FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGCAGTGATGAGAAGCAGCACACGGCAAGGCTGAGCCCTTTATCAG34.054054054054054No Hit
GGTCAGGTCACTTACAAAGGAAGCACCTCAGACTAATAGTGGACCTTTCA22.7027027027027026No Hit
GTCCATGAGCCGCTGGTACTCCTGATTCTGCCGCTCACTATCAGCTCGCA22.7027027027027026No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT22.7027027027027026No Hit
GCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAG22.7027027027027026No Hit
CATCTAACAACAAAATGGAACATGATGGTGCAAGTCACCATTTGGCTTAC22.7027027027027026No Hit
CTACAAGACCTGGTGCGGCGTTTGTGGCAGTGGTGGGCATGCTTGCGGGT22.7027027027027026No Hit
CAAGCATTCCTATACAGCAACAATAGACAAACTGAGAGCCAAATCATGAA11.3513513513513513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAC11.3513513513513513No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG11.3513513513513513No Hit
CAAGCCCGGCGTCGAGGACGTCGTCTTTTGCCATCTCCGCCTGTCTCTTA11.3513513513513513No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11.3513513513513513No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA11.3513513513513513No Hit
TTCCTTTACTTATCACCAGTGACTTGCATTTATTTACTTGAGTTTTTTAC11.3513513513513513No Hit
GCGGTCTCCTGGGGGAGTGCGCTACTCAGGCGCTGGAGAAATACAACATA11.3513513513513513No Hit
GTTCTAGGGAATCAAAGGAAAATAAGAATACTAGCCCCTGCTTTTTGGGC11.3513513513513513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.3513513513513513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGC11.3513513513513513No Hit
TATCAACGCAGAGTACATGGGAAGATGTCTGGAGGTTGGGAGCTGGAACT11.3513513513513513No Hit
GTCTTCACCTGGATCTCTCCAGTCTCGGGTGAGATGGTGAAGAGGCCCTT11.3513513513513513No Hit
GTGCTGACCGGCGCCGGCGGCCACTTCTGCGCGGGCGCCGACCTCTCCGC11.3513513513513513No Hit
AATAAGAACTGATACTACGTGGTATATGGAAACACTCGAAATGTAGATTA11.3513513513513513No Hit
ATTTAGGGCCCACCCTACTCTAGTATGACCTCATCTTAACAAATTACGTC11.3513513513513513No Hit
GCGGTACGGGTTGAACGAGCACGTGGAGCTCGACCCCTGTCTCTTATACA11.3513513513513513No Hit
CTAAGGCACTGGGCAGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGG11.3513513513513513No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC11.3513513513513513No Hit
GGTAGACATTGACTGGAATCTTTCCAAGCCCTTGGAAATGGAAATTTTTA11.3513513513513513No Hit
GTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAACCCCCTCGGCACCC11.3513513513513513No Hit
GGAATCCAACTTACAAGAGATGTGAAGGACCTCTTCAAGGAGAACTACAA11.3513513513513513No Hit
ATGGTCAGCTTCATCGCCGACGGGCTGCCCGACGACGCCCGCGCCGCGCT11.3513513513513513No Hit
AGCTCAGGGAGGACAGAAACCTCCCGAGGAGCAGAAGGGCAAAAGCTCGC11.3513513513513513No Hit
CTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTT11.3513513513513513No Hit
CGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCT11.3513513513513513No Hit
GTCGTCGACTCGCCGGACTCCTCCAGCAGGTGATTCGGCAGCCGATTGCC11.3513513513513513No Hit
GATTTGGAGAAGCGGATGAAGTTCTCCTTTAGTTCCGAAGTCAGCTCCTT11.3513513513513513No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT11.3513513513513513No Hit
CCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTT11.3513513513513513No Hit
TGCCTAAAAGATGCAAGAGTCCTTGGTTTGCCTCAGACTGGGGATGTGCG11.3513513513513513No Hit
TTTTAGTACTTTTCAGTTTTCAATGCTCCTTTAAGTCCTTTAGCCCATTT11.3513513513513513No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT11.3513513513513513No Hit
CCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTGTTGGGTTTTAAGCAG11.3513513513513513No Hit
CCCCTGTGGTCTCCTGGAATGTTGGATGGCTTTCTGGACAGACAGGGTCC11.3513513513513513No Hit
TCTTCCATCCCCATCCACCTCCGCTCTTTTTATTGTTTTGACACCCAGTG11.3513513513513513No Hit
GTGCTGCAGTAGTGGGGGAAAATGGAAGGTGGAGGGTGGAGTGTTTGCTG11.3513513513513513No Hit
AGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTG11.3513513513513513No Hit
GTTTGAGGCTGGTACCTGTTTCAGGCTAAGATGATACGTCGGTCATATGT11.3513513513513513No Hit
GAGTGGAGGGTTCCTTCCAGGCAGGAGCTGAGTCAGTACAGGCCTTGAGC11.3513513513513513No Hit
CATTGGTTTCGTTCTCAGCAAACTGACCTGGGCCACTCAACATCTGTCTC11.3513513513513513No Hit
GTGTATGTTGAACCAGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT11.3513513513513513No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC11.3513513513513513No Hit
GGCAGATTCTGGTGTATCAAGTTCTGGAAGGTCTGTCATAGGCTGTGGAA11.3513513513513513No Hit
GGGTGAAGCTGTAATTTTTCAAAAACAGTAAAAGCTGGTTTCTCCTAAAC11.3513513513513513No Hit
CCCCTACAACTTCTCGGCCGGTCCGGCTGCCATGCCGGAGGCGGTGCTGC11.3513513513513513No Hit
TCTGATACCAACCTGACTGGCTATTGGTAATTACTTCACAGCCAGAAGTT11.3513513513513513No Hit
GCTATGATGTGTTTGGAGCCCAGTCACCCTTTGGTGGCTACAAGATGTCG11.3513513513513513No Hit
GTTGGGGTGTCTCACCTAAAACAAGAACCACAGAAGGTTAAGGTGAGCTC11.3513513513513513No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA11.3513513513513513No Hit
CTCGAAGGAAGGCCTATGCCGATCGACGACTCGCGTCCGATCTGGATCCA11.3513513513513513No Hit
GTCGGGATCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCTAGG11.3513513513513513No Hit
GTGTGTCCTTATTCATCACCAGCAGCTTTGAAAATGAAGTGCTTAAGAGG11.3513513513513513No Hit
CCTGTTGGCCATACAGGGCCCCAATGCCACCAAAATATTACAACCATTAG11.3513513513513513No Hit
CGTGGGCGTCGATGCGCTGCGGCAGCGTCTCCACGCCCTGGAGCAGGTTC11.3513513513513513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGATTAT11.3513513513513513Clontech Universal Primer Mix Long (96% over 26bp)
TCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGC11.3513513513513513No Hit
GGTCAGCACTGCTCTCAACAGACAGCTGGGGTAGGAGGTAGGCTGCTGGT11.3513513513513513No Hit
TCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAG11.3513513513513513No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)