FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA511.627906976744185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA24.651162790697675Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCG24.651162790697675Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24.651162790697675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24.651162790697675Clontech Universal Primer Mix Long (96% over 26bp)
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC24.651162790697675No Hit
GGCCTACACAGGGTTCAATATCGCCGCACAACGGATATCGATACGCCCTA24.651162790697675No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAGTCGCGCCCAGCAACCTCGGCGCGCAGGTCATTGCCAACCGG12.3255813953488373No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GTGGAAACAACGCAGAGAACATGGGAAGCAGTGGTAAAAACGCAGAGTAC12.3255813953488373No Hit
GCGATCGTGTTGACGATGTCTTCGACCAGCGCACCGCGCGACAGGTCGTT12.3255813953488373No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.3255813953488373Clontech SMART CDS Primer II A (96% over 26bp)
GTCGCTGACTGCGACCACGGCCGCCAACACGTCATGTGCTGGCGGTTTTT12.3255813953488373No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGATTCTG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACCGGTCGCTTATACACATCATACG12.3255813953488373Clontech Universal Primer Mix Long (95% over 21bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GTGATACGACGAACGGTCTCGCTCAATCCGGTGACGGAGCACGCCCGAGA12.3255813953488373No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATTTT12.3255813953488373Illumina PCR Primer Index 6 (96% over 27bp)
TTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGA12.3255813953488373No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12.3255813953488373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC12.3255813953488373No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA12.3255813953488373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGACGGCGGGCGCGTGGAGATCGACGGGCGCGACCCCTTCGCCGACCGC12.3255813953488373No Hit
GAAAGAACCGGACGTGAAACGCGAAGGCCGCCGGTGCGGCTAGCTGGCCC12.3255813953488373No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
AGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAA12.3255813953488373No Hit
CGCACACACACACACACACACACACACACACACACACACACACACACACA12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)