FastQCFastQC Report
Tue 10 May 2022
EGAF00003641917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35825
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1040.2903000697836706No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1010.2819260293091417No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA980.27355198883461274No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA920.2568039078855548No Hit
GTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA840.2344731332868109No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG830.23168178646196788No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC810.2260990928122819No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT790.22051639916259597No Hit
CCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAG780.21772505233775297No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA760.21214235868806697No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT710.19818562456385205No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT700.19539427773900908No Hit
CTATTGGCCAGGCTGATCTCGAACTCCCGACCTCAGGTGATCCACCTGCC620.1730635031402652No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC620.1730635031402652No Hit
GTATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGA620.1730635031402652No Hit
GTTTAATCCTGTGGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGG590.16468946266573622No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT580.16189811584089323No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT570.15910676901605023No Hit
GTATTAGCTCCATGACCCAGGCTGTTGACGTCTGCCCGGGCTGAAGTCAC560.15631542219120725No Hit
GTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGG550.15352407536636428No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG550.15352407536636428No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC540.15073272854152128No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG530.1479413817166783No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA530.1479413817166783No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC520.1451500348918353No Hit
TGATAAAGTAGCATGAATGTGAAGCTCTTTGCTTCCTGGCCCCTAAAACA520.1451500348918353No Hit
GAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAG500.13956734124214934No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT500.13956734124214934No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA490.13677599441730637No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG480.13398464759246337No Hit
CCCATATACTCCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCC460.1284019539427774No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG460.1284019539427774No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC450.1256106071179344No Hit
GTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTTGCTTTTTTTTTTT450.1256106071179344No Hit
GTACATGGGTCGCCCAGCCTGGAGTGCAGTGGTGCACACAATCTCAGCTC450.1256106071179344No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG450.1256106071179344No Hit
GTACATGGGGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATG430.12002791346824843No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC430.12002791346824843No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT420.11723656664340545No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC420.11723656664340545No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT400.11165387299371947No Hit
GGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGGCCCTTGCTACTC400.11165387299371947No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT400.11165387299371947No Hit
GGGTAATAGCATTCCTGAGATTGATGACATCCATTACCTCACTAGTCCAA400.11165387299371947No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGG400.11165387299371947No Hit
GTACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT390.10886252616887648No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC390.10886252616887648No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG390.10886252616887648No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT380.10607117934403348No Hit
GTACAGATGCAAAGTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGA380.10607117934403348No Hit
GTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGGGACCTGCTGCAGG380.10607117934403348No Hit
GTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGAGATTGATGACATC380.10607117934403348No Hit
GAGTATGGGGACGCCTGCACCAAGGCACACTCAGCACAGGAGCTGCAGGA380.10607117934403348No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA370.1032798325191905No Hit
AGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGA370.1032798325191905No Hit
ACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC360.10048848569434753No Hit
CATTAGTTCCTGGACATCTGTTGATCACATAATAAGACAACCTGCCCATA360.10048848569434753No Hit
ACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCT360.10048848569434753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)