FastQCFastQC Report
Tue 10 May 2022
EGAF00003641143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516205
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA11120.2154182931199814No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11110.2152245716333627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10890.21096269892775157Clontech Universal Primer Mix Long (96% over 26bp)
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA10750.20825059811508995No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA10670.20670082622214042No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT9300.18016098255538013No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA9300.18016098255538013No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7270.1408355207717864No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC7090.13734853401265001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.13618620509293788No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT6970.13502387617322575No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC6780.13134316792747067No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6720.13018083900775854No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT6640.12863106711480904No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6390.12378802994934184No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6320.12243197954301102No Hit
TAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCT6090.11797638535078118No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6050.11720149940430644No Hit
GTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCATTGC5610.10867775399308414No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT5440.10538448872056644No Hit
CATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTAA5310.1028661093945235No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5180.10034773006848054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)