FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length100
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACC42.547770700636943No Hit
GTTATGACCCACGTTGTCACCGAATCGTGCATCGCATGTCGCTACACTGA42.547770700636943No Hit
CCCAGACCTGGTGGGCCCCGGCACCCATTTATGCCGAAGCTGTCTCTTAT31.910828025477707No Hit
GGGTCGCACTCGGCGTCGTGATCGCCGATCTGGAACTGGGTCATCATGTC31.910828025477707No Hit
CTCCTGCGACTCGCTCATCAGGATCTCGGCGGGCGTCAGTGTGCTGTCGC31.910828025477707No Hit
TCCTTCGTGAAGCCCTGCTTGCGCACGGTCAGGTGCACAGCGACCATCAG31.910828025477707No Hit
CAGCGTGCCGCTGATCGGCTCGCTCATTACCGAACCGGCCGCGATGACCC21.2738853503184715No Hit
TACCAGCAGGTCGCGCCGGAGTAGTCGGTGCGCGCCTTCGGCCAGAGCGC21.2738853503184715No Hit
GATCAAGGCCGCGGACGTGGACCCGAAAGTACACCCGAAGAACTACGACC21.2738853503184715No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC21.2738853503184715No Hit
CCCGGACCCTCGCCGCGGGGTGCGGAGATGACCGCCACCGCGGCGCCACC21.2738853503184715No Hit
GTCGTCGTCGCCCCCTCGACACACCGGGCCCGGCAGGTGGTGCAGACCGA21.2738853503184715No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.2738853503184715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCAGGGACGCACACCCGGCCACGGGCGGAGGGATCCGGGCCCGGATCG21.2738853503184715No Hit
GTTCATCGAGAACATCACGCACGCTGGATCACTCCTGTCAGCTCCTCGAA21.2738853503184715No Hit
GACCGTGGCCCGTACGTCGCCGGGCGTTGCGTCGACCTGTCGCGTGCTTC21.2738853503184715No Hit
GCATGCTGCCGCGTGAAGGCGGTTTCAGCAGCCAGATGAATTCGGTCACC21.2738853503184715No Hit
GTGCCGAGCATGCCGAGCTTGGTGGCGCCCACCGGCACGTCGTCGAGCAA21.2738853503184715No Hit
GCGAGATCGCGGGCGCCCTCCACCGGCTGGGCGAGCGAGGCATGACCGAC21.2738853503184715No Hit
ACCTGGGGGTCACGGGCACGCCGACCTTCTTCCTCAACGGAGAGAAGCTC21.2738853503184715No Hit
CATCGCCGCCGAGCTCGCCGACGCCGTCGTCGTCACCGACTTCAACCCGC21.2738853503184715No Hit
GACCGTGATCGTCCACCGGCTGTCGACCAGGTCGTCGACGAGCAGGATCG21.2738853503184715No Hit
CCGTCGGGCAGCGCGAGCAGCTCGATCTCGGCGCCCTCGTGCTCGAAGCG21.2738853503184715No Hit
GCGCGGTGTAGAAGGTGCGCTTGCCCTGCGCGAGCGCCGCGAAGTGCGCG21.2738853503184715No Hit
CCGCCCGGGTCGCCGCCGGGGTCGTCGGGCGTCGCCTCGATCGGCGGCGG21.2738853503184715No Hit
CCGCAACAGCGGCCGGCGCGAGAAGAACCTGTGGACCGCCGCCGGAGACG21.2738853503184715No Hit
GTGCAGGCGCCGAAGGGCGAGTTGAACGAGAAGGACCGCGGCTCCAGCGC21.2738853503184715No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC21.2738853503184715Clontech Universal Primer Mix Long (96% over 26bp)
AACAAGTTGAAGTACCTGGCTTTCCTCCGCAAGCGGATGAACACCAACCC21.2738853503184715No Hit
CGGTAGGTGGTCTCGATCTGGTACCCGTAGCCGTTCGCCTCGAAGCTGTC21.2738853503184715No Hit
GCTCTGGAAGGCGTGAGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG21.2738853503184715No Hit
CGGCTGGGCTACGAGCAGCCGGTCGACATCCTCGGGTTCTCCTGGGGCGG10.6369426751592357No Hit
GGCGGATCCCGTGCGCCTGCAACTTGTCCTTGCCTCCCTGCTCTCTGTCC10.6369426751592357No Hit
GTCCTGCGTGCACCATGGTGGTCTCCTCGATCTCAGGTCAGGCCGCGGCG10.6369426751592357No Hit
GGACCGACTCATCGACGTCCCGCTCACCGAGACCAACGGCAAACGCGGCG10.6369426751592357No Hit
GGATGTGGTCGATGCCGCCGGCCGAGCCGCCGGTGACGGCGACGCCCTGG10.6369426751592357No Hit
ACCCTGACACGGGGATCATCAGCATCCGCAGGTGTCGGACCTGCGGATGC10.6369426751592357No Hit
GGACAGGGCGGACTCAATTGCTGACGGGTCTCATTTCGAGCTGCTCATCA10.6369426751592357No Hit
CTGTGGCTGTGGCCCGCTCAGCCGCCGGTAGACTCGCCACTCGTACGGGA10.6369426751592357No Hit
CCTCGTGCAGGTCCAGGAGCTTGTGGTCGACGACCCCGTCGAGCAGCTGG10.6369426751592357No Hit
CACTGAGAGAAAAAGCTGCAGCAGAGGCCTGCTGGAGAACAGTGGTCGGA10.6369426751592357No Hit
GAGAAAAGGTATCTGTGGGAAGAGAATAATCTCTTTTGACTTCGTGTGCT10.6369426751592357No Hit
GCCTTCGCCGTGCAGGACGACTCCGACTTCCCGGTCGGCCCGACGATCCA10.6369426751592357No Hit
GCCCGCAATCGTGATTATTTAGCTCATGAGACAAGCACCAATATTAAAAA10.6369426751592357No Hit
TTCCAGGACATCACCTGGGACGAGGCGCTGGACAAGATCGCCCGCGCGAT10.6369426751592357No Hit
TTCTTCACCCCACCCATTCACAGGCATTAGCTGTTCTTTCTCGTAGCCCT10.6369426751592357No Hit
GGCCGACCGCCCCCGCAAACCCCGCGCCGGATGCCTCAGTCATCGGCGAG10.6369426751592357No Hit
TATCAACGCAGAGTACATGGGATCCCCTCGACCGCTCGCGTCGCATTTGG10.6369426751592357No Hit
AGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTT10.6369426751592357No Hit
AAGCAGTGGTATCAACGCAGAGTAGCTTCCCATGTACTCTGCGTTGATAC10.6369426751592357Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTCGCCGCCGTGCGCGTCGAGGTGCCGGAGGTGTTCGAGGCGACGCACG10.6369426751592357No Hit
GTCGTCACGACGGCGAAGTCGGATGACGAGGGTCGCGCGCTGCTGAAGGC10.6369426751592357No Hit
GTCGCGCAGCCGTGCCGCGACCTCGAACTGCAGCTCCCGGGCTGCGGAGA10.6369426751592357No Hit
GGCCTGGACGCGCAAGGCGGCAAGCGAGGCAGCGCTGGCGGCACGCGGCA10.6369426751592357No Hit
GCACCGTGAGGCGAAGCGGCGCCCCGGGGCCGGGTTCCAGGCAGAGGGCC10.6369426751592357No Hit
GCTCATGAGCGGCACGGTGACCGTCTCGGCGGCACCGAGGCCGGCGAGGG10.6369426751592357No Hit
CGTCCGGGCCGCCGGGGATGGGCTCGCCCCACACCTGCTGGCAGGTGAGG10.6369426751592357No Hit
GGTCATGAGTCCTTCCACGATACCAAAGTTGTCATGGATGACCTTGGCCA10.6369426751592357No Hit
GAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAG10.6369426751592357No Hit
ACGCCCCGTCCGCCGATCGCCACGCGGCCGTGCTCGCCCTCAGCGACGTG10.6369426751592357No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10.6369426751592357No Hit
GCCCCAGATGCCCACGCCCCGGACGATGTTCACCCCGGGGGTCTCGGTCG10.6369426751592357No Hit
CTCGAGGTGCTCGCCGGCGGCGGTCCGGCCGACGTCGTCGAGCTGACCCT10.6369426751592357No Hit
GCCTTCTGGAGAGGAGCAGAGATACACGTGCCATGTGCAGCATGAGGGGC10.6369426751592357No Hit
GCCTTCTCGAGAGGAGCAGAGATACACGTGCCATGTGCAGCATGAGGGGC10.6369426751592357No Hit
GCCGGCGACGAGCAGCACCGCGGCTGGACGAGCGGCGCGCGCGTGATCGG10.6369426751592357No Hit
GCTTCGTCCTGGCGCAGCAGGAGCACGCCACCACGTCCCGAGCCATCCAT10.6369426751592357No Hit
CCAGCGAGGACGTCCGGCAGGCGATCGAGGACCTCGGCGTCCGGTACGTC10.6369426751592357No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG10.6369426751592357No Hit
CACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAG10.6369426751592357No Hit
TTGGTGAGTTGCGCGTCCATCCCGGCGGCCGGAGTGCCGGGGCGCTTGGA10.6369426751592357No Hit
GCTGGGCGCAGTGGCTCCTGCCCATGATTTCAGCACTTTGTGGGACTGGG10.6369426751592357No Hit
GCTCTCCCTCGCCGTCGGCGACGTCCTCCTGCTGCGCCACCCGCGGGACA10.6369426751592357No Hit
GTGCTCATCCACGGCAACGTGTCGTCGTCGCTCTTCTTCCAGCCGCTCAT10.6369426751592357No Hit
GCTCAGCTTGCAGCGAAGCGTGCCGCACAATTGATTCAGAGAATGCGCCA10.6369426751592357No Hit
TTGATGAGCCGCACCAGCGTGGACTTGCCGGCACCGGAGTAGCCGATGAC10.6369426751592357No Hit
AGGTACCAAAATGTCCGATGGCGATGCCTTCGCCGCCGGTGACCTTTCGT10.6369426751592357No Hit
CGTGGTTGCCCACGCTGGCGTAGTCCAGCCCGGCCAGGCCGAGCGACTCG10.6369426751592357No Hit
TCAGAGCGTCGTCCTCACCGAAGAGGAACTGCGCGAGCGCCTTGGCCAGT10.6369426751592357No Hit
GCCCTGCGCGCCGCGTCGCTCGATCCAGCTGCCGTCGAGCGGGTCCTCCG10.6369426751592357No Hit
GCCCCGCGAACCAGGCGCCGCCCGCGACGATCTCGCCGCCGGTGAGGGCG10.6369426751592357No Hit
GGGCGGGCAGAGCGCCCGGGCGACCGCGCGGTTCTTCGACCTCACGGCCA10.6369426751592357No Hit
CACTGGAGCAGGGATTGGGCCCCCAAGGCCTCAGGTAGCCCCGTGCCTGT10.6369426751592357No Hit
GTCCCGACCATGGTCGCGGGTTTTCGCGCAAATCCAGTGCTTGCGTTGAG10.6369426751592357No Hit
CGCCTCCAGGTCGGTTTTCTTGGCCGTGCTCCAGGTCGAGAACGGGGTGA10.6369426751592357No Hit
CCATCGGCGCCGGGGACAGCGTGCTCGCCTCGCTCGCGGCCTCGGTGCTC10.6369426751592357No Hit
GGTTTACACACCCGCGGGATGCTGGAGTCAGCGCCGCACAGAGCACTGCG10.6369426751592357No Hit
CCGTCGCCGCCGCGCACGACGGCAGCCTGGCGCCCGTCGTCGACAGCCTG10.6369426751592357No Hit
GACCCGGCGTGGGCGGATGCCGCGTTCCCCGCCCGCGCGACCGAGCGCAT10.6369426751592357No Hit
GGACAGCCTCGAGGAAGCCGGCGACGCTCTGTTTGCGTTCACCAAATTGC10.6369426751592357No Hit
ACCGCGGCCTGCGGGCGGCCGGCTTCGGGCGCGAGTGGCAGCTGCCCTGG10.6369426751592357No Hit
CATGAGCAGCGGGAAGTTCCACGGGATGACCTGCCCGGCGACGCCGAGCG10.6369426751592357No Hit
CCGCATGAGCGACTCGGGGAGGAGGTCGACACGGTGATCGTCCTCAACGA10.6369426751592357No Hit
GTGGTGCCCCAGTGACCGAGCAGCCGCGGCTTGATGTGCTCCGGAAGCAG10.6369426751592357No Hit
ATCGTGAACAACGACTTCCTCGGCCCCGCCGGGCTGTCGTCGCAGGACGC10.6369426751592357No Hit
CATCAGCCTTGACCAAGCCATGTCCCACCCGGACTGGATCGGCACCCCTG10.6369426751592357No Hit
ATCTGGGGCCGGCCGACATCGGACCACGTCGAGGTCCGCTACGAGAACGT10.6369426751592357No Hit
GCGCGAGACGATCCTGCGGATCGAGCGGTGGGACGAGCATCACACGGATG10.6369426751592357No Hit
GCCGACGACCGAGCCCTTGACGGGGTGGGCGGTGACGTTGACGGGCAGGG10.6369426751592357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
GGTGGCGATATAAGGGTGGGTTTTCGCATCCACGTTCGGATTCACCCGCA10.6369426751592357No Hit
CATGACGCCCCCCGAAATCGTCTCGGAACTGGACAAGCACGTGGTCGGCC10.6369426751592357No Hit
AAGCTCAACGAAACGCCTCCATCGCTTCCGCAAGGGCCTGTCTCTTATAC10.6369426751592357No Hit
CCCACAGCCCAGGTCGCTGCTTGCTGCGGAACGCACTGACCGTCGAGGTC10.6369426751592357No Hit
CCACGAGGCGGTCGGCCTGGGGGACATGGGCAACCCGACCGGTTTCCTCG10.6369426751592357No Hit
GTGCGCATCGACGAGGACGTCCAGCAGCAGATCGAGGACCTCGCCCCGCT10.6369426751592357No Hit
ACGAGGACCTGACCGGCGGTGCGCTCGTCGCCGGGACCGGGACCATCGAC10.6369426751592357No Hit
TCGTCAGCCAGGTCGTCGAGTGCAACGGCATCGGCGAGGGCGGGCGCCCA10.6369426751592357No Hit
GAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAA10.6369426751592357No Hit
ATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG10.6369426751592357No Hit
CCGCTCCACGGTCCGGTGCGCGTTGCGGTAGTGCCCGCGGCGGGTCAGGT10.6369426751592357No Hit
CTGCGGCACACGGCGGCGAGCTTGTGGCTGGCGGCGGGGATCGAGCCCTA10.6369426751592357No Hit
GATCCGACCACCGCTCGCGTTCGACGACGCGGACACCGACCGCTTCCTCG10.6369426751592357No Hit
CGACCAGCTCAACCGCAACGAGACGACCAATGCGCTGCGCAGCGCGCGCG10.6369426751592357No Hit
GACGCGGATTGGTCCCTGCTTGGCGCGTTCGGCGAACGAGGCGATGACAG10.6369426751592357No Hit
GCGTTGACGGGTGCGGAGGCGGGCGGATGCGCTGGATCGGGAGATCCTCG10.6369426751592357No Hit
TTGCAGAATCCATGCGATCGCCAATCGGACACAGCCCAATTTTATGTTCG10.6369426751592357No Hit
ACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGC10.6369426751592357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)