FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACACAGATGGCTGCAAGTTGGGAAGATGATAAAGTTACAGAAGCAT26.451612903225806No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT26.451612903225806No Hit
CGCCTACGCCGAGGCCATCACCGAGTGCCGGGTAACGAAGGTGCCCAAGA13.225806451612903No Hit
CGCGAGTGTGCGGCCGAAGCATGCGACGTGTACCTGATGTGGCCCGACAC13.225806451612903No Hit
GACCTCTCACGGAGCCCTCAGCCCTGGACAAGCTCAACTGCTCAGCTTCC13.225806451612903No Hit
GCAAGCTGAGAGGGAAGAGACGACATGGCGGCATTATCCCACGAGAGGGC13.225806451612903No Hit
GCGCCCTCCCGCGCGCGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGCG13.225806451612903No Hit
ACTTTGGAATTTAGGGCTAGAAGAGCCATCTGTTGTTAAAAGCTCTGTGG13.225806451612903No Hit
GGTGCATCCTGAGCTCTACGTGGACAAGTCGCGGGGAGATAAACTGAAGA13.225806451612903No Hit
ATCTTCCCACAACACCACAGGACTACAGGGTGCACAACTCCCCTGCCAAG13.225806451612903No Hit
CCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGAT13.225806451612903No Hit
GCACAAAAAGGGACAGTCGGAAGAAATTCAAAAGAAAAGAACCCGCCGAG13.225806451612903No Hit
GGCGAGTGTGCGGCCGAAGCATGCGACGTGTACCTGATGTGGCCCGACAC13.225806451612903No Hit
GCCCAACACGACCAGCGCGGTCGTGAGATAAGCCGCCGCCAACATGTGCA13.225806451612903No Hit
GCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCATCTGCTATA13.225806451612903No Hit
GCTTTAAATAGGAACCTGCTGTGAACTGGGGCAAAATTTGAAAATGAAGA13.225806451612903No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT13.225806451612903No Hit
GAGTACATGGGGCCCGCACCTGCTGGCTCACCTCCGAGCCACCTCTGCTG13.225806451612903No Hit
CCTCCAATCCCAGAGGCTGTCTCCCTGGTTCAGGGAGGGTGGCGGTCCAG13.225806451612903No Hit
GAACAGTGAATGGTGGGCCGCAGCATTGGCATGATGGGCCAGGGGTCCCA13.225806451612903No Hit
ACCAAATACCACCCATACCACAATAACTTATGGAAAAATGAAGTTAAAAA13.225806451612903No Hit
GGTCAGTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCAT13.225806451612903No Hit
TAGCTATTCATTCCAGATTTTCGTGTACCCATTCTGTTTCAGGAGCTCTT13.225806451612903No Hit
GTATTGGACCTTGTCTCCCGCCTGGACGTCCTGCTGACTCTTGTCAGTGC13.225806451612903No Hit
GATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCAC13.225806451612903No Hit
GTTCAGTAATTCTCGGCGCACGTTGTCGCGGCCGGCGAGAACATTGTGGA13.225806451612903No Hit
CGTTACTGGAATCCACAAATGAAACCTTTCATTTTTGGTGCTCGTAACGG13.225806451612903No Hit
TTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACCACCCTAGCA13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)