FastQCFastQC Report
Tue 10 May 2022
EGAF00003640452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728552
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT78140.2863790024892324No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA61970.2271168004128197No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC55410.203074744406557No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG53690.19677103460003695No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG43490.15938856946834803No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG40020.14667120142844997No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG40000.14659790247721136No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT35480.13003233949728646No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA34820.1276134741064125No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC34070.12486476343496478No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG31660.11603223981071278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.11178090063887365No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG28930.1060269329666431No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC28660.10503739712492194No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC28580.1047442013199675No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG28260.10357141810014982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)