FastQCFastQC Report
Fri 10 May 2024
EGAF00003641253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Total Bases8.1 kbp
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCACACGGCGCGGAACGCCTCAGGCAGCCGCCGATGCGCGAAGTCCC33.7037037037037033No Hit
GGATGAAGCTTGCTCTTGACTTTCCTAACATGTTAGGGATCTAAAGTTGC22.4691358024691357No Hit
GACCTTCTCCAGCGTCAGGTCCGATACCGCCTTGCGCAGCCGCGCGTTCT22.4691358024691357No Hit
GTTGTAGGCGGGGTCGGTGTGGTGCCCGTGGCGTTCCCAGTCACTGGCGC22.4691358024691357No Hit
GTACGCCTCTGGCCGTACCACTGGCATCGTGATGGACTCCGGTGACGGGG22.4691358024691357No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT22.4691358024691357No Hit
CACCCATGCCCACCAGTGTTGGTCTTTGCCTCAAAGCCTGAGACCTGCTT22.4691358024691357No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT22.4691358024691357No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC22.4691358024691357No Hit
GTCTATCCCCGCCGCCTGCTTCAGCAGCTCGCCGGTGAGGTGGAGGCTGG22.4691358024691357No Hit
TTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACG22.4691358024691357No Hit
GGGCTTCTTGGCCTTGCCCGGGATGCTCTCGTGCTGCTTGGAGCCAGCAG22.4691358024691357No Hit
AACATTGCCCGGGCGTGCAGACAGCAGGAAGCCCTCTTCCAGCTGCTGAT22.4691358024691357No Hit
TGCCGGTGGCTAGTGGGTCTGGGTTTATTGTGTCGGAAGATGGACTGATC11.2345679012345678No Hit
TTCGAGCCCCGCGCGTACTAGGCAGATAAGAACCCTCCAGCTCGGGGCCT11.2345679012345678No Hit
GTTTACGAATTGGTGAGCCCCGAGTGGTTACCGATTTGTGAACGCGGATT11.2345679012345678No Hit
AGCGCGGCGCGGTGCTCGGCGACGCGGCGGGCGGCGTCGTCGGCGGTGCG11.2345679012345678No Hit
CGATTACCTCGACCACCGCCGCGTCGATCGTCTCGGGTGCGGCGGTCTGG11.2345679012345678No Hit
GCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGG11.2345679012345678No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA11.2345679012345678No Hit
TGAAGACACAGTCTGAGTGCTCCAGGGTGGTGTGGGTGGTCAGGATAGAG11.2345679012345678No Hit
TCTTTGCCATAGTCACGACATGGATGTTTATTCGAAGCTACATGAGCTTC11.2345679012345678No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA11.2345679012345678No Hit
ATACTTTATTCATTAGTGGACTAGTATGTATCCAAAAAGCAATGATCTGC11.2345679012345678No Hit
CTGTAAATCCATTCATTGGTAGATCATGAGCAACAGTCACTTAATGTCTC11.2345679012345678No Hit
GTTCAGATGCATAGGCCTCAGGCGGTTCAAGGCAGTCTTAGAGCCACAGA11.2345679012345678No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT11.2345679012345678No Hit
TGTATTTATTTACCAATCTTGTATTTATCATGGCATAAACGATTATAATT11.2345679012345678No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT11.2345679012345678No Hit
AGACGGGGAGAAGGTAGAGCTGGGAATTCATTTTTCTCCCTACAAATCTT11.2345679012345678No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.2345679012345678Clontech Universal Primer Mix Long (96% over 26bp)
GGGACTATCACGTCTACGCCATGACGCCCGACTGCCCGAACTGGCGTGCC11.2345679012345678No Hit
CAGGAAGATCACGCCCAGCAGCACGGTGAGCCACATGAAGCGGATGGTCT11.2345679012345678No Hit
CCGTGTCGTCGTTCGATTTCAGCATGGGCCCGCCCGCGCTTTTCGCGACG11.2345679012345678No Hit
GGCAGTGCCTGCATCAGCAGCGGGGTGGCCATGCGCATCTTGCTCACAGG11.2345679012345678No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC11.2345679012345678No Hit
ACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC11.2345679012345678No Hit
GTCGATGGGGAGATTTAGTAGCTCTGTGAGCCTCCCACCCAAAGACCACG11.2345679012345678No Hit
GAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGTGTA11.2345679012345678No Hit
CCATAAGATAGTAAAAACTGCAGATAGGGTAAATGCCAGTATTGGAAATA11.2345679012345678No Hit
GTCCGGCCACAGCCTAACGCTCTTCGCTGTCGTTTGTGGTCTCGCGCAGG11.2345679012345678No Hit
GTGTGGTGGAAAGAGCCGGGGCTTTGGAGTTGTCATCAGAGAATATTGTG11.2345679012345678No Hit
CTCTAGGACCAGATGAACTTGATGAAGTGGTCAAATCTGAACTTGAAGTA11.2345679012345678No Hit
GAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGA11.2345679012345678No Hit
CGACAGCTTCAACCGGCCCTTTGCGCTTGATCTCGCCAGCCGGATGCGCG11.2345679012345678No Hit
CTACAGGGGCTCCCCTGGGTGCGTCATGGATGCTGTTCTGCGCCACGGCT11.2345679012345678No Hit
GCCTCGGACAACAGCGCCTCCGCCTCGTCGAGATCGCCTTGCTGATAGGC11.2345679012345678No Hit
GTTACACGTTTGGCATGGATAGCACACAGGTTGGTGTCTTCAAAAAGGCC11.2345679012345678No Hit
TAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCC11.2345679012345678No Hit
GCGGGATCACCGCGCCGGCGCAGAAGCCCGACGCGGCGCGCAGCGCCATC11.2345679012345678No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.2345679012345678No Hit
GTCTTTTTCTTCATTCCACCTCGTGGAGAAGACAATTTGAAGGGCCACAG11.2345679012345678No Hit
GCTTACGGTTAGAAAGATGGCGTTACCGTTGGCGGGAAGGTAGGTGAAAC11.2345679012345678No Hit
GCACTGGGGGAGAACCGCCGATGATCGACAAATCGCCTGTGGGTTTGATC11.2345679012345678No Hit
GAATAATATGATTACTTGTAGGGGTTTCCAAATAGTTATGTATGATTTCT11.2345679012345678No Hit
TTTGCCAGCGCGGCGCGCTTGCTGAAGGAAAGCGGTGCGGCCGCGGTAAA11.2345679012345678No Hit
TCCTGGGTTCAAGTGATTCTCTGGCCTCAGCCTCCTGAGTAGCTGCGATT11.2345679012345678No Hit
CTCTGGGGCTGCGGGGTCAGCAGAAGACTGGCAGTGCTCCTCGGGCACGT11.2345679012345678No Hit
CGAAACAACCTGGTGAAGTCTCGCAAGGCCCAGCAAACTTGTTTACATTC11.2345679012345678No Hit
CCACAATCTTTATAATAAAAAGTAGACAGACAAATGAGTAATTTGCTACA11.2345679012345678No Hit
GGGCCCCCCTCTCACCCCTTCCTCCCTGCACAGCTCTGGCTCTTCCTGGA11.2345679012345678No Hit
TCTCGTGTGCGACCCCTTCGCCGGCAGCGGGGTCGTGCCGGTCGAGGCCC11.2345679012345678No Hit
GCATCGAGGTCGTAGTGCACCCAGACCAGGTAGGCACCGATGCCGACGCC11.2345679012345678No Hit
GTACATGGGAAGCCAGCCTGGCCAGCTGCTCAGACCAGCTTGCAGAGCCG11.2345679012345678No Hit
TCACTGTCGTCCCACCCCACTTCTTCCACCCCACTTCTTCCTTCACCAAC11.2345679012345678No Hit
GCTTGGTCCACACCGATGCGGCCGCCGCCACGCTCACCTGCTGGCCGACG11.2345679012345678No Hit
CAATTACACTAAGCTTCAGAAATTGTTGGATTTGTTTAATTGAGTGGTGG11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)