FastQCFastQC Report
Wed 11 May 2022
EGAF00003640137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5067973
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT179250.35369170277742207No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA130610.25771644797634086No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT117470.23178892231667372No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT110600.21823320684620853No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG107980.2130634871180253No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG107070.21126789744144256No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG105470.20811081669140702No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG103270.2037698306601081No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG97210.19181238731934838No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC96000.189424845002134No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG84450.16663466833781473No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG79600.15706476731426944No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA72240.14254219586410583No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC69740.13760925719217526No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG69400.1369383775327927No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT68470.13510332434683453No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT65440.12912460267645468No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG63050.12440871330608905No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG62520.12336293030763976No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT60420.11921926182321808No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA59850.11809455180601791No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA58740.11590432703568072No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC58600.11562808247005261No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG54530.10759725831214965No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT54110.10676852461526533No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC53600.10576220512619147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)