FastQCFastQC Report
Tue 10 May 2022
EGAF00003641966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003641966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21837
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT1130.5174703484910931No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC1020.4670971287264734No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA1000.4579383614965426No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT950.43504144342171547No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT810.3709300728121995No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA800.36635068919723407No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT790.3617713055822686No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC790.3617713055822686No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG720.32971562027751067No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA680.31139808581764894No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG640.2930805513577872No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT640.2930805513577872No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT620.2839217841278564No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG620.2839217841278564No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA610.27934240051289094No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA610.27934240051289094No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC600.27476301689792554No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG600.27476301689792554No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG560.2564454824380638No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA560.2564454824380638No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT560.2564454824380638No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA550.2518660988230984No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT530.24270733159316754No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG520.23812794797820214No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT510.2335485643632367No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC510.2335485643632367No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG510.2335485643632367No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC510.2335485643632367No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT500.2289691807482713No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC490.22438979713330587No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC470.215231029903375No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG460.21065164628840957No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA450.20607226267344414No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA450.20607226267344414No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG440.20149287905847874No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC430.1969134954435133No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA420.19233411182854787No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA420.19233411182854787No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG420.19233411182854787No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC410.18775472821358244No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC410.18775472821358244No Hit
CTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAG390.1785959609836516No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC380.17401657736868617No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT380.17401657736868617No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT370.16943719375372074No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA360.16485781013875533No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC350.1602784265237899No Hit
CTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAG340.15569904290882447No Hit
ATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAG340.15569904290882447No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC330.15111965929385904No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC330.15111965929385904No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT330.15111965929385904No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA320.1465402756788936No Hit
GGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACC320.1465402756788936No Hit
GTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTC320.1465402756788936No Hit
CTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGA310.1419608920639282No Hit
GTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACAC310.1419608920639282No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT310.1419608920639282No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG310.1419608920639282No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT310.1419608920639282No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA300.13738150844896277No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC290.13280212483399734No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC290.13280212483399734No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC290.13280212483399734No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG280.1282227412190319No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC280.1282227412190319No Hit
CCCCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGG280.1282227412190319No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC280.1282227412190319No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG280.1282227412190319No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT270.12364335760406649No Hit
CCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGC270.12364335760406649No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA270.12364335760406649No Hit
GTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGC260.11906397398910107No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG260.11906397398910107No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA260.11906397398910107No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT260.11906397398910107No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG260.11906397398910107No Hit
TTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTA260.11906397398910107No Hit
TAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGT250.11448459037413565No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG250.11448459037413565No Hit
CCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTG250.11448459037413565No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG250.11448459037413565No Hit
TTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGG250.11448459037413565No Hit
AGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCC250.11448459037413565No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC250.11448459037413565No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC240.1099052067591702No Hit
CCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCACCACCAC240.1099052067591702No Hit
ACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGC240.1099052067591702No Hit
CAATCAGGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGC240.1099052067591702No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA240.1099052067591702No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG240.1099052067591702No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC240.1099052067591702No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG240.1099052067591702No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG240.1099052067591702No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT230.10532582314420479No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGGTCAGTGG230.10532582314420479No Hit
GGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTT230.10532582314420479No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC230.10532582314420479No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA230.10532582314420479No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCAC220.10074643952923937No Hit
GCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTT220.10074643952923937No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC220.10074643952923937No Hit
GGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGC220.10074643952923937No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT220.10074643952923937No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA220.10074643952923937No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC220.10074643952923937No Hit
GTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGA220.10074643952923937No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)