FastQCFastQC Report
Fri 10 May 2024
EGAF00003641161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111
Total Bases11.1 kbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGTAGGTCAAATAATATTTTCTGAAGATAACAATTATGGACTTTAAA32.7027027027027026No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC32.7027027027027026No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG21.8018018018018018No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG21.8018018018018018No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA21.8018018018018018No Hit
GAAGCAGAGAGTGGCTGGCGGGGTCCAGAGGCCTGAGCGAGGGAAATAGG21.8018018018018018No Hit
TGAGTGATCTTATAGATTCTGGAGAAAATATTTAAAGGAGAGGTTTTTCT21.8018018018018018No Hit
GCGGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.8018018018018018Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TATCAACGCAGAGTACATGGGCGACAGCGACGCCGCGAGTCCGAGAGAGG21.8018018018018018No Hit
GGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTA21.8018018018018018No Hit
GATTACAGGCGTGAGCCACCGTACCCAGCCAGGTATCCTGATTTTCTATC21.8018018018018018No Hit
TTAACAAGAGATGCTGGATTTGTCCTCCCTGTGGCACAAACTGGGGCAAC21.8018018018018018No Hit
TGCTCCTTGTTCTGCAGCTTGGTGCGGATGGACATGATAACTTGGCCAAT21.8018018018018018No Hit
GAACGCTTCGAAAGGGCCAAAGAAGCCCTGGCCGACGCCGTGCTGATCGC21.8018018018018018No Hit
GCCAGGAACACTCCTGTTCCAGCCACTGCCCCAGTCCAGAAGGAATGTTC21.8018018018018018No Hit
TTCTAAATGCACCATATAGTTTTGTATCAGTGCTTTTGCACATGTTTTTT21.8018018018018018No Hit
TCACCCACCGGCGCACTCACCAACTTCCCACCACAAGTCGCCAAACCACG21.8018018018018018No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.9009009009009009No Hit
GTGGCACTGCAGGAAGCTCAGGACACCATGCAAGGCACCAGCCGCTCCCT10.9009009009009009No Hit
AACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCTGCCGTG10.9009009009009009No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCCTCTGC10.9009009009009009Clontech Universal Primer Mix Long (96% over 26bp)
TCCTAGGCGGAGTCATCAGCGCCATCAGCGAGGCGGCTGCGCAGTACAAC10.9009009009009009No Hit
CCCAGTACGTCCTGATCTGCTGCCGGGCCACCACTGGGCTGCACCATCTC10.9009009009009009No Hit
CTTAAAAATATTGCTAAAAAGCAAATGCCTACACACTGGTCTTTGCAGCA10.9009009009009009No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.9009009009009009Clontech Universal Primer Mix Long (100% over 23bp)
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC10.9009009009009009No Hit
TTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGT10.9009009009009009No Hit
ATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC10.9009009009009009No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA10.9009009009009009No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.9009009009009009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTATAAGAGAATTTCCATACTCAAGTACAGCCCAGATTCCATAATGCT10.9009009009009009No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAA10.9009009009009009No Hit
ATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG10.9009009009009009No Hit
GAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAAT10.9009009009009009No Hit
CTAGGATTGCAACCCCTGCCTTTTTTTGTTTTCCATTTGCTTGGTAGATC10.9009009009009009No Hit
GGTTTTAGATGTCTAGACCAACTCCCTTTTTTACAGATGGGAAAACTAAG10.9009009009009009No Hit
GATCTATCTGATATAGGTGCCAAAGAGAGAGCTGATGCCAAGAGATAGGG10.9009009009009009No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACA10.9009009009009009No Hit
GTATTGAATGCTTATCATGGCTTATTGCAGCCTCAACCTCTCAGGCTCAA10.9009009009009009No Hit
CCTGTCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTTTACCTCAC10.9009009009009009No Hit
GTGTGTGGACCGCTCGGGGACGGGTGCGTCACCTGTGAGTTGCTTTGTCT10.9009009009009009No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAT10.9009009009009009Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCACTACGGAAACTTGAGGCACCACACACATGACTTTGCTGACTGGGGGC10.9009009009009009No Hit
CCTCATGGAGAGCCTGACCAGGCCTGCCACAGAGACCTCAGAGTGCCAGA10.9009009009009009No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA10.9009009009009009No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.9009009009009009Clontech Universal Primer Mix Long (96% over 26bp)
ATACTAAACATGTACCCACTTTTTTGTCATTATTCCCTAAACCAGCGGTC10.9009009009009009No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.9009009009009009Clontech Universal Primer Mix Long (95% over 22bp)
AGCGCGCTGTGATCCGTGGCCGACGGGTCGTCGCTCATGAGTTCATCGCA10.9009009009009009No Hit
GAGCAAATGTTTCTGTTTACTGCACTTTTTGTAACTGAAGGTGATAAATT10.9009009009009009No Hit
GGTCAGTGATTATGGGGAAGAGTCAGGGCCCATCAGGGGAATGAAGTGGC10.9009009009009009No Hit
GCCCAATCCTTGCTCCGGGAGTTTCGCGGAGATTCTCATGTCTCACTTCT10.9009009009009009No Hit
CGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGT10.9009009009009009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTATGGTGTAAACAGC10.9009009009009009No Hit
CTCGTGTAGCATGTTGTGGCACCGTGATCCAGTATATGAGCTACCGAGTC10.9009009009009009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.9009009009009009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTTATCCTTCGA10.9009009009009009No Hit
GTACATCAGATTGCTACCTAACAGAGCGAGGAAATCAGTTCTTATTTTCC10.9009009009009009No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.9009009009009009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTCATGCGATTCTCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGC10.9009009009009009No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.9009009009009009Clontech Universal Primer Mix Long (96% over 26bp)
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT10.9009009009009009No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG10.9009009009009009No Hit
GTGCTATGGGGGGCCAGTGGCTTCTGGGAAAGGAAGGGGGAAATGTGTGT10.9009009009009009No Hit
TGTGTATTCTTGTGCATATGTGTATGAATAACTTACGCTGTTCTTTGTCC10.9009009009009009No Hit
TGCCAAGCTAATTGTTCATTACTTGAATTTCTGTGGGCCCGGGGGCAAGC10.9009009009009009No Hit
GGCTGGAGTGCAATGGCATGGTCTCAGCTTGCTGCAACCTCCACCTCCCG10.9009009009009009No Hit
CACCAATCACTCCAGATGGTTTTGGGGGAACCATTCTACTCTTCTGGTGG10.9009009009009009No Hit
GTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAAT10.9009009009009009No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.9009009009009009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGA10.9009009009009009No Hit
GCTTCCCATGTACTCTGGGTTGATACCACTGCTTCCCATGTACTCTGCGT10.9009009009009009Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTCAGTGACTGGACAGTGTGAATGTCGGCCAGGAGTTACAGGACAGCGG10.9009009009009009No Hit
GAACCACTCAGGCTTATTTTTCAGGTTGATATTGATGACTTCATGCCTGA10.9009009009009009No Hit
CGGTATCAACGCAGGGAGGAAAGGAGCCGGAGAAGCCGGAGCAGAAGTCC10.9009009009009009No Hit
GTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG10.9009009009009009No Hit
GCGCCAGGCCTTGCAGCAAGGCGCAGAAGAAGAACGGCTGTCTCTTATAC10.9009009009009009No Hit
GTGAAATGCAGTGTTTTACCAGGATTTACCATAGAAAACAAGGTCCATTG10.9009009009009009No Hit
ACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAAC10.9009009009009009No Hit
GTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTT10.9009009009009009No Hit
CCTTGAAGCCGATGGCCACGAGGGTTTCGAACATGCGCATCTTGCGCGCG10.9009009009009009No Hit
CCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGACTGTCTCTTA10.9009009009009009No Hit
CTGGCACACAGAGCGACAAAGTCACCCTCAAGGGCGCCAAGGACTGTGTG10.9009009009009009No Hit
GATTAAAGTCCTCGCCATCTTCCAGCAGGCGGCGGTAGGTGGCGATCTCA10.9009009009009009No Hit
ACAACAGCGTTACCAGGGTGCGCGACAACAGCACCCAGAACAGGTACAGC10.9009009009009009No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTC10.9009009009009009Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.9009009009009009Clontech Universal Primer Mix Long (96% over 26bp)
TCCCACAGAAAGCTCTGGCCCAAGGGCTTTTGCGTCTGCGCGTGGCGCCA10.9009009009009009No Hit
CAATGGCTGGGCACGGTGGCAGTTTCACCAAGTTGACCAGGCTGGTCTTC10.9009009009009009No Hit
TGTGGGCCCTGTGCTCCCGTCCAGTCCGATGATGAAGGCGAAGGTGAGGC10.9009009009009009No Hit
GAAAAGGAAGCCAATACTTGAGGACCAAGTACAACCTCTACTTGAATTAT10.9009009009009009No Hit
CGTTTATGGTCGGTACTACGACGGTATCTGATCGTGTTGGGACCGGCGGG10.9009009009009009No Hit

[WARN]Adapter Content

Adapter graph