FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG70.274079874706343No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG70.274079874706343No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA60.23492560689115116No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG60.23492560689115116No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT50.19577133907595928No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT50.19577133907595928No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG50.19577133907595928No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG50.19577133907595928No Hit
GGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACA50.19577133907595928No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT50.19577133907595928No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA50.19577133907595928No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG50.19577133907595928No Hit
GTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTC50.19577133907595928No Hit
GCCTGATGGAGGAGAGCTGGTCCTGGGGGGCTCGGACCCGGCACACTACA50.19577133907595928No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG50.19577133907595928No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT40.15661707126076743No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA40.15661707126076743No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC40.15661707126076743No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA40.15661707126076743No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC40.15661707126076743No Hit
CCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAG40.15661707126076743No Hit
AGGCTGGAGTGCAGTGGTGCAATCTCGGCTCACTGCAACCTCTGCCTCCT40.15661707126076743No Hit
CTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGG40.15661707126076743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA40.15661707126076743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA40.15661707126076743No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC40.15661707126076743No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG40.15661707126076743No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG40.15661707126076743No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG40.15661707126076743No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG40.15661707126076743No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC40.15661707126076743No Hit
ATTATAAGGTGGATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGC40.15661707126076743No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC40.15661707126076743No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA40.15661707126076743No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG40.15661707126076743No Hit
ATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGG40.15661707126076743No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCA30.11746280344557558No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG30.11746280344557558No Hit
GTGAAGGACGTTTGTGTTGGAAGCCCTGGTATCCCCGGCACTCCTGGATC30.11746280344557558No Hit
ACTCAATTGGGCAATTAACATTAGTGTTTGTTCTCTGATGGTATCTCTGA30.11746280344557558No Hit
ATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTC30.11746280344557558No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA30.11746280344557558No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC30.11746280344557558No Hit
GAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGC30.11746280344557558No Hit
TTTATGAAATATATCTTTGTATTTGCCTTAATTGCCAGGGATATGGGAGG30.11746280344557558No Hit
GTACATGGGGCCCTTCTGGATCCTGCATTGCACCCTGGTGTACCCACTGG30.11746280344557558No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA30.11746280344557558No Hit
ACCTCATCCTTGGCCTGTGCCCAGGGCCCTGAGTTCTGGTGCCAAAGCCT30.11746280344557558No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT30.11746280344557558No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGG30.11746280344557558No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC30.11746280344557558No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT30.11746280344557558No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG30.11746280344557558No Hit
TATCTTAACATGTCAAAGGAGCTCAGGTCTGAGCGCAGCTGCTTTGCTTT30.11746280344557558No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT30.11746280344557558No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG30.11746280344557558No Hit
CAAGATGGCCACCGCGCCCGCTAGTCATCTCGCTAGCCCGCGCGCCAGTG30.11746280344557558No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGCTGTC30.11746280344557558No Hit
ATACAATCTGGATCGACTCCACAGGAAGCTTTCGCTGTAGCTTGACGTTG30.11746280344557558No Hit
CCGTAGCGGCGCGCGAGCGGGCCGGGCGGGCGGCCGAGTTTTCCAAGAGA30.11746280344557558No Hit
GGTTGGACCTCTTAGCCTGCTTATGAAGCCTGAATTGACATCATGCCAAC30.11746280344557558No Hit
CACCTGGGGGATGTCCTGAATTTTTTACCCCTCTAGCCATGGCCACTGAG30.11746280344557558No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG30.11746280344557558No Hit
GAGGGACTCTGCAACAAGGACTTCTTCCAGAAGATGAAGGAAACAGCTGT30.11746280344557558No Hit
ATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCT30.11746280344557558No Hit
GGACAGGGAGGATCCTGTCTGGCCTTCAGTTTCCATCCCCAGGATCCACT30.11746280344557558No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG30.11746280344557558No Hit
CTTCTACCCCAAGGCCTCACTGCTGCCATCTGTAAGTGAAGGAACTGGAC30.11746280344557558No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC30.11746280344557558No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG30.11746280344557558No Hit
CCTTCTACCCCAAGACCTCACTGCTGCCATCTGTAAGTGAAGGAACTGGA30.11746280344557558No Hit
GAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCC30.11746280344557558No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA30.11746280344557558No Hit
TTCCATTCTTCACCCAGAGCGTCAGCAGCAACTTCTGTGGCCATACGCTT30.11746280344557558No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC30.11746280344557558No Hit
TAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCC30.11746280344557558No Hit
AGTCAGGGCCCATCAGGGGAATGAAGTGGCTAAGGGTGCCTCCAGCTCTA30.11746280344557558No Hit
CCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG30.11746280344557558No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC30.11746280344557558No Hit
GCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCG30.11746280344557558No Hit
GGCGTGACGACCCTGGACATCGTCAGAGCCAACACCTTTGTTGCAGAGCT30.11746280344557558No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGG30.11746280344557558No Hit
TGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCA30.11746280344557558No Hit
GTGAGGCCACAGCTACTGCAGCGCTTTTTATTTATTTATTTATTTACTGA30.11746280344557558No Hit
CTCCCGCTCAGAGGCAAAGACCGGGGGCGTCAATCTCTGCCCCCAACAGC30.11746280344557558No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT30.11746280344557558No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC30.11746280344557558No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC30.11746280344557558No Hit
GGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA30.11746280344557558No Hit
CCTGTGCACAGCAGCCAGATGGCCTGGTTCACACGGCGCAGGGGGGACAC30.11746280344557558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)