FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003640769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA671.7175083311971289Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT591.512432709561651Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG310.7946680338374775Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG190.4870546013842604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG180.4614201486798257Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC180.4614201486798257Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.4614201486798257No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC170.43578569597539096Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC170.43578569597539096Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC170.43578569597539096No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG160.41015124327095615No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC140.3588823378620867Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC140.3588823378620867No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG130.33324788515765186Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT120.30761343245321715No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT110.2819789797487824No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG110.2819789797487824No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA110.2819789797487824No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT100.25634452704434757Clontech Universal Primer Mix Long (96% over 26bp)
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG100.25634452704434757No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG100.25634452704434757Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC90.23071007433991286Clontech Universal Primer Mix Long (96% over 26bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA90.23071007433991286No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT90.23071007433991286No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG90.23071007433991286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80.20507562163547807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT80.20507562163547807No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.20507562163547807Clontech Universal Primer Mix Long (100% over 23bp)
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG80.20507562163547807No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT80.20507562163547807No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT80.20507562163547807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.20507562163547807No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70.17944116893104334Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA70.17944116893104334No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT70.17944116893104334No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG70.17944116893104334No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC70.17944116893104334No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC70.17944116893104334No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT70.17944116893104334No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC70.17944116893104334No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70.17944116893104334Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.17944116893104334Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG70.17944116893104334No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC70.17944116893104334Clontech Universal Primer Mix Long (96% over 26bp)
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT70.17944116893104334No Hit
GCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCA70.17944116893104334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.17944116893104334No Hit
ACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAA60.15380671622660858No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG60.15380671622660858No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG60.15380671622660858No Hit
GCCTAACTGTAATTGATCCACACTGACGAATCATGTACTCACTCGATCTG60.15380671622660858No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG60.15380671622660858No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG60.15380671622660858No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG60.15380671622660858No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.15380671622660858No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT60.15380671622660858No Hit
GCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGA60.15380671622660858No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT60.15380671622660858Clontech SMART CDS Primer II A (100% over 26bp)
GTCCAGCGGCAGGCGAGAGGTGCTGCGAATGCCGTTCGAGTCGAAGTCGC60.15380671622660858No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA60.15380671622660858Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.15380671622660858Clontech Universal Primer Mix Long (96% over 26bp)
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG60.15380671622660858No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG60.15380671622660858No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC60.15380671622660858No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT60.15380671622660858No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA50.12817226352217378No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA50.12817226352217378No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC50.12817226352217378No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG50.12817226352217378No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA50.12817226352217378No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG50.12817226352217378No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC50.12817226352217378No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.12817226352217378Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC50.12817226352217378No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT50.12817226352217378No Hit
CTCATACAGTCACTCTACACTGAGGAAAATGACTAGAAAGAAGAACTCAC50.12817226352217378No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC50.12817226352217378Clontech Universal Primer Mix Long (96% over 25bp)
GGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA50.12817226352217378No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC50.12817226352217378No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT50.12817226352217378No Hit
AAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTT50.12817226352217378No Hit
CTTGTTGGAATGCTCCCTTATGCAGGTTTAAAGTTCTACGTCTATGAAGA50.12817226352217378No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC50.12817226352217378No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG50.12817226352217378No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG50.12817226352217378No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT50.12817226352217378No Hit
TCTCATTGCTGCAGCGGATGGAGTGCCGACTCGAAGAACTCGGTCAGCGC50.12817226352217378No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG50.12817226352217378Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA50.12817226352217378No Hit
TCTATTCCTATTCGGCGATCGCCAACGAGAAACTCAGCGGCGCAGGCCTG50.12817226352217378No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG50.12817226352217378No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT50.12817226352217378No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.12817226352217378Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.12817226352217378Clontech Universal Primer Mix Long (96% over 26bp)
CCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGC50.12817226352217378No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG40.10253781081773904No Hit
TTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCA40.10253781081773904No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT40.10253781081773904No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10253781081773904No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT40.10253781081773904No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10253781081773904No Hit
GCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTAT40.10253781081773904No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC40.10253781081773904No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT40.10253781081773904No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG40.10253781081773904No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT40.10253781081773904No Hit
GTTGTAGGTCGGAGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA40.10253781081773904No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT40.10253781081773904No Hit
CACTAGAGCGACACCAACATCGTTACGCTTACACACCGGACGCTTGGATC40.10253781081773904No Hit
GCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACC40.10253781081773904No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT40.10253781081773904No Hit
ATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATG40.10253781081773904No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT40.10253781081773904Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAA40.10253781081773904No Hit
CAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCG40.10253781081773904No Hit
GCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAAT40.10253781081773904No Hit
CGGTGGTGGGCTCGGGCAACCGCGCGGGCGTGAAGGCCGCAGCCATCGAG40.10253781081773904No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.10253781081773904Clontech Universal Primer Mix Long (96% over 26bp)
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG40.10253781081773904No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG40.10253781081773904No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.10253781081773904Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10253781081773904No Hit
GCCCCGGGTTCGTCCCCTATAAGGTCCTAGTACCTTTCGAGCCCCGCGCG40.10253781081773904No Hit
AAATGAACCTGCCGGCTGATCGTTTTTAGGATATTGTGAGTAATATAGAT40.10253781081773904No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT40.10253781081773904No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA40.10253781081773904No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG40.10253781081773904No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG40.10253781081773904No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG40.10253781081773904No Hit
CGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTC40.10253781081773904No Hit
CTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGA40.10253781081773904No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC40.10253781081773904No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA40.10253781081773904No Hit
GCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAA40.10253781081773904No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT40.10253781081773904No Hit
ATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTG40.10253781081773904No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC40.10253781081773904No Hit
CATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG40.10253781081773904No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT40.10253781081773904No Hit
GTGCCCTTGCGCGTGCCGCCGTCGCCGTCGCCCGCCTCGGTCTCGATGGC40.10253781081773904No Hit
TCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGC40.10253781081773904No Hit
CTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGC40.10253781081773904No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA40.10253781081773904No Hit
ACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCA40.10253781081773904No Hit
AGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAG40.10253781081773904No Hit
AGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTG40.10253781081773904No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT40.10253781081773904No Hit
GAGTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC40.10253781081773904No Hit
GAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAA40.10253781081773904No Hit
GAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGG40.10253781081773904No Hit
TCGTCATGTCGTCGTTGTTTCTGCTCAGGAGTCCGGACTTCACACCGGAA40.10253781081773904No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA40.10253781081773904No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA40.10253781081773904No Hit
TAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATT40.10253781081773904No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT40.10253781081773904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)