FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003640925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACCCATCATAATCGGAGGCTTTGGCACCTGACTAGTTCCCCTAAT46.153846153846154No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT34.615384615384616No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA34.615384615384616No Hit
GGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA34.615384615384616No Hit
GCAGTGTGTGGAGATGTACACAGATGGGCAGTGGAATGACAGGAACTGCC23.076923076923077No Hit
AAAGAGGACACAAAGAAATGGAAGGACATTCCATGCTCATGGATAGGAAG23.076923076923077No Hit
TCACAAGACAGCTGTCTCAGGCTTTTCAAGCTGTGAGAGACACATCAGAG23.076923076923077No Hit
CAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCC23.076923076923077No Hit
TTCTGAATCTCATCCTGCAGGCGGCCAATAGTGTCTTGGTAGTTAGCAGC23.076923076923077No Hit
GGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGC11.5384615384615385No Hit
GTGAAGTAGAGCCCGGGGTAGGCGGTGAAGTAGGTCTGGAAGAAGCGCTC11.5384615384615385No Hit
GCTGGCATGGTTTCTTAAAACCACCTCTTCCTATTTTCAGTCTGTGGTTT11.5384615384615385No Hit
CCAGATGTCCCTCCCAGTTGGGAAGACTAAACTGGTTTGGCCAATATCTC11.5384615384615385No Hit
GGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACC11.5384615384615385No Hit
GGAAAGAACTTCCCCTTCCCTGTCTCCCATCCCTCTGTGGCAGGCGCCTC11.5384615384615385No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG11.5384615384615385No Hit
CTCCCAACGCTACCATTGTGGCGGAACGTTCGAGGCGGTTTTCTAGGGCG11.5384615384615385No Hit
AAAAAGTAGAGAAATCCGAGAAGTAAAATCAATCTCCCAATCCCTTGGAA11.5384615384615385No Hit
TGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG11.5384615384615385No Hit
ACATAGTAGGGAGTGTTGTTCATCATGATTGAAGCCTTACTAAATTTTGA11.5384615384615385No Hit
TTCTTCCACTGTCGGAGATAACTATTTCACATCTTCTTCTTTATGTTCAA11.5384615384615385No Hit
AAATAAAAAAATAAAAAATAAAACAATGATATATTATTAAGACAAAAAAA11.5384615384615385No Hit
GAGCATATATGTATGTGTGTGCGCATGTAAAAGTATGAATCTCTGTCTGT11.5384615384615385No Hit
GGACAAGGTGCTGGTGGTGCAGGCCAAACGCGGCGGCACCGGGCCGCAGC11.5384615384615385No Hit
GGACCCGAGTGGACAGAGGCACGTGGTTGTTTGCAGTGGCAAAGAAGACA11.5384615384615385No Hit
ATCCGATGCCGAGGAGCGCGATGCCGGCGCCCTGGATCACCAGCGCCGTG11.5384615384615385No Hit
GCCTTGGAGTCAGAAGATCTGAGTTTGAATCTTAGTTTCTCAATACTCTG11.5384615384615385No Hit
AATCGAGACCATTCGATGATTCCATTCAATTCCATTCAATAATGATCCCT11.5384615384615385No Hit
CTGTGAATTGATCGCACACATCACAAAGTAGATTCTCAGAATGATTCTGT11.5384615384615385No Hit
AGTGACTACAGCAGTTGTTGACAGATTTTTTTCTTGAGAGACCATATAGT11.5384615384615385No Hit
GAGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCCATACATTGGG11.5384615384615385No Hit
TCTTCCTCCCGTGAGGCTGGCCGAGCTCCACGCTGATCTGAAGATACAGG11.5384615384615385No Hit
ATTCTGTTGAGATGGCTCTTCCCTCTGAACGTGCTCTCCTACTGACCACC11.5384615384615385No Hit
GTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATA11.5384615384615385No Hit
GGTGATTGGCAGATGGAGTCTCTCATTCGTTACATCAAGGTGATCGGTGG11.5384615384615385No Hit
ACACGCTGCTGGGCCGCATGCTGCCCCAGCTGGTCTGCCGCCTCGTCCTC11.5384615384615385No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA11.5384615384615385No Hit
TTTTAGTAGAGATGGGGGTTTCATCATGTTGGCCATAATGGTCTTGATCT11.5384615384615385No Hit
GAGGGATAGCATTAGGAGAAATACCTAATGTTGAATGATGAGTTAATGGG11.5384615384615385No Hit
TTGCAGGCCGCCGCCGCGGCGCTCGATGCCGCTCGCGACGAACTGGTGCG11.5384615384615385No Hit
GATGATCATCGAGCCCACCAGCCCTTGCCATCTCCCAGACCCTCTGCAGG11.5384615384615385No Hit
GACCCAGGTGCTCGCCGAAGCCATCCGGGTGCGCCCCAAGCCCACCGGGC11.5384615384615385No Hit
ACTCTATGAGTTGAATGCAGACATCAGAAAGAAATTTCTGAGAATGCTGC11.5384615384615385No Hit
GTCTATGGGACCAGTGGCTTGGATTCTGCCACACCCATAAATCCTTGTGT11.5384615384615385No Hit
GATGATCATCGAGCCCACCAGCCCTTGCCTTCTCCCAGACCCTCTGCAGG11.5384615384615385No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG11.5384615384615385No Hit
GATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGG11.5384615384615385No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT11.5384615384615385No Hit
TTTCCCTATGATAAACAAACCTCTGTCTCTGTTTTCTTTTTCTAGCAAAT11.5384615384615385No Hit
CCCTTAAAGGAACCAATGAGTCCCTGGAACGCCAGATGCGTGAAATGGAA11.5384615384615385No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)