FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTCCTCATTTTGCACACCTCACGGTTTACAAACACAGTCACATGATG35.172413793103448No Hit
ACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTG35.172413793103448No Hit
AAGAAGCCGTGCGACGCGGCGGCGAAGCGGTTGCGCGGCGCTCCGGTGTC23.4482758620689653No Hit
CCCTCTACGTTAGTGTAGTAGAAAGACAATTCTCCATGGTCAAGAGTTTA23.4482758620689653No Hit
TTCACGGCACCCGCGGCGATGCCGCGCACGAAGAGCTTGCCGGACGCGAA23.4482758620689653No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT23.4482758620689653No Hit
AGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCA23.4482758620689653No Hit
CAGCCAAATAGGAGAATCCAGAAAGGATGAAAGTAAAAAGATTAAAGCTG11.7241379310344827No Hit
GCAACGACGAAGGTGCGGCCGTCATCGAAAGCACGCTGCTCGGGCCCGAG11.7241379310344827No Hit
GGTATCAAGCAGAGTACATGGGACATGAGGGCTGTGTCAACTCTGTGTTA11.7241379310344827No Hit
CTCCCAGGCTGAACAGAGGGTCCCCCTAATTTGCTGTGGCTCTAGGAAGC11.7241379310344827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG11.7241379310344827No Hit
GTTTCCAACGGAGGCCCCAAAAACGCCTAATATTCACTTGCAGATTCTAC11.7241379310344827No Hit
GGGTGCTTCTTTGCATTATTGGCCTGAGCCTAAGGTCCTCACAGTGATAG11.7241379310344827No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT11.7241379310344827No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC11.7241379310344827No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT11.7241379310344827No Hit
CTCTCTTGGCAGGCTTTCTCTGACACCAATTCTACTCTAGCCCCTCACTT11.7241379310344827No Hit
TCTCCACGCTCAAGCAGATGCGGCTGCATGCCGCCGGCAGGAAGCCGGGC11.7241379310344827No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACG11.7241379310344827No Hit
CCTGTAATCCCAGCACTTTTGGAGGCTGAGGCAGGTGGAACACTTGAGCC11.7241379310344827No Hit
CAGGAAGAGCCACGTGAAGGGGTTCTTGGTGGGGTATGGGATCTTCCGCG11.7241379310344827No Hit
GAAGGCGCACAGCCCGCCGCGCTCGCCTCTCCGCCCCGCGTCCAGCTCGC11.7241379310344827No Hit
CCGCACCACTGCGAGGTCCTGGCCCCCGAGCCCCTGCTCCACCAGCGCTG11.7241379310344827No Hit
AGGTTGCGGTGGCTGAGCATGACGCCCTTCGAGCGCCCCGTGGTGCCACC11.7241379310344827No Hit
CGAAGCAAGTAACCATCGGTAGTCTTGACATCAACGTGAGCTTCAATCAT11.7241379310344827No Hit
CATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGC11.7241379310344827No Hit
GGGCACATATATTAGATTTAGGAAGACATTATGTTTGAATCCTGGCTTTA11.7241379310344827No Hit
TTCCCGTGTTGTGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC11.7241379310344827No Hit
GGAGAGGGGAGGGCAGGAGGAGTGAGAGGCCCAGACACTTCTTTTTTGCT11.7241379310344827No Hit
GTCTTCCCAGGCCCACCCTGGCGTCTGCCTATGGTAATCCCCTTCTGAAT11.7241379310344827No Hit
GGTGTGTGATGTTCCCCTTCTCATGTCCAAGGACCCTCCTGTTCAATTCC11.7241379310344827No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA11.7241379310344827No Hit
CCACAACACATGTGCTGTCTCTGTCTAGAGTGCATTTGCCAATCTCTGCC11.7241379310344827No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA11.7241379310344827No Hit
TTCGTGAGGCTAGCATGAGGTGTGTGCATTTGCCAGGGGCAAATTTCTAT11.7241379310344827No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAAGACACCCTGCTTCATGGGGAAACCTTGTTTGTCGTTCCCACCAC11.7241379310344827No Hit
GACACCTCTGAACCAGCCTGGGTGAAGCGAAAGATGTTATCAATAAATAG11.7241379310344827No Hit
TGCCGTGAAAGGCTGATCCCATGCTCGCCCCCAACGCGTCCGTCTCGGTC11.7241379310344827No Hit
GCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGG11.7241379310344827No Hit
GCCAAACACCACATGCTTGCCATCCAACCACTCAGTCTTGGCAGTGCAGA11.7241379310344827No Hit
GTTCTGTTCCATTGGTCTATATCTCTGTTTTGGTACCAGTACCATGCTGT11.7241379310344827No Hit
CGCGGCGCGCTGCCGATGAACATCAAGGCCCATTTCAAGAACTTCAAGGT11.7241379310344827No Hit
CGGTGGCTCACGCCTGTAATCCCACCACTTTGGGAGGCCGAGGCGGGCGG11.7241379310344827No Hit
CGGAGAGCTGAGCAGTCAAGATGTGTGACTTCACCGAAGACCAGACCGCA11.7241379310344827No Hit
TTCGGAGACGAAACGTGTCCACCCCATCGCTTCGATCGGATTGAGGTACC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)