FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC35.172413793103448No Hit
GATTACAAGTGCACATCAGCATGCCTGGCTAATTTTTGTTTTTTTTTTTT35.172413793103448No Hit
GGATAGGATGGTAGTTTTCAGTTTGATAGGTACATGGTAGTCTTGCACAG23.4482758620689653No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACA23.4482758620689653No Hit
GCTGAGGCGCTGCTGCTCAGGCGCTTCAACAGCATCACGCTGGCCGATCT23.4482758620689653No Hit
CAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGA23.4482758620689653No Hit
GAGAAGCGTATGGCCACAGAAGTTGCTGCTGACGCTCTGGGTGAAGAATG11.7241379310344827No Hit
TAACAACGGGAACCTTTGGCCTGAAGGTGCAAGAGGACACGGAGTTGCTG11.7241379310344827No Hit
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTT11.7241379310344827No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG11.7241379310344827No Hit
GCTCAGAACTCCGCGTCGAGCTCGTCGATCTCGTCGGGTTCCTGCTTCGC11.7241379310344827No Hit
CAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTG11.7241379310344827No Hit
GCGTACATCTCGGGCGGGGGGGGGGGGGGATTGGGAGCTGTGGGCAGAGG11.7241379310344827No Hit
GAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTG11.7241379310344827No Hit
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA11.7241379310344827No Hit
CTCTTCTCTGATCCTCAGGTAGTTGTTATCCCCACAGATTAAAACTGGCT11.7241379310344827No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTCCAGAACACAAACCTGACCCCATCACCTCTCTGCTTAGCCACTGG11.7241379310344827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAGCCTCCCCAGTTACTGGGACTACAGGCACATGCCACCATGCCTAG11.7241379310344827No Hit
GCACATGCGCGCGCCGAGCTTCATGAAGTCGAAGCCGAGCCTGTCGCCAG11.7241379310344827No Hit
TTGTGGCACTATTCACAATAGCAAAGACCTGGAACCAACTCAAATGTCCA11.7241379310344827No Hit
ACCCAACACAAATGGTTCCCAGTTTTTCATCTGCACTGCCAAGACTGAGT11.7241379310344827No Hit
GGGTGTATCTCATGTGGAGTGTTCTTACTACAAAATAATAATCATAATAA11.7241379310344827No Hit
AGCCTGGTCTCAGCATTCAGGGACTCAGAGAGAATAATCAGAGGTGCATT11.7241379310344827No Hit
CAGGAAATCTAAGTCTCCTATACCTGGGCAAGGCTACTTAGGAACGGAAC11.7241379310344827No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGGTCCTTGTTCTTGGCCACTGTCTGCAGCATGGGCAGAAAGTGCTC11.7241379310344827No Hit
ATCTGATGGTCTCAAGGGTCGTGTGTTTGAAGTGAGTCTTGCTGATTTGC11.7241379310344827No Hit
CCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG11.7241379310344827No Hit
GTGTAGCGGCCCGCGGTGTCGATGAGCACCGCCTCGTCGGTGAACCACCA11.7241379310344827No Hit
GACTGGAGACAGGAGGTCGGGAGAGGAAGAAGGGATGGCAGTGGTTGTTT11.7241379310344827No Hit
GGTTTCACCATGTTGGCCAGGGTGGTCTCCATCTCTTGACCTTGTGATCC11.7241379310344827No Hit
CCAGTGAACAGGCGCTCGCAAACGGGCAGACGTTGGCGAAAACGCTCGCG11.7241379310344827No Hit
GATCAAGATCATCGCACCCCCAGAGCGCAAGTACTCGGTGTGGATCGGTG11.7241379310344827No Hit
CAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCT11.7241379310344827No Hit
GCTTAAAGAGGACTTTGTAGGGGCAGTGACATCTGGTCTGGGCTGAATCT11.7241379310344827No Hit
GACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT11.7241379310344827No Hit
GACCATGATCCTCCAGGTGACGGGCATCTGGAACGACTTCCTGTTCGGCG11.7241379310344827No Hit
TTCTGCAGTGCTGCGCCAGATCGGGCCGCGGATCGCAGCCCGGGATTTCC11.7241379310344827No Hit
TCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAGAGGGTC11.7241379310344827No Hit
CCTACTACTGGGGCTTCGCCGCGTGGATGGCCTATTACATCAATCACCCT11.7241379310344827No Hit
GCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGG11.7241379310344827No Hit
AAGTTTACCTTTCTTTTGATAGAGCAGTTTTGAAACACTCTTTTTGTAGA11.7241379310344827No Hit
GTGCCAGGGAAAGCCACTCGTCAACACAATCTTCGGGGTGTGTTGGGGTG11.7241379310344827No Hit
GTCCATGATCCTCCAGGTGACGGGCATCTGGAACGACTTCCTGTTCGGCG11.7241379310344827No Hit
AAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGG11.7241379310344827No Hit
GGTGTTATCAACTTAAAAGATGCCACCTCTAAGGTAGCGCTGGTATATGG11.7241379310344827No Hit
CTCATGAGTAGGAAGAATCAATATTGTGAAAATGGCCATACTGCCCAAGG11.7241379310344827No Hit
TCTGCGGATAAAGCGCGTAGGACTGGAACACCATGCCGATGCCGCGCTCG11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)