FastQCFastQC Report
Tue 10 May 2022
EGAF00003642458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18657
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGGCGGGTGATAAGAAACGCAAGAGTCCTGCAGCCAAGAA520.27871576352039446No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG490.26263600793267944No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT410.2197566596987726No Hit
GTACATGGGACGATCGTCGCCGTCCTCTGCTTCGTCGCCGTGACGGCCAG380.2036769041110575No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG360.1929570670525808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1929570670525808No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT330.17687731146486574No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA320.17151739293562737No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA310.16615747440638903No Hit
GTACATGGGAGCGCGGCTCAGCCATGAGCAACAGGAGTAGCTTTTCCCGG310.16615747440638903No Hit
GTATCAACGCAGAGTACATGGGACGATCGTCGCCGTCCTCTGCTTCGTCG290.15543763734791233No Hit
GTATCAACGCAGAGTACATGGGAAGGCGGGTGATAAGAAACGCAAGAGTC280.15007771881867396No Hit
CCATATAGCTACGGGAACCTAGTCCATATCGCTTTCACTCTCGCTCTCAC280.15007771881867396No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA270.1447178002894356No Hit
ATCTTTAACAGTCCTCCTCTCTTTTTTGAATCAAGGGTTCCAGTTAAGCT250.1339979632309589No Hit
TATCAACGCAGAGTACATGGGACGATCGTCGCCGTCCTCTGCTTCGTCGC240.12863804470172052No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT220.11791820764324383No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG220.11791820764324383No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT210.11255828911400548No Hit
GTACAAGTATGTCGTTCGTCCTAAGAAGAAGAGCGCCTCTTCCGCTGAGA210.11255828911400548No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC200.10719837058476712No Hit
GTACATGGGACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATG200.10719837058476712No Hit
GTACATGGGAGTGCGTCCTACAGATACCACAACCTGGATCAGGTCCAAGC200.10719837058476712No Hit
ACATTACACATAACATTAAAACTGAAGGGGAAAAAAAAACCAAAAACCAG200.10719837058476712No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG190.10183845205552874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10183845205552874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)