FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003641443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTG27.4074074074074066No Hit
TTACAAAGTTAAACAGCTAAAAGAAGTAAAATAAGCTGTCTCTTATACAC27.4074074074074066No Hit
ATCCAGCAGTGGCCTGGACTGCACTCACATAAAAATCATGAGACAGCCAT27.4074074074074066No Hit
AGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTC27.4074074074074066No Hit
TATCAACGCAGAGTACATGGGATGCGTGGGTGAGGCGAGGGCTAAACGGG27.4074074074074066No Hit
ACCATGTCCCATCACTGGGGGTACGGCAAACACAACGGACCTGAGCACTG13.7037037037037033No Hit
GGATGAGTCTGCAGTGGACTGCAGTTGCCACCTTCCTCTATGCGGAGGTC13.7037037037037033No Hit
CACATTCAGATGCCTTTGCAGAAAGAGATGCCAGAGGCTCTTGAAGTCAC13.7037037037037033No Hit
GCTTGAGACCATCAAGGGAATGTTTAGTTTCCTCCACAAAGAGCCACCAG13.7037037037037033No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA13.7037037037037033No Hit
GCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGGCCTGGAGGCTATCCA13.7037037037037033No Hit
TACTTATCCCGCGCCTTCACCACAGCACCAACTATGCCACTCCCACATTT13.7037037037037033No Hit
GTCTCTGAGACAGTTCATCCTGCTCCGCCGAAGCCACGCTCGTGCCCACG13.7037037037037033No Hit
GTCCCCTGCCTCAAGATTCTCCTCAGCAAAGGCCTGGGGCTGGGCATTGT13.7037037037037033No Hit
GAGGGAATGGATATGAACTACATTATCCAAAGGAAGAAAGAATTTCGGAA13.7037037037037033No Hit
GTGGATGCACTGAAGAAGATGTTGTTGGATCAGGGGGGCTTTGCCCCGTG13.7037037037037033No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAG13.7037037037037033No Hit
GTGCTAACATGCCTTGGTTCAAGGGATGGAAAGTCACCCGTAAGGATGGC13.7037037037037033No Hit
GAACTAGGGTCCTTCAGACTCCAGATGCCCTGACCTGGGCCTTAGGACAT13.7037037037037033No Hit
GCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT13.7037037037037033No Hit
ATCGAGACATGTAAGCAGCATCATGGAGGTTTGAAGATGCCGCATTTGGA13.7037037037037033No Hit
GGCGATGTAGCGGGTGATGTACTCCACAGTGACCGGGTCCTCCACAGTCA13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)