FastQCFastQC Report
Tue 10 May 2022
EGAF00003639757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003639757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116640
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31842.729766803840878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCTCGTATG16841.443758573388203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16561.4197530864197532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15331.3143004115226338Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11681.0013717421124828Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11450.9816529492455418Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11260.9653635116598079No Hit
ATACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCTCGTATGCCG10470.8976337448559671No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCGCGTATG9090.779320987654321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.7690329218106996No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8100.6944444444444444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCTCGTATGCCGTC7060.605281207133059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.6001371742112482No Hit
ATACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCGCGTATGCCG6740.5778463648834019No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5990.5135459533607681Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT5020.43038408779149523Clontech SMART CDS Primer II A (100% over 26bp)
ACACATCTCCGAGCCCACGAGACCAGGACTATTTCATCGCGTATGCCGTC4860.4166666666666667No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA4780.4098079561042524No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4550.3900891632373114Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4510.3866598079561042Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4300.3686556927297668No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3980.34122085048010975Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3940.3377914951989026Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.33521947873799723No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT3430.29406721536351166Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA3210.2752057613168724No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3190.2734910836762689Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2780.23834019204389575Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2690.23062414266117967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2620.22462277091906724Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA2580.22119341563786007No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2540.21776406035665294Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2450.2100480109739369No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2440.20919067215363513Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2360.20233196159122083Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT2330.1997599451303155Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2250.19290123456790123No Hit
CATCTCCGAGCCCACGAGACCAGGACTATTTCATCTCGTATGCCGTCTTC2160.1851851851851852RNA PCR Primer, Index 35 (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2150.1843278463648834Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2110.18089849108367625No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.17832647462277093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.17146776406035666No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1980.1697530864197531Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1970.1688957475994513Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1950.16718106995884774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1920.1646090534979424Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1900.16289437585733882No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1740.1491769547325103No Hit
CACATCTCCGAGCCCACGAGACCAGGACTATTTCATCTCGTATGCCGTCT1740.1491769547325103RNA PCR Primer, Index 35 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1730.14831961591220852Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1690.14489026063100136No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1660.142318244170096Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1650.14146090534979425Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1640.14060356652949246No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1630.13974622770919065Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.13631687242798354No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1580.13545953360768176No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1560.1337448559670782No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.13117283950617284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1530.13117283950617284No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1520.13031550068587106No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1520.13031550068587106Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1510.12945816186556927No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1490.1277434842249657No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT1460.12517146776406035Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCAGGACTATTTCATCGCGTATGCCGTCT1410.12088477366255143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1390.11917009602194788No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1310.11231138545953362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1300.11145404663923182No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1300.11145404663923182Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1280.10973936899862827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1270.10888203017832647No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1260.10802469135802469Clontech Universal Primer Mix Long (95% over 22bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1250.10716735253772292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1230.10545267489711935No Hit
CATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.10373799725651577No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1200.102880658436214Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1180.10116598079561041No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)