FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA38.571428571428571No Hit
GTTTGAGCATTTTCTCGGCAAGGTTGCTTACCGACGAGTCATCGGTATCT38.571428571428571No Hit
CGTTTTTCAGGACCTGTTGGTACCGATCGGAAGAGCGTCGTGAGGAAGGT25.714285714285714No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA25.714285714285714No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25.714285714285714No Hit
GGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCA25.714285714285714No Hit
CCTACAGGAAGCCTGGCTGAGCGCTACTCCGTCATCCTGCTCGACACGCT12.857142857142857No Hit
ATTCTATCATTTGAATTTGTGATTCTTCAGCAAATGGAACAAGATGGTAT12.857142857142857No Hit
CCGGCCCAACGGTGATTACTCCAAATGATTCCTGACCGGTTACCGGATTC12.857142857142857No Hit
GAATTCACTTGTGGCTATTAGAGCTGGAGGCACCCTTAGCCACTTCATTC12.857142857142857No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA12.857142857142857Clontech Universal Primer Mix Long (95% over 21bp)
TCTATGACCTCGGTACTCGGGAATAGCCACATTAAGGCGTTCTGCACCTG12.857142857142857No Hit
CTTCTGTTTCTTCCTTAATGATGGATTCTGACCCTTCACCTGCAGTTTCT12.857142857142857No Hit
CCCCCATCCCAGCTTCTCCACCATCCCAGCAAGTCAGGATATCAGACAGT12.857142857142857No Hit
TCACTCCTCTGTTGCCATTTTTAGCAAGTACTCCTTGTCGATCTTGAACA12.857142857142857No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA12.857142857142857No Hit
GTGTCAACCGCTGCATTCCTCAGTTCTGGAGGTGCGATGGCCAAGTGGAC12.857142857142857No Hit
CGGCAGCCCTCGTCCTTGAGCTTCTTGAGCAGCAGCCCGAGCAACGACTC12.857142857142857No Hit
ACGCAGAGTACATGGGTAGGGCACGAGTCACACACTTCCCTACACGACGC12.857142857142857No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC12.857142857142857No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGGTTAGACCG12.857142857142857No Hit
CACCTACCCTTATCGTCATCCCCGTCATTCAGTCGATGAAGCACACCCTC12.857142857142857No Hit
GAGATTAGGGACACTTCTTCCTTGGGGATCAGAAAAAAAAGTATCAACGC12.857142857142857No Hit
GATCATAGGTTCCAGTAATGGACAGTTTGGGTAAATGTAAGCTGGCAGAC12.857142857142857No Hit
GTTCCCGTAACCTCCGCCTGAACGGCGGCGGCTGGGTATTCGACGTTGGC12.857142857142857No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG12.857142857142857No Hit
ATCATAGGCATGTAGATGGCCACTCGGTCCCCCTTCTGAATGCCCTGTTT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)