FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003640926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003640926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGT46.153846153846154No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA34.615384615384616No Hit
GCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTA34.615384615384616No Hit
CCACCACCCCATCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGG23.076923076923077No Hit
ACCTCATGGAGAGCCTGACCAGGCCTGCCACAGAGACCTCAGAGTGCCAG23.076923076923077No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT23.076923076923077No Hit
GTATCAACGCAGAGTACATGGGTAGGCAAAGCAGGAGTCCACTGAGTACC23.076923076923077No Hit
CACTATCAATGAGTGGAAACGCAGTCATGCCAGTCTCTGACGTAAAGATG23.076923076923077No Hit
GCTTAGGATTGCGTTGGCACTGAGGGCTCTTTTTTTGATTCCACATGAAC23.076923076923077No Hit
GGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCTGGAAAGGCGG23.076923076923077No Hit
CTGCTAGACAGAATAAATCTTAATAACTTCCTTGGGTTGTGTGTATTCAA11.5384615384615385No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCA11.5384615384615385No Hit
GAAGACATGCTCTACAGTGCTCAGTCTTTGAGGTCACGAGAAGAGAATGC11.5384615384615385No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT11.5384615384615385No Hit
ACCACATCCAGCCTGAACGACAGAGCGAGACTCCTTCCCAAAACAAAACA11.5384615384615385No Hit
GGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAA11.5384615384615385No Hit
TGTCCAGTCTCTCAGGAGTTAGCAGGCTTACACACGAGGAGACGAAGGCT11.5384615384615385No Hit
GCCCGACTTCAGGCACAGGCTGGCCGCGTCGATGGTCACGTTGGGCCGCG11.5384615384615385No Hit
ATAAATGCCAATGTAGGAATTACCGTGACAATAAACATTCCAAATGAAAT11.5384615384615385No Hit
TCTTCTTACGGGAGGCACTCATGTCCAAGCAGCGCACAGCTGTGGCCTGC11.5384615384615385No Hit
AAACGGGCGGCATCCTCATTTCGCCCCTGCCCGGCGCAACCGACCTCAAG11.5384615384615385No Hit
GGGGTCATCCAGCCCGTCTTCCTGGGTGCCACTGAGGGAGTTGCTGGTCA11.5384615384615385No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.5384615384615385No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC11.5384615384615385No Hit
GAACAGGGTATAAGGGTAAATATACAGATAATTTGCAAGTGTGGAATTGC11.5384615384615385No Hit
GAACAGAAGAAAGCAAAGGACTTATGGAAGGCACGGACCCAACCACCCCA11.5384615384615385No Hit
CACAGAATGCGAGCGATTGGCGGTGGCTCAACCTTTCGCCTCGTTCTCCA11.5384615384615385No Hit
CACCATCGAATTGAAACGAATGGAATTACCATGAAATTGAAATGGATGGA11.5384615384615385No Hit
GCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCA11.5384615384615385No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGAGGCGGCGTTATT11.5384615384615385No Hit
GGTCTGACTGCTCATCTTGTAGCGGCTTCCATCTCACTGGAGGTTCCACT11.5384615384615385No Hit
TCTTAGAGCACGTTTGAAACACTCTTTTTGTAGTGTCTGGAAGTGGACAT11.5384615384615385No Hit
GTTCAGTATTTAACCTTCTAATTATGTATGTATATAGTTTAGAATTCAGG11.5384615384615385No Hit
AAGTTTGGAAGAGGCAGAGAAATCCTGCTCTCCTCGCCTTCCAGCAGCTT11.5384615384615385No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG11.5384615384615385No Hit
GCTTTTCAATCAGGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCC11.5384615384615385No Hit
GCCTGTGGTATGGCCTGCTCGCTGCTGTTCCCGGCCTGGGTGGTCACGGA11.5384615384615385No Hit
TTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCAC11.5384615384615385No Hit
GGGTGAAACCCCATCCCTATAAAAATACAAAATTAGGCCGGGTGCGGTGG11.5384615384615385No Hit
GGCGACGCGACAGGGTTGGGGCGCTGCGGCCGTGGGCGCGTTCGAGGCGC11.5384615384615385No Hit
GCCTCTTCAGGCAACAAAGTTTCAGAGATACTTGGGGGCGTAAAATTCTT11.5384615384615385No Hit
TTATTGGTTCACATAACTGAAAAGTCCAGGGGATATTCTAGCTTCAGGCA11.5384615384615385No Hit
CCTCAACCGCCTTTTCATCAATCGCCCACATCACTCGAGACGTAAATTAT11.5384615384615385No Hit
ATCTTTAAACATTATGGCGAAAGAGCGATCGCATCGTTAAAATCTGCTGG11.5384615384615385No Hit
GGGTTATAATTTTAAATTACCTATGCATCCCCAGCTTTCCTCCTTACCCC11.5384615384615385No Hit
GCTGGGACCCCTCACTTGCCTGCCATGCTCTGCTCGGCTTCAGTCTCCAG11.5384615384615385No Hit
GAATCATCCAGGGAGAAAGGGGGAGAGAATAGATTTTAAAATGCTTAAAA11.5384615384615385No Hit
GCAGCCCACTGCCCCTGATAAAAGCAAAGAGACCAACAAAAATAACACTG11.5384615384615385No Hit
TCTTAGGATATCTGGCCCTAGACAAAGACAAGGAAGGGGGCCGACTCAGG11.5384615384615385No Hit
GTCGGAGCCGTCCCGATAGCGCGCACCGTCACCGAACCGGCCCTGGGCAG11.5384615384615385No Hit
CCGTTTAAAACGCACCCACACCCGAAAAGAAACCAGATTACCCCTCAACC11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)