FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCATAAAGATGAGCTAGGGACCTTAAGACCAGAGAAATGACATGAGA35.769230769230769No Hit
GCTCCTGGGAAACTCTCACGATTCATGAAGACTGAGAGCTCAGGGCCCCT23.8461538461538463No Hit
GGTGAATAGTGGCCCCCACTCGCTTCTGGCTTGTAGGGTTTCTGCCGAGA11.9230769230769231No Hit
CTGATGAGTCTCTCATCGCTTGTAAAGCCTGAGACAGCTGCCTGTGTGGG11.9230769230769231No Hit
GGAATGAGCCACTGTGCTCAGCCATGGTATTCTTAAAACAGCAGATTTTT11.9230769230769231No Hit
GTACTGAACGTGCCCCTGTGCAAAGAGGACTGTGAGCTATGGTGGGAAGA11.9230769230769231No Hit
CTCTTTAGTCCTGCATTTTACATTCAACAAAGCAGTTTGAACACAGACGC11.9230769230769231No Hit
ACATTTTACGAACATGTTGAGTCAGAATTGCTTCATACCTACTCTTCTAT11.9230769230769231No Hit
GCTTAGTGTTATTTATTTATTTTTCTGTTGAGAGGTGGGTGCTAAAAATC11.9230769230769231No Hit
CATAAACCATAAAAGCAAAAATAAAAGGTCTTGTATCTTTAAAAACTAAG11.9230769230769231No Hit
CGGCAGTAATGGTACCGGCCGCGCGGCCACCCAGGTTGCTAACGTCAGCA11.9230769230769231No Hit
GGATAACAATCATCCGGGCGAGGTTTGAGGAGGTGCTGGCCTGGGCAAAG11.9230769230769231No Hit
CATAAACCATACAAGCAAAAATAAAAGGTCTTGTATCTTTAAAAACTAAG11.9230769230769231No Hit
GTCCCTAAATATTAAGGAAAGCTGTGATAGTTAATTTTATGTGTCAAATT11.9230769230769231No Hit
CATGCAATGTCACGGAATAAAAAGGACGAGGCCTCCTTGGATTAAGGTCC11.9230769230769231No Hit
TATTATAGTGATCATAGCGTTACAGCTATTGCGATTGACCGCTGCTCTCT11.9230769230769231No Hit
GGAAAGGACTTGCTGTTGTAAGCATTCTTAACCAGCAAAGTACTGCAAGA11.9230769230769231No Hit
GCATTGTATTAGCAAACTCATCACTAGGCTCACTTCCCCTATGTCTAGTG11.9230769230769231No Hit
AGGTTCCGGCCTCGATGTTAATAAGTAACTCCAGTAGGTTTCTGGGTGGC11.9230769230769231No Hit
GCCACCAAGATCGTCATCCCAGTTGGGGCCCCAGTCGATGGTGTCCTTGT11.9230769230769231No Hit
ACCACCACCTCCTGGGTTCAAGTGATCCTCCCATTTCAGCCTCTTGAGTA11.9230769230769231No Hit
GCGTATTGTTATTTATTTATTTTTCTGTTGAGAGGTGGGTGCTAAAAAGA11.9230769230769231No Hit
GAAGACAATCCGTGGAAAAGTCAGGAACAGGTACTGAATAAGAAAGACCG11.9230769230769231No Hit
GTATTCTAATATAGGCCTAAACTTAGATATTATGTACTTACCTCAATTCC11.9230769230769231No Hit
GGGGAATGTTGGCCCCCCCTCTCTGCTGGCTTGTAGGGTTTCTGCCGAGA11.9230769230769231No Hit
GGGTGAAAGTTTACAGCAAAATGAGTAACAAATGAGGAAAACAAGTGAAA11.9230769230769231No Hit
TTTCCTACGTGAATGTTCACTCTCAAAAACCTCCACCAGCATGATATAAA11.9230769230769231No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.9230769230769231Clontech Universal Primer Mix Long (95% over 21bp)
GTAGGGGGGGAGGGCGTGGTGGGCCGATCGCGCGTTGTGGAGATCGAGAC11.9230769230769231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA11.9230769230769231No Hit
TCTGGATGTAATTTCATATATCCAAACATTTCACGGTCCCTCACAGCATC11.9230769230769231No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA11.9230769230769231No Hit
GTACTGAACGTGCCCCTGTGCAAAGAGGACTGTGAGCAATGGTGGGAAGA11.9230769230769231No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG11.9230769230769231No Hit
CTTGACATCTCTCCTAGGGGAAGAATATCACAGGCTAATAGCGTGGTTGG11.9230769230769231No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAATAAAAAAAA11.9230769230769231Clontech Universal Primer Mix Long (95% over 21bp)
GCATTGTATTCGCAAACTCAGCACTCGGCTCACTTCCACTATGTCGAGTG11.9230769230769231No Hit
GGGGTGTGTGTCATTAGTAGCCACAGTCACTGATGGAGAGCTCCTTTTGC11.9230769230769231No Hit
GGGTGCGCAGTGGTATCAACGCAGAGTACCGGGGTAAGCAGTGGTATCAA11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCTACACCGCGTGGAGAAGGTCCACTGGGGCACCCGTTACGCCATCACCA11.9230769230769231No Hit
AGAGGACACATAACAAATATCAAAGTTTATGTCTGATTTATTTGGTGTGT11.9230769230769231No Hit
TTTATAGGTCCGACAGTGCTGGAAGTCTTCAATACCCTTTTGAAACATCT11.9230769230769231No Hit
GGGGAGGGGGAGTGAGTTCGTTTTCTGTTGAGAGGTGGGTGCTCAAAAGC11.9230769230769231No Hit
CCTCTAAGTTGCCAGCCCTCCTAGAGCTACCTGTGGAGCAACCTGCTCAG11.9230769230769231No Hit
TAATTATGCCTTGGGAGTAGCAAGGTGTTAACATGTAAAGAGAAATGTGT11.9230769230769231No Hit
TTTCAATCTGGGTTAGAGTAGAAAAACTGAAAAGAAAAAAAACTGAACGA11.9230769230769231No Hit
ATACTTTTTACTTGAGAAGATGGGAAAAAAAATTCTTTGGATAAGAATTG11.9230769230769231No Hit
GAATGCTGCTGTCTAATTTTTACATGTAAGCCCGTTTCCAACGAAATCCT11.9230769230769231No Hit
GTATGTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)