FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT219.545454545454547Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA115.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG83.6363636363636362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA62.727272727272727Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC62.727272727272727Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG52.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA41.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41.8181818181818181No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31.3636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31.3636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG31.3636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.3636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31.3636363636363635Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTCATCGTTACGCGTGTCAGCACCGATCAGGATGTTGGCGAGCAGATGG20.9090909090909091No Hit
AGACCACCCAGGGCTTCATCGGCTGGAGATCTGCAGTGCCAGGCCTGAAC20.9090909090909091No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.9090909090909091Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAAGGCAGAGTACATG20.9090909090909091Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAATGGGAAGCAGTGGTATCAACGCAGAGTAATGGGAAGCAGTGGTA20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.9090909090909091Clontech Universal Primer Mix Long (96% over 25bp)
GTGGAGTGCTGCTAGGGCTGCAATAATGAAGGGCAAGATGAAGTGAAAGG20.9090909090909091No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.9090909090909091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTACTCCTCCCACAAGGGACAGGCGTCCACCCGCCAGAAGAAGAGA20.9090909090909091No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.9090909090909091No Hit
GCTCCGCTTCCTTCTTCCTCATCTTCTTGCCTTTAAGTTGATCAACCAGG20.9090909090909091No Hit
GTATCAACGCAGAGTACATGGGATTCAAAAGAGGTCTTACAGCAACCCAA20.9090909090909091No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAAAAAATATT10.45454545454545453No Hit
CATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453Clontech Universal Primer Mix Long (95% over 24bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGAC10.45454545454545453No Hit
CCCATGTACTCAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCAATTAAAAATCCT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
TGGGCAAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCA10.45454545454545453Clontech Universal Primer Mix Long (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACACTTGC10.45454545454545453No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT10.45454545454545453No Hit
GGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10.45454545454545453No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
ATCTATATACTTGCACATTTAGTATTTGTCAATGTGCCAGAGGTTTTCTT10.45454545454545453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAACAAAGCAT10.45454545454545453No Hit
GTTCTTGTGTTGTGATAAGGGCGGAGAGGTTAAAGGAGCCACTTATTAGT10.45454545454545453No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.45454545454545453Clontech Universal Primer Mix Long (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
TCTTTGGGTCTGGCTGAAGTTGAGGATCTCTTACTCTCTAGGCCACGGAA10.45454545454545453No Hit
CAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCAC10.45454545454545453No Hit
TCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT10.45454545454545453No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACAGCTTCCCAATTACAA10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATAAACGC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTCGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGCCTGGGCCGCGGCGCCGGCAAATGGTTGGGCGTCACCGTGGGCATCGC10.45454545454545453No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG10.45454545454545453No Hit
TTGCCATCTTGTACCGGATTAATTCTCAGGCAGCCCAGCTTGAGACAGCG10.45454545454545453No Hit
GGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATCCG10.45454545454545453No Hit
GCTTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGAGAAGCAGTGGTATCAACGCAGAGTACATG10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGTCATGGTGAACTCTTGGCTAACAACAGCGCTAGCGGAGCGGTGCGAT10.45454545454545453No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGTTTT10.45454545454545453Clontech SMART CDS Primer II A (100% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GTACATGGGATGCAGTGGTATCAACGCAGAGTACATGGGATGCCGTGGTT10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGTAAGCGGTGGTAACAAGCAGAAGTACTT10.45454545454545453No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTAGTTTTTTTTTTTTTTTTT10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
ATCATTGTCCCTCCCCCTGTGCTCTCAGGCAATAGATGATTGGCTATTTC10.45454545454545453No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCCCGTCCAGGTAGGGGTGGGAGGTGGGCGAGCAGAAGGCCCTAGTTC10.45454545454545453No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAAAA10.45454545454545453No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACACA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.45454545454545453Clontech Universal Primer Mix Long (96% over 25bp)
AAGTAAGGAAGATGCTGGAGAATATGAGTGCCATGCATCCAATTCCCAAG10.45454545454545453No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC10.45454545454545453No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTG10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
TTATTGTAGCAAATGACTGAATAGTTCAGAGGTTACAAATTGGCTGCCCT10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGTTA10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG10.45454545454545453No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.45454545454545453Clontech SMART CDS Primer II A (96% over 26bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGCGGTA10.45454545454545453Clontech Universal Primer Mix Long (96% over 28bp)
GTCCTGATAGCCCGCAAGCGGGCTCCTACAAGTAACGCATCCAGCTGCTC10.45454545454545453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA10.45454545454545453Clontech Universal Primer Mix Long (96% over 27bp)
ACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTC10.45454545454545453No Hit
GCCAATGAAACCTGCACCAAGCAAAAGGCTCACGGCCAAAAAGTAGAGGG10.45454545454545453No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGTAAGCAGTG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
TTCCATGACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ATTGTATTCATCACCAGTCACCTCCACTTGGCCTTCATAGATCTTGTCCA10.45454545454545453No Hit
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGAA10.45454545454545453No Hit
GTTGGAGGTTGATGAATTTCATCAGTAGTGGGGGGCATGTCCCCTCTAGT10.45454545454545453No Hit
GGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTC10.45454545454545453No Hit
GATGTAGTGTTGACGCATCTTGCCGCTGATCGTGGCAAGAACGCGGTGCC10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGCTGTCTCTTAT10.45454545454545453Clontech SMART CDS Primer II A (100% over 26bp)
GAGTAATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453Clontech Universal Primer Mix Long (95% over 22bp)
GCGTGCCGCCGTACCAAGTGCGCTTCGCCGACGCGCGGCTGTACGAACAC10.45454545454545453No Hit
GAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTGATACCACTGCTTCCCAT10.45454545454545453No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453No Hit
GGTGAGGGGCATCAGGAGCACCACCGAGGCCCCGGACCCGGGTTACTACA10.45454545454545453No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCGGCTGCGA10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGACCTGTCACTTAT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTGCTCTGCGTTGAC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCATTGATACCACTGCTTCCCATGTACTCTACGTTGATACCACTG10.45454545454545453Clontech Universal Primer Mix Long (95% over 23bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.45454545454545453Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10.45454545454545453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTTTCACAGACCCCTGGCCTTGAGATTAGGAAGTGCCATTCCTGTCCC10.45454545454545453No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45454545454545453No Hit
GGATTTAGGGGCTGAGTTGAGGGGTATCTCCTTGAGCTCCGTCCGCATGC10.45454545454545453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCAATG10.45454545454545453No Hit
GTACCAGTTTGAGCGTGCGCTCGGCCAGCATCTGCCGAGTGCGGCTCAGG10.45454545454545453No Hit
GCGTCGTGGTACGTGTTGCAGCTGCTGAAGATGAAGCAGCACAAACAGCA10.45454545454545453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGAAGAGTACATGGGCTGTCTCTTAT10.45454545454545453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45454545454545453No Hit
GACCTGCCCCTCCGAGCCGTACCAGCGCAATGGCAGCGAAGTCCGTCAAT10.45454545454545453No Hit
GCTTCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.45454545454545453Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)