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5790.12145086588803866Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.11935326889515371No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG5630.11809471069942273Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA5220.10949456302859445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA5130.107606725734998Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTCCGAGCCCACGAGACCTCGCCTATGCGATCGCGTATGCCGTCTTC5040.10571888844140152RNA PCR Primer, Index 33 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)