FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003642048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCTGCTCATGTACAACGGCTCGTCGGCCTGCGTGTTTTCCTTGACGC28.695652173913043No Hit
GCCCACGTGTACTCCTCGCCTGCGTCCCACCACTCCGCCGCGAGCCGCAC28.695652173913043No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT28.695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGCAGGTACCAAGATCGAAGAGCGTCGAGGGAAGTGGGGAAAAAAA14.3478260869565215No Hit
TATTATGAAGATAAGTAAGTTCTTTTTCGTTCCCGCCTGACAACTGCCAG14.3478260869565215No Hit
TCGTCAGCCAGTTACGGGTTGACGGCGTTGCCGTGGATCGACGCCGTCGG14.3478260869565215No Hit
CGCCCTCATCGACCGCATCCGCGCCCGCACCGTTGAGCGCGACCTGTCTC14.3478260869565215No Hit
GCATGGAATTGCCTTATGGAGATCGTTGAACGTCAATGGTTGGCAGGTCT14.3478260869565215No Hit
CTCGGTGGTCGCCGTATCATTCCCATGTACCTGTCTCTTATACACATCTG14.3478260869565215Illumina Single End PCR Primer 1 (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14.3478260869565215No Hit
CGGTAACGCCGTGTTTCGTATGGACATGGCTGATATCGACGCCCATGCGG14.3478260869565215No Hit
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGCAAGATCTCGTAT14.3478260869565215TruSeq Adapter, Index 7 (96% over 33bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT14.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
GACTTAACCGGCTGGCCTGATCTTCCAGCGAGGCGGCTGCCGCAGCGGAC14.3478260869565215No Hit
TCTTCGTCTGGCTGTAGTTTCAGTGCGTTACACAGAGTTTGCCGACTGGC14.3478260869565215No Hit
CAATAAGCCACCGCATTGCAGTGAAGTGCCTGCTGAGGGACAACAGTGCC14.3478260869565215No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCATGGCTCTTATAAT14.3478260869565215No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG14.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
GTCAACGGGACCCACGCGATGGTGATCGCAGGAGACAATCCCTCCTCGAA14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)