FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA167.2727272727272725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT104.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG94.090909090909091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC83.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC73.1818181818181817Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA62.727272727272727Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62.727272727272727Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31.3636363636363635Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.3636363636363635No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA31.3636363636363635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAG20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
GCTCCAGGCAGTCCACACACTCCTCCATGAACCACTGCACGAACTGGGTG20.9090909090909091No Hit
GTACATCTTTGCTGTCTTTCCATCCAGCTCAGGAACTGCAATTTCTGGGG20.9090909090909091No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.9090909090909091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACG20.9090909090909091No Hit
ATTCATTACATTAGCCAAGAATGGGTGTGGACGTGAACATTCTGGAAGGG20.9090909090909091No Hit
GTCATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.9090909090909091Clontech Universal Primer Mix Long (100% over 23bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATC20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
CGCCTTTTCATCAATCGCCCACATCACTCGAGACGTAAATTATGGCTGAA20.9090909090909091No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
CAATGCCACCGTAGATAAGTTGGAGGACTTCATCAACAACATTAACAGTG20.9090909090909091No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATATCACTGCTTCCCATGTACTCTGCGT20.9090909090909091Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.9090909090909091No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTAC10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCCAGTGCCATCTCTGAGCTGGTCAGCACAGCCTGCGGTTTCCGGCTGCA10.45454545454545453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCA10.45454545454545453No Hit
CGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGTTTAAAAAAAAAAA10.45454545454545453No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAAGGG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAGTTTTGGCAGCAGCGTCCAGTGCCCTGCCAGTAGCTCCT10.45454545454545453No Hit
GGTATCAACGCAAAAAAAAAAAAAACAAAAAAAAAAAAAAATTAAAATGG10.45454545454545453No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCC10.45454545454545453Clontech SMART CDS Primer II A (100% over 26bp)
ATGCCGATGTACGCCGCCTGGGCCTGTCGGAATACTTCCGCTTTGCCCTG10.45454545454545453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAA10.45454545454545453No Hit
GTTTTGGGAGCCCGCCGGTGAGGCCGGGCCACGCTCAGACACTTCGATCG10.45454545454545453No Hit
CCTTAGAGGCTCTGGAATGCCCCCCACCCATCCACCCACCAATGGAAGGA10.45454545454545453No Hit
GCCCAAAATGAAGAAATACAAGAAATGGCTCAGAATAAGTTCATCATGCT10.45454545454545453No Hit
GTATCAACGCAGAGTACATGGGCCTTTTCCCACCCCCTAGCGCCGCTGGG10.45454545454545453No Hit
GAGTACATGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGTAGAGACGCAG10.45454545454545453No Hit
TGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCA10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGCACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTGATACCACTG10.45454545454545453Clontech SMART CDS Primer II A (95% over 23bp)
GACTTCACGCGGTGTTTGACAGCGCAGCTCCAACTATCGCACCGCTCCGC10.45454545454545453No Hit
GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGTCTCTT10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTTCAGAAAATCCTCAGCAAAACTGGAAGTGCCCTGCCTCTGAGGTGC10.45454545454545453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATAACACTGCTTCCCAT10.45454545454545453No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGTAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAG10.45454545454545453No Hit
CATGAGCGGCATTGCCATCCAGTCGCGCCAGTTCGCCGCCCAGCAGGCGC10.45454545454545453No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAAACAA10.45454545454545453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATACACATCTC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GGGTGTGGGTAGCGGACAGATGGGACGTAATGGCCAAGAAAGAGGGAGCA10.45454545454545453No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA10.45454545454545453No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTTTAAACG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ATATAACTAAAGTGTTAGTGGATTGGATTAAAAGAAACTTATTAGGCAAG10.45454545454545453No Hit
CACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCC10.45454545454545453No Hit
GTCCAGTTGCTCCCAGTGACTGCAGAGTAACCACAAGGCTGCCCGAGGCA10.45454545454545453No Hit
TTTTTTTGCAATGGTTGCAAGTTCAGTGGCAACCGCTTGGGTGTCCAGAC10.45454545454545453No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTGGGAAGCAGTGGT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCAACGCCGAG10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCAAGCAGTGGTATCAACGCAGAGTACATGCAAGCAGTGGTATCAAC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
CCCAGATGCAGTCCATGACTAAGATCTACCATGGACCCCTGGACCGGCCT10.45454545454545453No Hit
GAAGCGCTCAGAACCCTTCACAAGATATGGGATGGACCTTTCGGCCAGCG10.45454545454545453No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.45454545454545453Clontech Universal Primer Mix Long (96% over 25bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAAC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.45454545454545453Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453No Hit
GCGTTGATACCGCTGCTTCCCCTGTACTCTGCGTTGATACCACTGCTTCC10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.45454545454545453No Hit
ATACCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTGTGGTATCAA10.45454545454545453Clontech SMART CDS Primer II A (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCTGTCTCTTA10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAAAGCAAGCAAGCAGAATTTGGAATTCATCCAATCCAAATGCG10.45454545454545453No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGC10.45454545454545453No Hit
CCGATGCGGGGATCGAGCGCTCGCGATGCGTATCACACGGCTCCCGGCAC10.45454545454545453No Hit
TCCCCTGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTG10.45454545454545453Clontech Universal Primer Mix Long (96% over 27bp)
GATGCACAGCGGCCAAAGAGCCGCCGCGAGGAACACCGCCGGGGGCACAT10.45454545454545453No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGATGTCTCTTATACACA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTGATACCACTGCTTCCCAT10.45454545454545453No Hit
GTCATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCTTAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
AATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGC10.45454545454545453No Hit
TTTTGATACCACTGCTTCCCATGTACTCTGCGTTGGAAGCAGTGGTATCA10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGT10.45454545454545453Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAAC10.45454545454545453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGT10.45454545454545453No Hit
GGCTGGAGTGTAGTGGTGCGATCTCGGCTCACCGCAACCTCCACCTCCCG10.45454545454545453No Hit
TTTTTGCGTTGATACCACTGCCCATGTACTCTGCGTTGACTGTCTCTTAT10.45454545454545453No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.45454545454545453No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC10.45454545454545453No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAACAGAAT10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATGTCGGCGGCCTCGAACAACTGCTGGTCGGCCAGCAGCCACGAGTAGGT10.45454545454545453No Hit
ATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACGCAGAGTACATG10.45454545454545453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAAACAGAGG10.45454545454545453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAA10.45454545454545453No Hit
GTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACATCTCCG10.45454545454545453No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGCGTT10.45454545454545453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTTATACCACTG10.45454545454545453No Hit
GTACAGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGTATC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACTTTACCTTAGATAAAAATATGGAAGGTCCTGATCATGCTTTGTCAG10.45454545454545453No Hit

[FAIL]Adapter Content

Adapter graph