FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCTTCTGTTCATATACTTGCTTGAATAAATTATTTGTTCGATCAACT410.0No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTGCGCTGCATGT12.5No Hit
GCAGCAAGGTGGGCTCACTACAGCAAGGGGCAGAGCCACCACCACGGTGC12.5No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG12.5No Hit
GTACATGGGGGAATACAGCTAACCTAGAAGGTGAACAATCTCTATAAGGA12.5No Hit
GCCCCGCCCTGCTCAGCCTCGGGAGCGGACGAATTCTCAGCTGGTGGATC12.5No Hit
CTATTGGACATGCAGGCACCTGTTTGGGAATTTTAGCAAATGATGGTGTT12.5No Hit
GAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT12.5Clontech SMART CDS Primer II A (100% over 26bp)
CCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACG12.5No Hit
GTCTCGAGCGCGACGTCCGAGCCCGCAGCGCCCATGGCGATGCCCACGGT12.5No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG12.5No Hit
GGAATATTGGGACCGGAACACACAGATCTGCAAGACCAACACACAGACTG12.5No Hit
ACACAGTACCTAAATGGGTAATTTGCTCGACATGACCGAGTAAATTTAAA12.5No Hit
ATATTTCGATTGCTTGGGAAGTTATCACCATCTTAAACCCTTCTTTGTAC12.5No Hit
GAAGGAGGGTGAGGATCAAAAAACTACCTATCTGGTACTATGCTTTTTAT12.5No Hit
TAAGCGAGCTTTTAAGGCTTCGATTCGTGCGAGACTATCACCTGTTCCGA12.5No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC12.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACAAAGCAGAGAGGAACAGATTGGAGCCGTGGCACCTTAAACGTAGTT12.5No Hit
CCCGTGGACCGTGGCCACCGCGAAGAGCGAGGTGTTGGTGTAGGAGACCG12.5No Hit
ATCAAAGACAACATATGTACAAACATTTTACAAAAAAGAACATTACCAAT12.5No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAATTACC12.5Clontech Universal Primer Mix Long (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.5No Hit
TAGACAAGTCACTAAACCTTGGTGCCTCTGTGTGAACTGTCTCTTATACA12.5No Hit
GCGTGCGCAGGGCGAGGTCGTCGGCGAAGGCGCGCAAATCGACGTCCGCC12.5No Hit
AGATAATATAGAGCTTGATTCAATTAGTAAAACAATTAGCTTTGTAAAAT12.5No Hit
GTTGTACTTTAAATCTCTGTGACTTTTCCTTCTGTCGCCAACTGGTGAAA12.5No Hit
TGCTTGTCCCTTTTGATCGTGGTGATTTAGAGGGTGAACTCACTGGAACG12.5No Hit
AGGGACAGATGATCCAGAAAGCGTTCGAGAAGCTGCCGCTCCTCTTCTTG12.5No Hit
TCCTTGGTCCCTTCCTTCAGGAGCTCCGAGAGGTCAGCCATTCTCATTTG12.5No Hit
GTCATGAGGATGTGGACCGACGTCGGTGGTTCATCACCATCGGCATCGGT12.5No Hit
GTCTACGGCGGCGAGCTCGGCGCCGGGCGCATCCTGCTGGGCGCCAGCGC12.5No Hit
GTGTGACCTCATGCATGACCCCGAGTGTATCTACAAGGCCATGAACATAG12.5No Hit
ACCTCATCCTTTACCGCTTTTGCTCTACTGATTTTTTACCGCTGTCTCTT12.5No Hit
GCCGAGATCTGTTCCAAACCCTCGGCGAGCTTTGCCGAGACGCACGAGGC12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)