FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC43.149606299212598No Hit
ACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCA32.3622047244094486No Hit
CTTTCTTTTCTGTTGCCTGTGCTTTGAAGTCTTTGCCATAAAGTCTTCGC21.574803149606299No Hit
GCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGAGCCTGAGAAACGGCTACC21.574803149606299No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21.574803149606299No Hit
ACTGACGGTTGTTGGTTTTGGAGGTCAGAATGAACCGCGTCACCGTTAGT21.574803149606299No Hit
GCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACT21.574803149606299No Hit
CTTTTATAATAGAGTTAAGAATTTCTACCTTCCAGAAAAGCAAAAATTAA21.574803149606299No Hit
GAAGCCTGTGGTCTTTCTACCATCATCGGGTCGGAACTGTCGATTGGTGT21.574803149606299No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT21.574803149606299No Hit
CCTAGAACCTCTGGCGCCACCATCTCTGAGCTTTCTTTCTGAGCTGGTGA21.574803149606299No Hit
TCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGT21.574803149606299No Hit
GCGTCACCATGGTGACGACCACGCCCGCCGGCGACGACCCGGTGGCGTGG21.574803149606299No Hit
GCATGGGAAAGACCTGCCCCCATGATTCAATTACCTCCCACTGGGTCCCT21.574803149606299No Hit
GCACTCCAGCCTGGGAGACAGAGCTAGACTCTGTCTAAAAAAAAAAAAAG21.574803149606299No Hit
CAGGCGACCTTCATGCCGCCGTAGGCCTCCGGGTCGACGCTCAGGTCGAC21.574803149606299No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT21.574803149606299No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG21.574803149606299No Hit
GGTTATGATTATACACGTCAGGACGCGCCGCCACGATCGACTCGACCGCC21.574803149606299No Hit
GTCTCAGGCAACTCAAGTTTAGACCCTTACTCTTTTTGTTTGTTTTTTGA21.574803149606299No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC21.574803149606299No Hit
AATATTTGATCATAACTGTTCCTTATGCCTGATTATAACAGTTAACCGCC21.574803149606299No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC10.7874015748031495No Hit
TTACTGCTGGGGTAAGCAGAAGATTTCACTATTAGACTTGAAAAAGGGAA10.7874015748031495No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT10.7874015748031495No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA10.7874015748031495No Hit
GCTATTCCCACTCCTAGCTCCTTCATGTATTCATCAAAGCCTTTGCTGTC10.7874015748031495No Hit
GCTTTGCTCAGTGCTTTGCCCCAGGATTGCCTCAAATCTGAGTGGACTTC10.7874015748031495No Hit
GTTTGGGAACGCGGATGCGCAAATCTGTCGAGGGAGTCTCCCTTACAGCA10.7874015748031495No Hit
GCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATC10.7874015748031495No Hit
TTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT10.7874015748031495No Hit
CCTCAACTCCTGGCTCAATACTGATGGAAGTAATCTGCTTAACAATCTCA10.7874015748031495No Hit
CCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGCGGCTCCTGCAG10.7874015748031495No Hit
GGATGGGGTCAGTGGATGGAAAATTACTAAGAGAAAACATCAAGAATAGG10.7874015748031495No Hit
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAT10.7874015748031495No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA10.7874015748031495No Hit
GATGCTAAAAGTGGCAGATCATTCAGGCCAAGTAAAGACCAAGTGCTTTG10.7874015748031495No Hit
CAGCTACACCTACCGGCCCTGGGACGCCGGAGGACAGGGCATTGTCCTCA10.7874015748031495No Hit
GACCATGGCCATTATGAAGATGTATGAGCTTTTCCCTTGTACCCAAGGGG10.7874015748031495No Hit
GCACAGAGGGAAGGGGTGCCCAGTGACCACTGGTCCTGGAGGTTCTGTGT10.7874015748031495No Hit
TCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC10.7874015748031495No Hit
ACCACCAGGAGTGGAGCCTGCGGCTTAAATTGACCCAACACGGGAAACCT10.7874015748031495No Hit
CTTTTATACTAGAGTTAAGAATTTATACCATCCAGAAAAGCAAGAATAAA10.7874015748031495No Hit
CTTCACATTAGATTATTATGCTTATTGTACAGATGAGAAAAATGAGGTAC10.7874015748031495No Hit
GGCCGAGCAGGCGCCCGACATCCTGTGCCTGCAGGAGATCAAGTGCGAGG10.7874015748031495No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA10.7874015748031495No Hit
AAATAGGACTGGAAACAAGGGGCTATAAATTAAACGGTAGAGATTCCAGA10.7874015748031495No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT10.7874015748031495No Hit
ATAATATTCCACCCCAAATTATAACTCAAAAGTGCTAAAAACATATTCTT10.7874015748031495No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTG10.7874015748031495No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC10.7874015748031495No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT10.7874015748031495No Hit
CCATGGTAGTCACGGCGACTACCATCGACAGTTGAAAGGGCAGACGTTCG10.7874015748031495No Hit
CCCGGGAAGCCTTTTCCCGGAAGGATACAACCGTCTTGGTGCGGCTGGAG10.7874015748031495No Hit
CACCCTACCATGGAGACCACGGGTGACGCGGAATCAGGGATCGATACCGG10.7874015748031495No Hit
TCCCGACAGCGGCGCCACGACCATGTTGGCGAGCGGCCATGGCGCGATCG10.7874015748031495No Hit
AGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCA10.7874015748031495No Hit
TCTCCAGCTCCATCCATGTACCCATGTTGCTACAAAGCTCATGTCTCATT10.7874015748031495No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG10.7874015748031495No Hit
ACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCT10.7874015748031495No Hit
ACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAGTAT10.7874015748031495No Hit
GATGCCAGAGGCTCTTGAAGTCACAAAGGGGAGGCGTGAAGAAATCCTGC10.7874015748031495No Hit
ACCAAATCCTGTTGCCCCGAGCGTCGCGGCGCGAGACGAGTAAGTAGCCC10.7874015748031495No Hit
GGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAA10.7874015748031495No Hit
TCTGACAGAGATGTCATCTCATCTCCAGACTGGGAGGTATGATAGCGCAG10.7874015748031495No Hit
GAGCTGGGTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAT10.7874015748031495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTGAGACAGGGTTTCGCTCTTGTTG10.7874015748031495No Hit
GGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGC10.7874015748031495No Hit
TCTATAAACCCGATGAACGTGTCTTTTCCACTTAAGATGTGAACACACTG10.7874015748031495No Hit
CGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGC10.7874015748031495No Hit
GAGCGAGGTGACGATCTTGCGCTTGACGCGGTACGACGGCGTGAGCCGGG10.7874015748031495No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7874015748031495Clontech Universal Primer Mix Long (95% over 22bp)
CCCCCGGCCGTCCCTCTTAATCATGTCCTCAGTTCCGAAAACAAACAAAA10.7874015748031495No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT10.7874015748031495No Hit
CAGTACGACGATGATGGACCCGTCGGGCCGGACGAGTTGAGTGCGGGTGC10.7874015748031495No Hit
GAATAAGTGTCATGAGATCTGATGGTTTTATAAGGGGGAGTTTCCCTGCA10.7874015748031495No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGG10.7874015748031495No Hit
GCTCCACGCAGTACATGAAGGAGTACCAGGACGCCTTCCTCTTCAACGAA10.7874015748031495No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG10.7874015748031495No Hit
ACCATGCCCAGTCTACTTTTTAAAATCTGATTCCCATTCCCTCTCTGTTC10.7874015748031495No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG10.7874015748031495No Hit
ACCATGATCGCCACGCTGGTGCAGCTCATGGGCGAGGACAAGGCCTTCGA10.7874015748031495No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG10.7874015748031495No Hit
CGATAGGATCTCCGGTGCCGGCATTCATGCTAATATCCTCCCCTGTCTCT10.7874015748031495No Hit
GAGTCCAGATGAATGACCTCGGATCCATCAAGATCACAGACGTAGCCGTC10.7874015748031495No Hit
CCATATTGGCCAGGCTGGTCTAGAACTCCTGACCTCGGAATCCACCTGCC10.7874015748031495No Hit
GCCCGGTCATAACGGCGCTCACGCCCATGACGATCCCCGATGTGTTTCCG10.7874015748031495No Hit
CCATCGTCAGGTCATTATCCCTGGCCATGGCGACGGCAGTTGCGTCGGCG10.7874015748031495No Hit
GGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTT10.7874015748031495No Hit
CCCTCACTCTCTCTTGTTCCCCCCTTGCTGTGTTATGTGCCTCCTTCTGC10.7874015748031495No Hit
CTGGTTGAGAGACTGCACCCACGAAAGATCTGTGCCGTCAGGCTGAATCC10.7874015748031495No Hit
ATGTAAGCATGCCAAGGCAGATATTTAGGCTAAATTGTAGGAGCTAAGAA10.7874015748031495No Hit
CAAGAGAGGCAGTGGAGCAAACTGCCAGTTGAACAAGTTAGACTGCCTGC10.7874015748031495No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGATGCGTGCATTT10.7874015748031495No Hit
GTCTTTGGGAGATCACCTCGTTGCGACTGGAACAGTTGGGGCATCCCGTA10.7874015748031495No Hit
TGGTTTTATTTTCTACTTCCTAACTTTCATCCAAGATTTCCAGAGTGTCA10.7874015748031495No Hit
CCGTCGCTGCCTTCCTCGTCGTCGTACCCCCCGGCCCGGACGCGCACGGC10.7874015748031495No Hit
GAATAGAAGCATCGAATGAAATCAAATGGAATTATCATCGAACGGACCCA10.7874015748031495No Hit
CCCCTCCTCAGTTCACAGTGGAGACTAAGGAGATTCAGGGCAGGATCCCT10.7874015748031495No Hit
CATAAGATTTCCAAATATGCCCTACACCTAGGCCATTTTTTTTTCTTTCC10.7874015748031495No Hit
GGGATGCGTGCATTTATCAGATCAAAAACAACCCGGTCAGCCCCTCTCCG10.7874015748031495No Hit
CTATCCCTCAGCCCCGCGCGCGCATTGAGGCGGATCAGCGGCCAGAACAG10.7874015748031495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)