FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGTCTTGTTGCGGGCACCGCAGTCGCCGTGAAGATGGCGTCTACCA410.0No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT25.0Clontech Universal Primer Mix Long (96% over 25bp)
AGCGTCTCCTTCCCGTTCTCCAGGTATCTGCGGAGCCACTCCACGCACGT12.5No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC12.5No Hit
CGTCAATCATGTAGAACGAATCATCAATGAAGATGATGAAGAGTTTTTGC12.5No Hit
GTACATGGGAAGCAGTGCTATCAACGCAGAGTACATGGGTGGTATCAACG12.5Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCATGGGGGAGTGCAGCAGCTCCAGGAGCAGGCCGATGGCTTGTGCCTC12.5No Hit
ATCATGATCTCCGGTGACAGCCGGCCCGTCGCACAGGCCGTCGCGCGGGA12.5No Hit
GGGTTGGGCCAGTGATAACCCCCAGCCGCAATGACGGAATACCACCTTCC12.5No Hit
AGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTAC12.5No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA12.5Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTA12.5No Hit
TACCAGCACCATGCTATTTTGGGTACTGTAGCCTTATAGTATAGTTTGAA12.5No Hit
GACGAAGAAGGCGTCGACGCCGAACGATCCGCCGGTGAAGTCCTGCCGGT12.5No Hit
CCGCAACGCCTTCATGGCGACCCTCGTGCACGATTTGAAAACCCCCCTCA12.5No Hit
GTATTCCAACCCTCCTGTGCAGGGAGAAGTGATGGAGGGTGCTGACAACC12.5No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG12.5Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAAGCCATGTCCTCATTGAGTTTATAAATTTTTTCAGAAAAAAAGAC12.5No Hit
GTTGAAGGTGAAGTGATGACACTTCAACGCCGTCCGGGAGCCAAAGTCCA12.5No Hit
GTGCTGGGGCCGCGCGCGGGCCAGGGCGTTCACGTGGTCAGCCGTGCGCT12.5No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTGTTACCACTGGTTCCCATGTGCC12.5No Hit
GGTCAGATTCCACGATGGTTCATCGATTGAAAAATTTATTTCCTGGGCCA12.5No Hit
GCCTGAGGGAGTCGCTGACGGGCACGCTGACTGGAGGCTGGCGGACAGGC12.5No Hit
GCTCAGCAGAAAGTAGGCTGGGCTCTGGCCATCCTTTCCTGCCTCTGTGC12.5No Hit
ACGTAGAACGCGATCACGAGCCACACCGCCACCAGCCCCTCGTCGGAGCG12.5No Hit
TTTCTGGAATCTGTGAATACGAGATATCACTCCCATAATTATATTATGCT12.5No Hit
TTCTGTGGGTGTAGGATTCTCCAGGTTCTCCAGGCTGTAGGGCCCAGAGG12.5No Hit
CTGCAGGGTCTCGATCATCTTGCGGTAGCGGACCTTGGCGGTCTCGCGCT12.5No Hit
GTGAAGGAGGGGATGGCATAGCATTATGTGTAAAAAACAAGTATCTGTAT12.5No Hit
TTCACACAGAGGCACCAAGGTTTAGTGACTTGTCTACTGTCTCTTATACA12.5No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC12.5No Hit
TTTGGGAGGTATAAACATCCTCCCATTTTCCTTCTGATTATGGTTCTTTT12.5No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTATTGCTTGTATCTCGTATG12.5No Hit
GTGCAAGGTGTACGACTCGCTGCTGGCACTGCCGCAGGACCTGCAGGCGG12.5No Hit
CGGTAAAAAATCAGTAGAGCAAAAGCGGTAAAGGATGAGGTCTGTCTCTT12.5No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)