FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATTTGGGGCTTTGGATGACTTATCTGCAAGTAAAATTCAGAGTGG36.8181818181818175No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36.8181818181818175Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTGGAAGCCGCGCACCTGGCGGGACGCGACATCACGATCCTGTCGGC36.8181818181818175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24.545454545454546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGTATCAACCCAGAGTACATGGGAAGCAGTG24.545454545454546No Hit
CTCTAATCTTGTGGCTTTTGTTAATCCTACAAAGGCCTGTCTCTTATACA24.545454545454546No Hit
CAAGGATGCCGGAATGGTGATCCTCGGCAAGACGAATCTTGATGAATTCG24.545454545454546No Hit
GTTGTTATGGGAAGATATTTCCTTTTCCAACATAGGCCTGAAAGCGCTCC24.545454545454546No Hit
GATCGGTCATGGGTGACCTGAGCATGGTCAGATACGAGGGCTACAGCGCC24.545454545454546No Hit
ATAAAGGCCAGAGTGGTATTAAACTTACGGGGCCAAAAGATCTAGAATCC12.272727272727273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT12.272727272727273No Hit
AGCTTACTCCAGAACTTAACTTGCTGGGCAATACCTAATTCCGCCGCAAG12.272727272727273No Hit
AGCAGGGGATGAAACACGAATAGAAAGATATGAATATTTTGTTGAAGAAT12.272727272727273No Hit
CGGTGGCTCCATCCGGGTCGAACCGGCGAAAGGCGGGATTGACCGCGACC12.272727272727273No Hit
CTGCGTTGAAACCACTGCTTCCCATGTACTCTGCGTTGATACACCATGTA12.272727272727273No Hit
GGCCTGCGCCGTCGCAAGCGGCACTGTGGAGCTATCCTGGAGGCTGGCTT12.272727272727273No Hit
GCTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTG12.272727272727273Clontech SMART CDS Primer II A (96% over 25bp)
GAGAAACATGTTAAGTATGTTCCTTTTTAATGATTTTTTAAAAAAATATA12.272727272727273No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GATGGCAGATTTTAGCGACAATGCAGTTTGCCAATGTGGTGCAGATAGCT12.272727272727273No Hit
CACCCTCACCACCAAGGGCCTCATAAATCTTCTGCGCCGAATGAGGCATG12.272727272727273No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTA12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTCGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGG12.272727272727273No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCTTGCCGCCCAGCTTGGGATGCGCGGCTGGCATTTTTTCCGCTTCGTC12.272727272727273No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA12.272727272727273No Hit
CTCCTAGCCTCTGCAAGGTTAGATCACGAGGTAGCTTACTCCAGAACTTA12.272727272727273No Hit
GAACGGCCTGGCTGAGCACGCCCGCCTTTGAAAGACTGAATGTTATGCTG12.272727272727273No Hit
GTGCTGGTAGAGCCGCACGACGACGCCTACACGATCTTCGCGCAATCCAT12.272727272727273No Hit
CCCAAGTACTCTGCGTTGATACCACTGATTCCCATGTACTCTGCGTTGAT12.272727272727273Clontech SMART CDS Primer II A (96% over 26bp)
TGTTTGCCCACTGTATGGAGTGGGTTTAGAGCTGTCATCGGCTCTTGAAA12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)