FastQCFastQC Report
Tue 10 May 2022
EGAF00003642181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA270.40202501488981535No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT260.38713519952352593No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC210.3126861226920786No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA190.2829064919594997No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT190.2829064919594997No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT180.2680166765932102No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT180.2680166765932102No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA160.23823704586063135No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC150.22334723049434188No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC150.22334723049434188No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA150.22334723049434188No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT150.22334723049434188No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT140.2084574151280524No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC140.2084574151280524No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC140.2084574151280524No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT130.19356759976176297No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC130.19356759976176297No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA120.1786777843954735No Hit
TATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCATCAG120.1786777843954735No Hit
TTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGA120.1786777843954735No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG120.1786777843954735No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA110.16378796902918405No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT110.16378796902918405No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC110.16378796902918405No Hit
GTGCTAGATGGCGCCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCAT110.16378796902918405No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG100.14889815366289458No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG100.14889815366289458No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT100.14889815366289458No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC100.14889815366289458No Hit
GAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGT100.14889815366289458No Hit
CTCTATTTGTACTTGGGAGCAGGGCTGTCCCGACATCAGGCGCAGCAGCT90.1340083382966051No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG90.1340083382966051No Hit
GCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTT90.1340083382966051No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT80.11911852293031568No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT80.11911852293031568No Hit
CTATTTGTACTTGGGAGCAGGGCTGTCCCGACATCAGGCGCAGCAGCTGC80.11911852293031568No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA80.11911852293031568No Hit
CATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCC80.11911852293031568No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG80.11911852293031568No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT80.11911852293031568No Hit
CACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCA80.11911852293031568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11911852293031568No Hit
ATCATAAGGAGTGGTTTGGGAAACCTTTGAAAGATTCATTTTACTACAAA70.1042287075640262No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT70.1042287075640262No Hit
GTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCCTTGCC70.1042287075640262No Hit
GGTCTAAAGTGTTTAATTATCACTCACATATTTCACAGGAAAAGGAATGT70.1042287075640262No Hit
GTAGCAAAGGGGTCAAGATTGTAGCAAAAAACAAAAAATCCTGGATTTTA70.1042287075640262No Hit
CTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAAC70.1042287075640262No Hit
GTATCGTATTGAAAAAAAATCCAGGTTGTGCAGCTTGTTCTATTAACATC70.1042287075640262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)