FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003641604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACTCACACACTTGAACTAAAGTGGATAGACAAGAT44.545454545454546No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTGTCAACGCAGAGTACATG33.4090909090909087Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATGGTAATGTAGCGACCACCGAGATTACACTCTTTCCCTACACGACGCTC33.4090909090909087Illumina Single End PCR Primer 1 (96% over 26bp)
CGGCTCGAAGGCGGCCAGTCCGCGCGCGGTGCTGACCGCCGCGATCGTCA22.272727272727273No Hit
ACGCAGAGTACATGGGAAGGCGACACCGTATTCTACCGACGCTCGTTTTT22.272727272727273No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCGATGTCGGAGTGGCGCCTGGCGGCGGCTTCCGGCAGTTCCCCGCC22.272727272727273No Hit
TATCAACGCAGAGTACATGGGCGACAGACCCTTCCTACACGACGCTCTTC22.272727272727273No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
TCCTAGAGGTGAGATATGGGAAACAGAAAGCAAATCAGTGTTTCCCATAT22.272727272727273No Hit
CTGTTGCTGGTGAACAACGACGACATCGTCGCGCCGGCTGCCGGCTTTGA22.272727272727273No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTGAAGGGGGAGCTTTTTCCTGAGGATATTTTCTGGTGATTTGACGT11.1363636363636365No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTGA11.1363636363636365Clontech SMART CDS Primer II A (95% over 21bp)
CCACCACCTTCTTCGGCATGTCCACCGTTACCACCGCGTCCGGATCGGTG11.1363636363636365No Hit
GCAGTGGTATCAACGCAGAGTACAGGGGTGTACGGCCACCGAGATCTACA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.1363636363636365Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATGGGTCTGGTGACCAACGCGGCCCGGCACGAACCGGAGGCCGTGGCC11.1363636363636365No Hit
GTACATGGGTAGCGCCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGA11.1363636363636365No Hit
GTGGAGATCCTCGGCAACATGGACGCCGAGTGCCTCTCCATCGTCTCGTT11.1363636363636365No Hit
CGGCAACACCACCTTCGCCGCCAACGCCGCCTACACGCTGCCGACCGGCA11.1363636363636365No Hit
TCTTCTGACGGTGCCGCGATCGAGATGCGTGACGGTGACGACGAGGACCT11.1363636363636365No Hit
TTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGT11.1363636363636365No Hit
GTACTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCACTGCTTCCCTGTCT11.1363636363636365No Hit
CCCTGGGGGGGGGGGGGCCCGTGGTTTTAAGGGCCCCCCCCCCCCCCGGG11.1363636363636365No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACT11.1363636363636365Illumina Single End PCR Primer 1 (100% over 24bp)
TCTTCAAGATACTTGATTGGGGGTAAATCTAAAAACAAATTTTACCCGAC11.1363636363636365No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG11.1363636363636365Illumina Single End PCR Primer 1 (100% over 22bp)
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG11.1363636363636365No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT11.1363636363636365Clontech Universal Primer Mix Long (96% over 25bp)
GGCTCTTTCACACCGTGTGCAAGGCTTTGTCCCCATTTGTAATTCCAGCA11.1363636363636365No Hit
ACCAAAAAGTCCTAACCCCTGCTCGGGGCCCCACGGATGCTGGTGGACTG11.1363636363636365No Hit
GACTACCTCTTTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGA11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGGATTGAAATTTGATCATTCTTCAGGAGACACCTTAATGAAACTCGA11.1363636363636365No Hit
GTGTTCTCTATGCCTTACTTGCTACAGTCTCCTCCTTCTTTGGAGAGGAT11.1363636363636365No Hit
CTCCTGGCCACCGCATGTACGCCGGCAGGTCCCGGCCAGGCCGCCGACGT11.1363636363636365No Hit
GCGATGGTCCCCATGAACAACGAGGAATGCTTCAGCGCCACCTAAATCCG11.1363636363636365No Hit
GCGTCACCCCAAGGAAACGCGCCGTGGCCTCGTGAGGCGGCGTACTCCCA11.1363636363636365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAGTACTT11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
GAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTG11.1363636363636365No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
ATGCCAGGAGAGCAACCACAGATATGATGATGGCGACAACGCCGAGGACC11.1363636363636365No Hit
TCTTCATACTCCAGCTGTCGCTTCAGCTTCAGCTTGTTGGAGAGGGCTAC11.1363636363636365No Hit
GTACATGGGAGTCGCCCCGAGATCTACCTCTTTCCTACACGCGCTTGCCT11.1363636363636365No Hit
GACAGAAGCGCAGCGCGACGATCGAAGCAGCGGCAACGCGAATGGTTAGA11.1363636363636365No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACC11.1363636363636365No Hit
ATCCAGAACCACCTGCTGCAGCTGCTGGCGTTGACCGCGATGGAAGAGCC11.1363636363636365No Hit
GCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGG11.1363636363636365Illumina Single End PCR Primer 1 (100% over 23bp)
ATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATGGGAAGCAGTG11.1363636363636365No Hit
AGCGAGCGTGCATCGCCCACCACCACCACGCGCAGGCCGCGCGTGTTCAC11.1363636363636365No Hit
ATTGACGGTAAGACCGAGGAGGTGCAGATTTTGAGGAAGGATCAAGAGAA11.1363636363636365No Hit
GGTGCAGACCCGCCACGGTCAGGACCGACTCCCCGGGCGGCAGATCCGCG11.1363636363636365No Hit
TCCTCGGCCAGCTCGACGAGGGCGTCGACCGGAGCGCCGACCACCGGCCG11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGACGCATCCAGCTCTCCGCGGACGCTGATCACTGCGGTAGCCGGCCACA11.1363636363636365No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
CCCTTCTAGTGATCATCAGCATTCTCATCATCGTCCCGGTGGCCTGGGTC11.1363636363636365No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
ATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTAACTCTGCGTAG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CAGCAGCTCGGCGTACACCTCCGGCTCGACGGGCGTCCCTTCGATGGGCA11.1363636363636365No Hit
GAATCCGCTCGCCCGCCGCGGTAGGTTCCCCTGCCGTGTCGCTGTCTCTT11.1363636363636365No Hit
CCACAGTCTTGCCACTGCACTCCAGCCTGGGTGATGGAGCGAGACCCTGT11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGCGAC11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAGAATGTGACAATCTGGTAAGGCATAGAAAAGATGCTCTCTCTGTA11.1363636363636365No Hit
GTTCCTGGAGAACGGATTCGAACGCTCGACCATCGACAGCATCGCCGCAT11.1363636363636365No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)