FastQCFastQC Report
Tue 10 May 2022
EGAF00003640090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255288
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16770.6569051424273762No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT11590.45399705430729215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.2683244022437404No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG5530.21661809407414373No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG4720.18488922315189119No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC3990.15629406787628092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.15041835103882673No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG3780.14806806430384506No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA3660.14336749083388173No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA3250.12730719814484034No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3160.12378176804236783Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG3150.12339005358653755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.12103976685155589No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3080.12064805239572561No Hit
GGACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGG3050.11947290902823479No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG2930.11477233555827143No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA2920.11438062110244117No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA2920.11438062110244117No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC2900.11359719219078061No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA2880.11281376327912004No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC2680.10497947416251449No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG2670.10458775970668421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)