FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAATAAATTAGCACAAATTATAGACTACAAGTGATCACTCAATCT34.3478260869565215No Hit
GGGCAGTCCTGTCTTTGCTTACTGAATGATTGGGAAGGAAAATAGAGAAG34.3478260869565215No Hit
GGCCCTCTGGAGGAAGGTGCTGATTGGAATCATAGACTGCAATGGATGAG22.898550724637681No Hit
CTTTAATATAATAGTGAAATGATGATCCTGTTTCACTAGTAGGATAAGGT22.898550724637681No Hit
GCTCTCTGTTGCCAGTGGTCATCATCGCAGTGGGTGTCTTCCTCTTCCTG22.898550724637681No Hit
CCTTTGGTGAGAACCCCCATGAGGTCTGCAAAGCCGATCTGGGTGCCCAG22.898550724637681No Hit
GGTCTGCAGAGCCACAATCTTGGCTCACTGCAACCTCCACCTCCCGGGTT22.898550724637681No Hit
TCCCGCGCCGGCCTCTTTTCCAGCCGCAGCGGCCCGCCGGTCACCAGCCT22.898550724637681No Hit
GTATCTAGATGCCATGATGAATGAAGCTCCAGGCCCCATCAATTTCACCA22.898550724637681No Hit
AATCCATTAGGCGTGGGGAGGGAGGAAAGGTATTTATGGCAAGCGTTTTT11.4492753623188406No Hit
GCCAGAAGAATTCTTCAGATTCTGATTTTCTCTATAAAAAATTCTGAAAC11.4492753623188406No Hit
GGCTATACCGGCCGGACCGCCGCCCGATCGCTGCCCGCTTCGTGCACGAG11.4492753623188406No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT11.4492753623188406No Hit
GCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGG11.4492753623188406No Hit
CAGGAACCATTGATAACGGTATCCTGGCGCTTATTCGCCATACCCGTGGG11.4492753623188406No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT11.4492753623188406No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.4492753623188406Clontech SMART CDS Primer II A (96% over 26bp)
GAGCTGGTTGGCAGAGAAATGGGGGTGGATGTTCACTTCCCTAAAGCAGA11.4492753623188406No Hit
GCTGTGTTTAACTTCCTCTTTCATAGTTACTTACATTCTCATACTATGCC11.4492753623188406No Hit
GTACCGGGATGAATTGGCGGATTTCGCAGCCGCCTGCGCCGTAGTCAAAT11.4492753623188406No Hit
GCCCTGAGGGGATTCGAAGGGCTATTAGGAAGTTCAAATACTGTCTTGGG11.4492753623188406No Hit
TTTGTAAACTACAAAGTAAAATGAACATTTTGTGGAGTTTGTATTTTGCA11.4492753623188406No Hit
CACACAGCTAGCACACACACACTAGGCGCACACACAGCACACACACACAG11.4492753623188406No Hit
TACACAGTCTGACTCCCTGGGTTTTAGCAAGCCATCAACTTAGTTGAAGC11.4492753623188406No Hit
AATGGAATCAACATCAAACGGAATCAAGCGGAATTATCGAATGGAATCGA11.4492753623188406No Hit
CCTCAGTGTCGGGGGCTCGCAGCGCCGCCGCGGCGAACCCAGCTAGGGGG11.4492753623188406No Hit
TTACCTTTGTCTTTTCTTCTCCTATATACCTGCTAATGCTCAGTTCCAAA11.4492753623188406No Hit
TGTTCATTTGTTTTGTCTTTTTGCTACATAGAACATAACAATACATTAGG11.4492753623188406No Hit
CTTCGTATCAATAGTTGCTGTCATCGATAAACCAATACGCAAGGGATGTT11.4492753623188406No Hit
CTTCAGGATTTCTATTTATTTTTCTGATTGAGTCTAGGAGGATTGTATGT11.4492753623188406No Hit
GCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTTTCTGCTTCTGCTGC11.4492753623188406No Hit
ACTCGAAGGTCAGTCCTTCGATCAGCGGCTTCGCGCGCGGATCGAGCGGC11.4492753623188406No Hit
AGATAAGCCTCAGCACCTCCTCTGAGTTCCCTGCTACTTTGTACTTTCCA11.4492753623188406No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11.4492753623188406No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.4492753623188406Clontech Universal Primer Mix Long (96% over 26bp)
AGCTTAACGTTGGGGCACCAGATGTGACACTGAGGGGACCAAGCCTGCAG11.4492753623188406No Hit
CCTTGTGGTCTCTGTTGTAACTACTCAACTCTGCTGTTGTAGCATGGAAG11.4492753623188406No Hit
GCACCACATCGTCCCCAAAACGTGGCTACGCTAACAGTAAACCAAACATC11.4492753623188406No Hit
TATTTAAGGTGCATAAGCATGGGGGAAATTGCAAGACAATTATTACCCAA11.4492753623188406No Hit
ATATTAAAGAAATTGTTGCCACTATTAATATTACACCCCAAGGCTAGAAG11.4492753623188406No Hit
GTCAACGATGACAACAGCACCTGAGCGAGGTTGTCGGCGCTGTCGAACTG11.4492753623188406No Hit
GACAGAGTCTCGCTTTGTCACCCAGGCTGGAGTGCAGTGACACAATCTCG11.4492753623188406No Hit
TTCAGGGGAATGAAGCCAGAGACCATCTTCACATCAACGATCGCCATGTG11.4492753623188406No Hit
CCCCACAGCAGGGCAGCCACAGGCTGGCACGCGGAGCTCCCCGTGTCTGT11.4492753623188406No Hit
GTTAAAGACCTTCACATACATGTTGAATTAATAGTATTTAATGTCCCTTT11.4492753623188406No Hit
CTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCC11.4492753623188406No Hit
CCATTACTATGGAGAAAGGGAAAATGGGTATTATGGGACAATTAGAAATC11.4492753623188406No Hit
GAAAGTTAAAAGTTTTATTTTATAGTACAGTGATTAGATTGTTAAACTTC11.4492753623188406No Hit
ACCTCCAGTATATACCAAAGCTTTGACTTGTTTGAGCTCTTGAGCTTAGA11.4492753623188406No Hit
TCCTTCCTCTTTGTTTCCACGTCCCAGTCTTCAATAAATTCTATCTGACA11.4492753623188406No Hit
TTTCCACACCATGAATTCACCTATTTTGAGCAAGAATTCAATGAGTTTTA11.4492753623188406No Hit
AAAATGGACACAAATGGACTCCAACCAAGCTCTCCTAGTCTAAGATACAA11.4492753623188406No Hit
AGCCTTCTAAATACTAAAAACCAGAGAAATCTAGCCTTTATGCATGTTTG11.4492753623188406No Hit
CTGTTAGTTGAGAACACACATCGCAAATAAGTTTCTGAGAATGCTTCTGT11.4492753623188406No Hit
GTTGGTATTGTCAACCAATCTTGTCAGTAAAGGCAGTAAAGCCTTATAGG11.4492753623188406No Hit
TTCGTGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGT11.4492753623188406No Hit
GTTTTGAACAAACCGAAAAATGCGCGGCTTTCGATTTCCGAAACGAAAAC11.4492753623188406No Hit
GTACATAGGTACTCAATGAATATTAGCTGTTACTGTTACCATTTATACTT11.4492753623188406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)