FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003642047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCCAGCATCCCCAGATTGGCCGCCAGCCGTCCGGCGGACAGCCCTT28.695652173913043No Hit
GTTCACGACCGTGCCGTCGCCGTATCAGCGCGCCGACGCCGAGCGCTTCG28.695652173913043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28.695652173913043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCAGTGCTCAAAAAGT14.3478260869565215Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCGCGCTCAACGGTGCGGGCGCGGATGCGGTCGATGAGGGCGCTGTCTC14.3478260869565215No Hit
AACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGAT14.3478260869565215No Hit
AGCTACGGCTACATCCTGGAGAACGGCGAGATCTCGCTGCACGGGCCTGC14.3478260869565215No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
ATCCCCAAGCGCCGCTCACTCGCTTTTGTCTCGCCGTCGAGGAGCACCGG14.3478260869565215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTACCCATGTACTCTGCGTTGATACCACTGCTTCACATGTACTC14.3478260869565215Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG14.3478260869565215Illumina PCR Primer Index 1 (96% over 28bp)
CGCCTGCGCGGAGAGGGAGCGATTTGCCTGCAAACCAGTGCGAAAGAAAT14.3478260869565215No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14.3478260869565215No Hit
GTTCTGGTTCGTCCAGCCGCATGCCCACTCGCGTAGTGCGGACTGAACCG14.3478260869565215No Hit
CCCAGACTCTATGCCCGCCAGGCGCTGGAACGCTTTCGCGCGCTGCTGCC14.3478260869565215No Hit
GTACATGGGAATGATACGGCGACCACCGAGCTGTCTCTTATACACATCTC14.3478260869565215Illumina Single End PCR Primer 1 (95% over 23bp)
GGCGGTCACCGAGATGGATCGCGTTACCCAGCAGAACGCCTCGCTGGTGG14.3478260869565215No Hit
GGTCCGGGCGCCTTGCTTATGCTGCGAACCGATTTGCCCGACGGCGTCGA14.3478260869565215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACA14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)