FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003640687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCTCGTATGCCG53.4722222222222223No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.3888888888888888Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCGCGTATGCCG21.3888888888888888No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.3888888888888888Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCGCGTATGCCGTC21.3888888888888888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.3888888888888888No Hit
ACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCTCGTATGCCGTC21.3888888888888888Illumina PCR Primer Index 9 (95% over 21bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT21.3888888888888888No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21.3888888888888888No Hit
TTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGAATGGAATGGGGTGGATTGGAATCAATCTGAGTGGAATGGAAAG10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAAGCC10.6944444444444444No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAAAAAACCAG10.6944444444444444No Hit
ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTAT10.6944444444444444No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT10.6944444444444444No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTCACAAAAAAACAA10.6944444444444444No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGC10.6944444444444444No Hit
CAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTC10.6944444444444444No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGACAAACTTCA10.6944444444444444No Hit
GTACAGGGCGATAAAGCCAGACCTTGTCTCACAAAAAAAAGGGGGTGGGG10.6944444444444444No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTCACAACAACCCCCGGG10.6944444444444444No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
CATCTGGTCAGGGTACCGCCGCCCGGGAGGTGGATCGGCGAGCCCACCGC10.6944444444444444No Hit
GTGATACACGTTTCTGGAAACCGCCTCGTCATGCGACTGTTCCCCGGGGT10.6944444444444444No Hit
GCAGAGTACATGGGAATGGCTCACTCATGCCTGTAATCCCAGCACTCTGG10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAACA10.6944444444444444No Hit
GGTAAAAAGCCCAATTGTGTGGTGATGAATTAGAAAACTTCATGAACATT10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTGAAACCACACCAAG10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTATAAAAAAAACCCAGC10.6944444444444444No Hit
GATTACAGGCGTGAGCCACCGTGCCCGGCCTGTCTCTTTCTTTTCTCTGT10.6944444444444444No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAG10.6944444444444444No Hit
GCCTATGGCGAGGTGTTCGCCCGAAATGTCAAGGCGTCGGGTGTCATCCC10.6944444444444444No Hit
CCCTCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGGTCTGAT10.6944444444444444No Hit
TATCAGTTCTTTCTTTCATTGACCATGCCTTTTGTGCTGCTGTATCTAAA10.6944444444444444No Hit
CGTTAATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATAACAATGATTCC10.6944444444444444No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT10.6944444444444444No Hit
CCTGTGGTGATCCGCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGAC10.6944444444444444No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.6944444444444444Clontech SMART CDS Primer II A (96% over 26bp)
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC10.6944444444444444No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA10.6944444444444444No Hit
GGTTCAAGCAATTCTTTTGCCTCAGCCTCCCGATTAGCTGGGATTGCAGG10.6944444444444444No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGGAGTACATGGGAAGCAGTGGTATCAAC10.6944444444444444Clontech Universal Primer Mix Long (95% over 23bp)
GGAAGCAGTGGTATCAACGCAAAGTACATGGGAAGCAGTGGTATCAACGC10.6944444444444444Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGCCGGATTGCCTGAA10.6944444444444444No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGACTCACACACAGTC10.6944444444444444No Hit
CTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTGAC10.6944444444444444No Hit
GAGTACATGGGACTTGGGAGGCTGAGGCAGGAGAATCTCGAACCCAGGAG10.6944444444444444No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAATC10.6944444444444444Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAAAAACCGACCCCA10.6944444444444444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAACA10.6944444444444444No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC10.6944444444444444No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACA10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAATATAAAAATAACCGAGC10.6944444444444444No Hit
GTTGTGTCCAGTCCCTATCGGACTGGCCGCGCTGGTGCCACACGACTACA10.6944444444444444No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATCT10.6944444444444444No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.6944444444444444Clontech SMART CDS Primer II A (100% over 26bp)
GGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC10.6944444444444444No Hit
ACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCTCGTATTCCGTC10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAAACCGAGCCC10.6944444444444444No Hit
GGAGTAGGCTCTCCAGCAGCTGGCGGGGGGTGACTGCTGAGGAAGTGATC10.6944444444444444No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT10.6944444444444444No Hit
GGGATACAGTACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACT10.6944444444444444No Hit
GGCTAACACGATGAAACCCCGTCTCTATGTGCGTGGTGGCTGGCACCTGT10.6944444444444444No Hit
TTATACACATCTCCGAGCCCACGAGACTTGTGGGCTCAGATCTCGTATGC10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACACAACACCCAGCCC10.6944444444444444No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGG10.6944444444444444No Hit
ACTCTACGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.6944444444444444Clontech Universal Primer Mix Long (95% over 23bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC10.6944444444444444No Hit
CTGTCTCTGTCTGTGTCTCCCTCTCTGCTTGTCTCTGCCCCTGTCTCTCT10.6944444444444444No Hit
GCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGC10.6944444444444444No Hit
GCATCCTTATACAGTACGCAGCGACCGTAACGCTGTATGAGATCGCCGCA10.6944444444444444No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.6944444444444444Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.6944444444444444Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG10.6944444444444444No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA10.6944444444444444No Hit
ATCTCGGTCGTCGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT10.6944444444444444No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.6944444444444444Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCCCAGGCCGAGCGTAAAATCGAACGGCCCCCAGGCTTTGCTGGCAACA10.6944444444444444No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTTCGTTGAT10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
TTTATTAGAGATGGGGTTTCACTATGTTGGCCACACTGGTCTCGAACTCC10.6944444444444444No Hit
CCCAAAGTGCTGGGATACCAGGCGTGGGCCACTGCACCTGGCCAATTACG10.6944444444444444No Hit
GTACATGGGGTTGAGAGGGGAAGGAGAGGAGAGGGAGGAAGAGAGGCATG10.6944444444444444No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCACTAAAAAAA10.6944444444444444No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT10.6944444444444444No Hit
GTTCCTCTTACTTCTCCTTCTTTAGTTGTTCCTCCTCTTCCTTCTCCTTC10.6944444444444444No Hit
CGGAGTAGCCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGGGCTG10.6944444444444444No Hit
GCGTTGATACCACTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGCATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.6944444444444444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTCCTTCTTCTTAGTTCTTCTTAGTTCTTCTTCTTCTTCTTCTTTCTC10.6944444444444444No Hit
ACTTTGGGAGGCCGACGCGGGCGGATCACGAGGTTAGGAGATTGAGACCA10.6944444444444444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGTGCTTGTTCC10.6944444444444444No Hit
CATGGGAGCCCACCAAAAGAAGAAATGGGAAATGAACACATCAGTGTCCA10.6944444444444444No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCAT10.6944444444444444No Hit
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAG10.6944444444444444No Hit
GGCTGGTCTCAAACTACCGACTTCAGGTGATCCGCTCGCCTTGGCCTCCC10.6944444444444444No Hit
ACCCAACAACGCATAAGCGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGC10.6944444444444444No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAAGTTTCGCTT10.6944444444444444No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC10.6944444444444444No Hit
ACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCG10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTAAAAAACC10.6944444444444444No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGCA10.6944444444444444No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
ATACAGGACTCTTTCGAGGCCCTGTACTTGGAATGAGTCCACTTTAAATC10.6944444444444444No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC10.6944444444444444No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC10.6944444444444444No Hit
GTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCT10.6944444444444444No Hit
GAGACAGAGAGGGAGACAGAGAGACAGGGGCAGAGACAAGCAGAGAGGGA10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTACACATATCCGACCCCACGA10.6944444444444444No Hit
AAATACACTCGCTGTTGAGATACTAAAATTATCTGTGGATTGCCGGCATT10.6944444444444444No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTGAAAAACAACCCCGGT10.6944444444444444No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTCTGT10.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG10.6944444444444444No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACACAGCCATCC10.6944444444444444No Hit
GTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGAC10.6944444444444444No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT10.6944444444444444No Hit
GTGTCCCCTCTCTTTCCAACTATATGCTTATAGAGCAAGCTGGGGGCCCT10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAGTACTCTGCGTTTATACCACTGCTTCCCATG10.6944444444444444Clontech SMART CDS Primer II A (96% over 26bp)
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC10.6944444444444444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)