FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCACACCTGTTCCCCCATCTGAGAGATAATTACAACACTTACTGT44.545454545454546No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT33.4090909090909087Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGACACCACTGCTTCC33.4090909090909087Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTT22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
CTGATGGCCAGATCGTCGACATCGCGGAAGCGGCCGTGGAAGGTGATGCG22.272727272727273No Hit
GACAGCGACCCGATCCCGACCGCCAGCACCAGGCCCACCCAGTCCACCCG22.272727272727273No Hit
TGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGGAAG22.272727272727273Illumina Single End PCR Primer 1 (96% over 25bp)
ACTTAAAGGAAAAAAAGCGAAACCAACCTTCATGCAAAGATTAATTTTAA22.272727272727273No Hit
CAGCTCGATCTCCTGCTGCTGATAGCTCGGTGTCACCCCGGCGGTATCGG22.272727272727273No Hit
CCTTGCGGGTGTTGAAACTAAATGGCTGGCGGTCTTAGGCGAGGTCTTCG11.1363636363636365No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGCAATGTGACGTGACCACAGTATTTCTACTGAGGAGTCACCGATGT11.1363636363636365No Hit
GGCGCTATCTGCACCACAACTTCGTGGTGGCCCAGCTGAACGACTTGTTA11.1363636363636365No Hit
TTCAAGAACTTCACGTATCGCGTACGATCTCGAGCTTCAGCGACAATATT11.1363636363636365No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA11.1363636363636365No Hit
GGACATAAATGCGTAAGTATGATCAAGTATTTTGATGGGTGCAAGGTTTC11.1363636363636365No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
AAGACAAGGTCTGCAAAGGGTTTTGGGGCTCGAGGCCCAGACGTGCTGAG11.1363636363636365No Hit
GGCCGGCGCCAAGACCAAGGTCGGCAAGAAGGCCGGCAAGTCCGCCGCGG11.1363636363636365No Hit
CAAGATGAAACAACTCAAATTACATGGAAATACGTAAAACTATAAAAAAT11.1363636363636365No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATAAGCGTTGAAAC11.1363636363636365No Hit
GCGCTGCACGTCATGTGGGAGGACCTCGCCGAGGTCCGGACCCTGGTGGT11.1363636363636365No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTGATACAACAGCTG11.1363636363636365No Hit
TTGTTCGGCAGCTGAGCCTCCGTCCGGACCGGGCGGGTACACCACGGGCC11.1363636363636365No Hit
ACTCTGCGTTATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC11.1363636363636365Clontech SMART CDS Primer II A (95% over 24bp)
CCAGGGCGATTGTCTTTCCGGTCAACCTCTTTATGAAGACCTGCACCACA11.1363636363636365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTAAACATGAGATCGGCAGCTCCGTCGTGTAGGGAAGAGTTAGACTCG11.1363636363636365No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT11.1363636363636365Illumina Single End PCR Primer 1 (100% over 38bp)
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT11.1363636363636365No Hit
CGACACGGCAGGGGAACCTACCGCGGCGGGCGAGCGGATTCCTGTCTCTT11.1363636363636365No Hit
GTGTAGACTCGGTGGCTACCCATGTACTCTGCGTTGATACCACTGCTTCC11.1363636363636365Clontech SMART CDS Primer II A (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTATGCGTTTTTACCA11.1363636363636365No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTACCTGTCT11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTCC11.1363636363636365No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC11.1363636363636365No Hit
CCGTTGCCCACGGACGGGCCGCGTGCCACGCACGACGCGATCGTGGCCGT11.1363636363636365No Hit
TTACAAAAGAACTTCCAATGGAATATGCAGTTGAGCTTAACCGCTTGATT11.1363636363636365No Hit
TGATGGCTGCGAAGGTCTCGGTGGTCGAGGTCTTCGAGAAACCTTCCTCG11.1363636363636365No Hit
AGAGTGTAGATCTCGGTGGCCGTACACCCCTGTACTCTGCGTTGATACCA11.1363636363636365Illumina Single End PCR Primer 1 (95% over 22bp)
CTGCCAGATACAAAAGGGTCAGCCCCAGCGCCCAGCGCGCCTCTTTATGG11.1363636363636365No Hit
GGCCCTGGAAGACAGTGCCGAGGAAAAGGTGGAAGAAAGTAGGGCAGAGA11.1363636363636365No Hit
GGGAAAGAGTGTAGGTCTCGGTGGTCGCGTATCACCCATGTACTCTGCGT11.1363636363636365Illumina Single End PCR Primer 1 (96% over 28bp)
GGGTCAGGCTTCTTGCCCATGATCATCATGTCGTCCACCTCCACCAGGGG11.1363636363636365No Hit
GCGCTGGAGCGCGGTACCTTAAAAATCAAAAGCATTCAAATGACCTGGCT11.1363636363636365No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT11.1363636363636365No Hit
CAACATGCAAAGAATTGATATCAACGCACACTACAAAGGAACCTCACAAT11.1363636363636365No Hit
GATGTATGAACGCCAACACTCCCTATGAATCCAGCATTTTCATAAATTAT11.1363636363636365No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11.1363636363636365No Hit
GAGCACAGAACTGGTCCTCGGCGTTGTCGCCATCATCATATCTGTGGTTG11.1363636363636365No Hit
GAGCGTGCGCTCGCCCCCGATGAACCGGTGAGCGGAATCTCGTTCCACGA11.1363636363636365No Hit
CTGCAGACCAGACCTTCCCGAATTATCCGTGGCTCCGATGTCCCGGTGCT11.1363636363636365No Hit
CATTAAACAAGAACAAGAGTTAAAAACACAGACTAAAATAAACATCACCG11.1363636363636365No Hit
CGCTCTTCCGATCTCTGTACCGACAGAATCTGAGGCAAGCGCGTGTAGGA11.1363636363636365No Hit
CTTGGAGTCGACCTGGGCTTTGATCTTCGAGTTCTCGGTGGGACCGTCCT11.1363636363636365No Hit
CCCTTGTCGTATGTGTCCTGATCCATGCGTCGCTTTCCGTAGGTGATTTA11.1363636363636365No Hit
TTCTTGGGTGGGATCTTTCAACGGGGCTTGGAGAAGGAGGGACTGGCGGA11.1363636363636365No Hit
AACGTGAATTGAGGAGCCAAGAAACCCCAGAAAAGCCCCGGTCTTCAGTG11.1363636363636365No Hit
GGAACCAAAACAACATAAAAAAACACAATCACAAAAATACACAAATCCCA11.1363636363636365No Hit
GGTATCAACGCAGAGTACATGGGATGATCGGCGGCCCCGAGGTCTAAACA11.1363636363636365No Hit
TCTTCCTGCAGGTCTAAAATGTCGACCTTGACGCCCTTGGCGGCGAGAGC11.1363636363636365No Hit
GGCCTTGCCTGCGGGCTTACCCGCCCCACGCGCGGGCGACTTGCGGCGAT11.1363636363636365No Hit
CCCTTTGATTGAGTAGTATTGACAAAGACCTTGAGGAGATTTTTCTCTTG11.1363636363636365No Hit
ACGCTGAGTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAAGC11.1363636363636365Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTAGCTCCCGCGCAGCGTGGTGCCGGTCGGCAGCGTGTAGGCGGCGTT11.1363636363636365No Hit
GTAGATGCCCATCTTGGCGGTGACGAAGGCGTCGACGAAGTTCTGCCAGT11.1363636363636365No Hit
GCGCGTATCGAACCGTGGTGGTCGGCACGGACGGATCTGATTCGTCGTTG11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)