FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCCCTGCCTCCGGCCCAGAGCCACAGTGTTCATCCCTCGTGGGGGGC23.8461538461538463No Hit
CCTACTGAATCAGAAACTATGGAAGGTGGGGCTCAGTATTCTATGCTTGG23.8461538461538463No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG23.8461538461538463Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATACAACATTGTGAGTTATACAGTTTTTTCTTGGTGTTTTCCTGGAGTAG23.8461538461538463No Hit
GGCAGGGACCTAGCAGACTGTGTTGTCCCAGGCTCCCCAGGGATTCTGAT23.8461538461538463No Hit
TGTGTGTATTCCCACATAGACATACACACACAAAGAGACACACATAGGGA11.9230769230769231No Hit
GGCCGGGACCTAGCAGACTGTGTTGTCCAAGGCTCCCCAGGGATTCTGAT11.9230769230769231No Hit
GTGCCTGCTGTATCAGAAGCAAAAGTAAATCCTCCCCAATTATTCCTAGA11.9230769230769231No Hit
GAATTTTGCTGTCCCTTGTTTGTCTCCTGATTTGCATCTTCACATTTTGC11.9230769230769231No Hit
TGTGTACATATTCTGACTTCTTTCCCTTCACCGTGGTATTTCCATTTTTC11.9230769230769231No Hit
ATACAACATTGTGAGTTATACAGTTTTTTCGTGGTGTTTTCCTGGAGTAG11.9230769230769231No Hit
AGTGGAAACGGCGTCACCATGATCGCACGGCGGAACCCAGAACCCTTACG11.9230769230769231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11.9230769230769231No Hit
GTAGCAGTCAAAGTCATGGTTCAAGAAGGGTCACTAACATAGCACTTTCC11.9230769230769231No Hit
AGTAGGAATAGACGTAGACACACGAGCAGATTTCACCTCCGCTACCATAA11.9230769230769231No Hit
CTGCAGCATAGAACCTCGCCACCTCCTCATTGGGCTTGCCCTGGGCTGGG11.9230769230769231No Hit
GTAAATAAATTCTGTTCTTTAATGCTTTCAATTTTATCAACAGGATTAGC11.9230769230769231No Hit
AACCAGAAGAGAGAGGAGAGCCTCAGGAGTTAGGACCAGAAGAAGCCAGG11.9230769230769231No Hit
ACGCTGGAGGGGAGGCTGGAAGCGCACCCGTTCCGGGAAACTGGTCCCCT11.9230769230769231No Hit
GTCTCACTCTGCCACCCAGGCTGGAGTGCAATGGCGCGATCTCGGCTTAC11.9230769230769231No Hit
GTGCAGCATAGAACCTCGCCACCTCCTCATTGGGGTTGCCCTGGGCTGGG11.9230769230769231No Hit
AATGAATTATTATTTCTTCATGTTTATGAATTTGATGAAGTGATGTTTCC11.9230769230769231No Hit
GCTGGGTACCATGCAGGCAGTGATTCACCTGCAGTTACCGGTAAATATCG11.9230769230769231No Hit
GCAATTTGATGAAATTGAAGCAGTTGGGAAATCACTTTTGGATAGATTAA11.9230769230769231No Hit
GAATGAGTGAGTGAATGAACCTGGGCCAGAGCATGGGTTCAAACCATGTG11.9230769230769231No Hit
GCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGGCCCACG11.9230769230769231No Hit
GCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAGGCAGGCCAACG11.9230769230769231No Hit
GAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTC11.9230769230769231No Hit
TGTTTAAAGAGGAAACCAGAAAACCCTTAAATTCACCTTTCTCTCTGGTT11.9230769230769231No Hit
TTCCTAAGGCACTCTGTGCAACAGGAGCGGGTGGACGTGGGGGTGGGTGC11.9230769230769231No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA11.9230769230769231No Hit
GTGGAGCATTCAGACTTGTCTTTCAGCAAGGACTGGTCTTTCTATCTCTT11.9230769230769231No Hit
GGCACTTTCTTCCTTTTTAAACCTTTCCAAAGGTCTTTTTGTTAAATACA11.9230769230769231No Hit
CAATTGAAGAAAATATTAAAATCTTTGAAGACGAAGAAGTTGAATTTATC11.9230769230769231No Hit
AGTAGGAATAGACGTAGACACACGAGCATATTTCCCCTCCGCTACCATAA11.9230769230769231No Hit
GACTGTCACTGCCACTCCTTTCTGCCTTTGTGACTAAGAAAGTCACTTCG11.9230769230769231No Hit
GCACATTAGAGAGTAAATGGACTTTTGTGTGATAAATTGGGTACCTAATC11.9230769230769231No Hit
ACAGACACTTTACGCCGGTTTTCCATACCCGTCCCTGAGATGTTTTGGAG11.9230769230769231No Hit
TTTCAGTTCTTCATAGATGGGAAGAACAGCAGGATGACAGACAAATGAAA11.9230769230769231No Hit
CCCCATCTCTGCTAAAAATACAAACATTAGCTGAGAGGAAATGATGTGCA11.9230769230769231No Hit
GGTCCCAGTACTGTAGGAGAGAATTAAATAAAATAAAATAGCTGTAGATA11.9230769230769231No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG11.9230769230769231No Hit
GCTTCCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGGACTTACTTACATATATATATAGGCAGGTTCTGGAATCTTCCCAT11.9230769230769231No Hit
GGATCAGCAGCTCTCCTCTTATAGGTCTTCGTTACTTTATCAACATCAGG11.9230769230769231No Hit
AGCCTGGAGTGAGTGCAGTGGGGTGATACTAGCTCACTCAGCCTCAAATT11.9230769230769231No Hit
CCCATTCATAGAGCAGGTTGGAAACACTCTTTTTGGAGTATCTGGAAGTG11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)