FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCAGCTGAGCAGCGACCGGGTTCTGCCCAACGACAGAGCCAACAGCT36.8181818181818175No Hit
ATTTAAATCTCCTCTTAAAAAGCAGAAGGAGAGAAGGAAACATAAGAAGT36.8181818181818175No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG24.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGGGTT24.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
GCGCAGCATCAGGCGGTCCACGGCTTCGCTGGCAAAGGGCTGGGGCGCTG24.545454545454546No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24.545454545454546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAGAAACATCTTTGTGATGTTTGTATTCAGGACACAGAGTTGAAC24.545454545454546No Hit
GCCTTTGTAGGATTAACAAAAGCCACAAGATTAGAGCTGTCTCTTATACA24.545454545454546No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
TTACTGCTCAACGAAGCATCGTGGCTTTAGCAGCTACCAAAGAGACAGCT12.272727272727273No Hit
CAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCG12.272727272727273No Hit
GGCCACACGCACCGGGCCTACGCCTGCGAGTACCAGCGCCCCAAGTCCAC12.272727272727273No Hit
GTCTCCCCGTGTCGGAATCCGGCAAGCAACTCCAACTCGTCAGCTTCGAA12.272727272727273No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT12.272727272727273No Hit
GGTCAAATCGGTATTGATGAAAAGCTATTGGCGGCTAATGCTGACCATGA12.272727272727273No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCA12.272727272727273No Hit
CCCCTTGTTTGGGAAAAACCTTTTCGTTTTGTTTCCATAGGCAGGCTCTT12.272727272727273No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
TACCACATTTTTGGATAAATTTAACTTATGAATTAATTCATTCCTTTCAC12.272727272727273No Hit
GAAGCCGCTCTCGCCGTTCCCAAGCAGGCGGTTCAGCGTGACGCGAGCGG12.272727272727273No Hit
AGCCTAAGTATCACAGGACAAGCCCATTTAACAGACAGCCAAACTGACAA12.272727272727273No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGGCTTGATCCCGACGGTGCCGGTCACCGCACCAGGTTGGCGGATCG12.272727272727273No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
CTGCATGGGACTGTGGTCCATGTCCATGCCGGCCATGGAGCCGTGACCCA12.272727272727273No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG12.272727272727273No Hit
GAGTGACCATAAGCAGATATTTGTGAAGAGGTTAATTGCCTTACCAGGTG12.272727272727273No Hit
GACCGTGAGTTGCTGGATGCCGCTGCTGCTGGGTTTGACACCGTGGGCGG12.272727272727273No Hit
CAATTATAGATGTTGTGGATTGCAGGAATAATATTTGTATTATAAACATT12.272727272727273No Hit
GGTCGGCTTGATCCCGACGGTGCCGGTCACCGCTCCAGGTTGGCGGATCG12.272727272727273No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGC12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)