FastQCFastQC Report
Tue 10 May 2022
EGAF00003641012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2320.9325133646850758No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1620.6511515736163029No Hit
GTAGTAGACAGCCTTGCTCGTGGTTGTTGCGGACGTTGCGCTTCCGGCCG790.31753687849190076No Hit
GTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGG750.30145906185939947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.26126452027814623No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA520.209011616222517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTATAAATAGTGCTGCTTTGCTTGGTGCGGTTGCTCACACCTGTAATCC430.17283652879938904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT390.15675871216688772Clontech Universal Primer Mix Long (96% over 26bp)
GTCTACTACAACGCCCTGTTCGACGTGCCCAACGACCACGGAATCCTGCG380.1527392580087624No Hit
GTCTACATCTTCTCCCTGAAACTCAATCAGTGGAGGCTGGGGGAGCCCAT380.1527392580087624No Hit
GGTGTGCACCACCCAATTTCTTTAGCTGAGTCCTGGTTGCTTAAGCTGTG340.1366614413762611No Hit
GTCTTGTTCTGTCCCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCAA340.1366614413762611No Hit
CCATAGAAGAAGCCCAGTTTCCCTGAGGCTATCATGCTGGAGAGATCACA330.13264198721813578No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC320.12862253306001045No Hit
TCATTACACAGTGGCTGCTATAGAACCCCCAGACTTCTGTGAACACAGTT300.1205836247437598No Hit
GTGTTGGAAGTGGGGGCTAGTGGGAGGTGTTTGGGTCATGGGGACAGATC300.1205836247437598No Hit
CCATAGCGCCGGACCCTGCGGCACGGCGGCATCGGCCCCGACCGGTTCAC290.11656417058563448No Hit
GTACATGGGTGAGCCAAGACCCCACCTTTGCACTCCAGCCTGGGCAACAA290.11656417058563448No Hit
GTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACAC280.11254471642750914No Hit
CATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGGATA270.10852526226938382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10852526226938382No Hit
GTATGAGATTGGTCATTTTCCTTCTGCCTTTTGAAGACCGTGCTCCAATG270.10852526226938382No Hit
ACTCACTACAGCGTCGATCTCCTGGGCTCAAGCAATCCTCCTGCCTCAGC270.10852526226938382No Hit
GAGTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGT260.1045058081112585No Hit
GTGTTTGGGTCATGGGGACAGATCCCTTATGAAGGGATTTGTGCCCTCCT260.1045058081112585No Hit
ATCTTGGAGACCGCCAAGAGACAGATGTTCTCTTTGTGTGGCACCACCGT260.1045058081112585No Hit
GCCTACCATAGCGCCGGACCCTGCGGCACGGCGGCATCGGCCCCGACCGG250.10048635395313317No Hit
GTACAAGGCCTCTGCCATCACCTTGCACCGCAGCGTCTATGTGCTGGGGG250.10048635395313317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)