FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTCGCCATCCGCCGCGCCGCGCAGTTCCTGCTGGCGCTGGACGCCAT26.451612903225806No Hit
GAGTAGACACTGAACTTTCTGACTGACTGCCATTTGCTACTGATGTTTCA26.451612903225806No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG26.451612903225806No Hit
CACCACCTGTGCCCTAAGGTGGCTCTGCAACAGCCGCAGGGACAGGCTGA13.225806451612903No Hit
GTGTCGATATGGCGGAAACCTGCCTCCAGGGCGGCGCTGACGGCCTTCTC13.225806451612903No Hit
TTGGGATATAATTATCCAAGAAACTCTCCTTCACCTTAACAAGGGCGCAA13.225806451612903No Hit
GAACAGGCATATCTGCTAGCTCTCCTCTTGCAGTCCTCAGCCTCCCACAG13.225806451612903No Hit
GCCTCTGAGCTCACGGGGATACCATCTTTATCTAGTCGTTGCTTGAACAG13.225806451612903No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT13.225806451612903No Hit
TTGCAGTCTTTTTAGATGCTTGCTTAGCCTTTTTTGCTTCCTTAGCAGCC13.225806451612903No Hit
GGCCAGAAGCGCGAGCCCATCCACCGTGTTGGTTCCCTTTCCAAATTCAT13.225806451612903No Hit
ACTCATAGGAGTTCCAGGAGCCTCCATGAGAACTGTAAAGTCTTAACTTG13.225806451612903No Hit
ATTAGGGCTCGAGTGGCCTGCAAGGCCGCGGACAGGGCGAGCACCGAGTC13.225806451612903No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC13.225806451612903No Hit
ATACAGAGCCATTTCTCAGCACTCTTTTCCTTCCACTTAAGAAGCTGGAA13.225806451612903No Hit
ACTTGTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGG13.225806451612903No Hit
ATATAGCACTTTCCTTTTTAGCATGAAGGACTAATCACTGAAAATATAAC13.225806451612903No Hit
GAGCTGAGTCCACGAACTCGAACAGCAAGCAATCTCGCTGCTGCGAGCGA13.225806451612903No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGGGCCGCC13.225806451612903No Hit
CTACACCCAGGAGCAATTCTGGGCTGTGAAGGTACTGGGAGAAGGATCTC13.225806451612903No Hit
GTCCTGGTGGGTTAGGCGGAGGTCTATGGTCACGGCCCCGAGGCCATCGT13.225806451612903No Hit
CAACGGTGGTTTACGTAATATTGCTGACAATCTAATGCTGCTGCTTGAAC13.225806451612903No Hit
GCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAA13.225806451612903No Hit
GTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACT13.225806451612903No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT13.225806451612903No Hit
TTTTAATCATCACTAATATCTTAGTTCCAACTAACCAGCATCAATAATCC13.225806451612903No Hit
ACTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13.225806451612903Clontech Universal Primer Mix Long (95% over 23bp)
CGCTTCATGCACATGGTGGCGTCGGCTTATCTCACGACCGCGCTGGTCGT13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)