FastQCFastQC Report
Tue 10 May 2022
EGAF00003641918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35825
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1230.34333565945568734No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1060.2958827634333566No Hit
CCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAG950.26517794836008374No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA920.2568039078855548No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA840.2344731332868109No Hit
CTATTGGCCAGGCTGATCTCGAACTCCCGACCTCAGGTGATCCACCTGCC800.22330774598743894No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG730.20376831821353802No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA710.19818562456385205No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT690.19260293091416608No Hit
GTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGG670.1870202372644801No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT660.18422889043963714No Hit
GGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGGCCCTTGCTACTC640.17864619678995114No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG640.17864619678995114No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC630.17585484996510817No Hit
GTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA620.1730635031402652No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT620.1730635031402652No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT610.1702721563154222No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC610.1702721563154222No Hit
TGATAAAGTAGCATGAATGTGAAGCTCTTTGCTTCCTGGCCCCTAAAACA600.1674808094905792No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC590.16468946266573622No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG590.16468946266573622No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT590.16468946266573622No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG580.16189811584089323No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG550.15352407536636428No Hit
GAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAG540.15073272854152128No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT530.1479413817166783No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG520.1451500348918353No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC500.13956734124214934No Hit
GTACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT500.13956734124214934No Hit
GTACAGATGCAAAGTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGA490.13677599441730637No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA490.13677599441730637No Hit
GTATTAGCTCCATGACCCAGGCTGTTGACGTCTGCCCGGGCTGAAGTCAC490.13677599441730637No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG480.13398464759246337No Hit
GTTTAATCCTGTGGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGG480.13398464759246337No Hit
GTATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGA460.1284019539427774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.1256106071179344No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG450.1256106071179344No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA450.1256106071179344No Hit
GTACATGGGGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATG440.12281926029309141No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT440.12281926029309141No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC430.12002791346824843No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT430.12002791346824843No Hit
GTACATGGGTCGCCCAGCCTGGAGTGCAGTGGTGCACACAATCTCAGCTC420.11723656664340545No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT410.11444521981856245No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG410.11444521981856245No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA410.11444521981856245No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA400.11165387299371947No Hit
GTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTTGCTTTTTTTTTTT400.11165387299371947No Hit
GTACATGGGTCGGGAGTTCGAGATCAGCCTGGCCAATAGGGTGAAACTCC400.11165387299371947No Hit
CTGTGAGTATGGGGACGCCTGCACCAAGGCACACTCAGCACAGGAGCTGC400.11165387299371947No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT390.10886252616887648No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA390.10886252616887648No Hit
GTGTATACACTGCTTGCATAAACTCAACCAGCTGCCTTTTTAAAGGGAGC390.10886252616887648No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA390.10886252616887648No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC380.10607117934403348No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA360.10048848569434753No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT360.10048848569434753No Hit
AGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGA360.10048848569434753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)