FastQCFastQC Report
Tue 10 May 2022
EGAF00003641011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2310.9284939105269504No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1590.6390932111419269No Hit
GTAGTAGACAGCCTTGCTCGTGGTTGTTGCGGACGTTGCGCTTCCGGCCG990.39792596165440736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.2733228827525222No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA530.2130310703806423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGG530.2130310703806423No Hit
ATTATAAATAGTGCTGCTTTGCTTGGTGCGGTTGCTCACACCTGTAATCC440.17685598295751437No Hit
GTGTTGGAAGTGGGGGCTAGTGGGAGGTGTTTGGGTCATGGGGACAGATC400.16077816632501304No Hit
CATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGGATA350.14068089553438645No Hit
CCATAGAAGAAGCCCAGTTTCCCTGAGGCTATCATGCTGGAGAGATCACA350.14068089553438645No Hit
GTACATGGGTGAGCCAAGACCCCACCTTTGCACTCCAGCCTGGGCAACAA350.14068089553438645No Hit
CTCCCAGACATTGCAGATGCTGTCATACCTTTCCATGGAGCCCATGAGCT330.13264198721813578No Hit
GTACATGGGGTGTTTGAGGCCCTCATGGTTTGGATCAAGCATGACCTCCA330.13264198721813578No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT310.12460307890188513No Hit
TCATTACACAGTGGCTGCTATAGAACCCCCAGACTTCTGTGAACACAGTT310.12460307890188513No Hit
CCATAGCGCCGGACCCTGCGGCACGGCGGCATCGGCCCCGACCGGTTCAC300.1205836247437598No Hit
GTCTTGTTCTGTCCCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCAA300.1205836247437598No Hit
GTCTACATCTTCTCCCTGAAACTCAATCAGTGGAGGCTGGGGGAGCCCAT300.1205836247437598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.11656417058563448No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT280.11254471642750914Clontech Universal Primer Mix Long (96% over 26bp)
TGTGATGACCGTGCGCGCCGAGATCTCCGAGGCCGACGTGAACCTCGTGG280.11254471642750914No Hit
GTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACAC280.11254471642750914No Hit
GTCTACTACAACGCCCTGTTCGACGTGCCCAACGACCACGGAATCCTGCG270.10852526226938382No Hit
CTTATGGGCAGTGCTTCTGTGGGAGTAGCGGGCCACCAGCATGGGCTCCC260.1045058081112585No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG260.1045058081112585No Hit
GTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACACATACTCAT260.1045058081112585No Hit
CTCTTAGAACCCTCAGTCTCCATAGAAGAAGCCCAGTTTCCCTGAGGCTA260.1045058081112585No Hit
ATCTTGGAGACCGCCAAGAGACAGATGTTCTCTTTGTGTGGCACCACCGT260.1045058081112585No Hit
GAGTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGT250.10048635395313317No Hit
GTACATGGGCAGTTTGAATATTTGTTCCTTCCAAATCTCATGTGGAAATG250.10048635395313317No Hit
GGTGTACGCAGGCCGGTCTGCACCTGGCGCTCGACCACGTCCCCGTCCGC250.10048635395313317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)