FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACTATCTGGTCCTTTGCAGAAAAAAAAAAAAAGTTTGCCAATCTCT34.3478260869565215No Hit
CATTGTGATAAGCTATATTGAGGAATCATTGAGTTGGTTCGGTGGTTCTG34.3478260869565215No Hit
GTACGCCGACTCGGGCAATTTCGAGCGCTGCATCCGCTTGTGGAAGTACG22.898550724637681No Hit
AAGTTATTTCAGTCATCTTTGTCTTTGGCTCCATGTTTCAGGATGCGTGT22.898550724637681No Hit
GCTGGTGACCGGCGGGCCGCTGCGGCTGGAAAAGAGGCCGGCGCGGGACT22.898550724637681No Hit
GTCTGTTGGTCTTAAAGTTATTGGAAACCTGTGTTATCTTAAACATACTG22.898550724637681No Hit
CCTTGTAGTTGGTTATAGTCATGTGATGAACTTACTACAAGGAAAGCTGT22.898550724637681No Hit
CTCTTTGTACAAGGAAGGAGTCTCCAACCCATTTCCTTTATGACTGAAGT22.898550724637681No Hit
GCCCCACAGCACTTAAAATCTGCCTGAATCCTGTCCAGGATCGAAGCAGT22.898550724637681No Hit
GTAAAATGTTGAAAGCACGGTTAAAATAAAAACAAACCACAGTTACATTA11.4492753623188406No Hit
TCCGAATGGCCGAGGATGGCTACGACGTCCCAGAACAGTTCGACAGCGCC11.4492753623188406No Hit
GTACATGCTCCATGGAGAAATCAGGAATTCTTTCACAAAGAACAGATCCA11.4492753623188406No Hit
CTATTGAAAGTCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCA11.4492753623188406No Hit
CCACATTCGGTGCTGAAATCCGAGGCCCTTTCAGGTTCACATCCACACCT11.4492753623188406No Hit
GTCTGGAGCTGGTCAGCCTGGTGCACGGCTCTTTTTCCCTTTCTTCTCAA11.4492753623188406No Hit
CATTTCCAAGATCTTTGCTGCAAGGAGCATTGTACTCAGCAATTGCAAAC11.4492753623188406No Hit
GTGTACGGATGAGTGACCTGCACTAAGAAGTGAGTTGCCACAGTGAAAAT11.4492753623188406No Hit
GCCTGATGGTATTACATTGTGTCGATAGATTCAGAGAGCATAGGAAGAAG11.4492753623188406No Hit
GTCTTGCCGTGGCCAGGTGGGGTGGCTCATGCCTGTAATCCCAGCGCTTT11.4492753623188406No Hit
CTGCCATGCACTTCTGGAGTGACGCAGAATTGGTCACTCCAGAAGTGCAT11.4492753623188406No Hit
CTCCCCGTCCCTGAAGTAGGCCACCGTTTCGACATGGGGCAGGCCATGCC11.4492753623188406No Hit
GAGCAGGCCTAAGAAGAAAATGCTCTGTGCTAGCTCTGTGTGTGTGTGTG11.4492753623188406No Hit
CTTCATAGCAAAAGAGAGAAATAATGTTTTCAAATAATTACCTTCATTAC11.4492753623188406No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.4492753623188406Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTTCCATTCGATGATGATTCCATTCGTTTCCATCCAATGATGATTCCATT11.4492753623188406No Hit
GCAGAGAAGGCTGGTGGCTCCATGTAACCACCTTGGTAATGGGCTAAATA11.4492753623188406No Hit
CAACTGGAGGATGCGCTGAAAGAGGTGAACGAAGCAACGCACCGCTATCT11.4492753623188406No Hit
GGCCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAAC11.4492753623188406No Hit
TTTTTAAGATGGGTTGTTGTTAACCTTGGCCTTGCTCCTGGTGTGGACAG11.4492753623188406No Hit
GGAAAATGGCTGCTGAGACTTAGAAGTCATGACGGAAACAACTGGTGACA11.4492753623188406No Hit
ATGTTTGGTTTACTGTTAGCGTAGCCACGTTTTGGGGACGATGTGGTGCC11.4492753623188406No Hit
GACGAGGGCGCGGCCGACGGCTTCATCCTCGGCTTCCCGGTGCTGGGGCA11.4492753623188406No Hit
ATATGGTTTTGTATCAATTCCAGTTTCTGCATTTTGATTTTTAGTCATTT11.4492753623188406No Hit
CCATCCAGGGCGGGGAAGGGAGCGGACCCTCCCGGCTCCAGGTGCCTGGG11.4492753623188406No Hit
CCCAAAGACAGCAGAAGAAAAGAAATAACAAATATCAGACAACTAAATAA11.4492753623188406No Hit
GGTATGTGCTCTTAAGAAACGCATTTAACAGAGGAAGAAGCTGAAACTAC11.4492753623188406No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG11.4492753623188406No Hit
AACTGTTAGTCCAGGGGCACTCTAAATTAAATGTTCAGCAAGACTTTTCT11.4492753623188406No Hit
TTCACATACATGCAAGTCATTTCATGGAAAAGCTGTTTTCTTACCTGCGT11.4492753623188406No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG11.4492753623188406No Hit
GGCTGGGAGTAGGAATGGAGGTTGATTGTAAATGGGCACAAAGTTTGATT11.4492753623188406No Hit
AACCCACACCACGATGCGCAACCCGGAGCCAGAAAGCACCGGAACGCCGG11.4492753623188406No Hit
TTGTTATATAGTGGCCTTTCAGACACTAGGTTTTGGGTCCTGTTCCTGGT11.4492753623188406No Hit
GTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAAT11.4492753623188406No Hit
CTATTAAGAGGCCCCTCAAAATAGAAATTTAATTAGGATGCCTGAGAAAA11.4492753623188406No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.4492753623188406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTTCTGTAGTATCTGCAAGCTGACGTTTCAAGCGCTTTCAGGCCTAT11.4492753623188406No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC11.4492753623188406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTT11.4492753623188406No Hit
AAGGAATACAATTGCCTTTGATCCCCTCCTCAAAATTTCATTAACCAGAG11.4492753623188406No Hit
GTTTGCTATCATTATTATTTTGGAGATGAGGAAACTGGGGCATCAAAGTT11.4492753623188406No Hit
TCCTTCGCCCGAACAGATTTTGCAGGTGCGGCAGCAAGCCGCCGAAGCCG11.4492753623188406No Hit
GAACTAAGGACACACTAAGCCCAAGCGTTGCGGCGAAGTTCTTTTTATTC11.4492753623188406No Hit
CCTTCCAGAGAGCCCAGGCTGGCCTTTGAACTTTTCAGGTCACCTTTGGA11.4492753623188406No Hit
TTCCTAGATCTTGATATGAATCTATAAAGAAACCTAAACAATATTGCAGA11.4492753623188406No Hit
TCAATATGGGAACCGGCAATGCGTTCTCCTTATTACCTGCACAAAATGCG11.4492753623188406No Hit
TGCCTGCCCACAAGTTTATCCTCTGCACTAGTATTTGAAATTCCCCGGAA11.4492753623188406No Hit
CCAGAAGAATATGAAAAAGCAGAGCGACTCGGTTAAGGGAAAGCGCCGAG11.4492753623188406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)