FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42.73972602739726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGGTTTATGGGGTTGTGATGTGTTTGTGCCAAAATGTCTCAGTG42.73972602739726No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTACGACCAGATC32.054794520547945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.36986301369863Clontech SMART CDS Primer II A (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGT21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TCTCTCGGGGGCATCCCGAACCTGTCGCTGATCGGCCAGGTGGACCTCAT21.36986301369863No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGAGACGGTGCGCCGTTCACCCATGTACTCTGCGTTGATACCACTGC21.36986301369863Clontech SMART CDS Primer II A (95% over 24bp)
CTGGGACACAACAAAGATGCCCACTGTCACCACTGTTATTCAGCATAGTA21.36986301369863No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGAAAAAAAA21.36986301369863No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAACAAGATGTCAGGGCCGGTTCAGGGAGTTCATCTCCAGCCACCTG21.36986301369863No Hit
CACATCCTATAAAAGTCCTTGTCATCTCTGTGATGGGACATGTGTTGATG21.36986301369863No Hit
CCCATTTCTCCAGCACCCCAGAATTAAATCTGCTCTGAGGAAAATAAAGG21.36986301369863No Hit
GTATTTGCCGGGCGCGCCGCTGTGGGTTCTCGATGAACCCTTCACTGCCC21.36986301369863No Hit
ACTTGGGACAGAGTGTAATGTGGGCCATCCCATGTACCCGTCTCTTATAC21.36986301369863No Hit
ATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAG21.36986301369863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGTGTACGGGCACGAGCTACACTCTTTCCCTACACGACGCTCTTC10.684931506849315Illumina Single End PCR Primer 1 (96% over 32bp)
GGTATCAACGCAGAGTACATGGGAGGCAGTGGTATCAACGCAGAGTACAT10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGG10.684931506849315No Hit
TCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGG10.684931506849315No Hit
GATTAAATAGAAGGTTTGTGCCAGTAGACATTATGTTACTAAATCAGCAC10.684931506849315No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACA10.684931506849315No Hit
AAAGAGTGTAGATCTCGGTGGTCGCGATCATCCCATGTACTCTGCGTTGA10.684931506849315Illumina Single End PCR Primer 1 (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AATCATGCAACGACTTAACTATATCCCTAAAAGAAAAAAATCAGAAAACA10.684931506849315No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAA10.684931506849315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.684931506849315No Hit
CACATCAGCATCGGCCGGGTCGGCACCCAGTTGCGCAGGTCGTTGGCGAC10.684931506849315No Hit
ATTTCCAAGGGTCTAGCCTTCACTCAAACTTTCCACTTTTAGAATCAAGG10.684931506849315No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACC10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
ACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGG10.684931506849315No Hit
GCATCACAGAGAGCGGAAAAGAGCCAGCAAATCCAGCAAGCACAGCATGA10.684931506849315No Hit
CCGTACTTCCCATGTACTCTGCGTTGATACCACTCTGTCTCTTATACACA10.684931506849315Clontech SMART CDS Primer II A (95% over 22bp)
CGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATC10.684931506849315No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCGTTTAAACCAAAC10.684931506849315No Hit
TATCAACGCAGAGTACATGGGGCAGAAGTCGGCAATCGAATTATTTTTTT10.684931506849315No Hit
GTGGTATCAACGCAGAGTTCATGGGAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGATAACACTGGGTA10.684931506849315No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATTACTCCTGTCTCTTATA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAATAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
ATGGTGCACGTGGACTTTTAGCAAGCGGGCTCACTGGAAGAGACTGAACC10.684931506849315No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
GGCGTGAGCCACCGCGCCTGGCCGAGACCAGATCCCTTTCTGTCTTTGCT10.684931506849315No Hit
GATAGTATCAAAACTATTTATAGTATCAAAAATAATAAAATGCTAAGGAA10.684931506849315No Hit
GTGTAGGGAAAGAGTGATTCGGTGGCCCCATGTACTCTGCGTTGATCTGT10.684931506849315No Hit
GAGTGAGACTCCATCTCAAAAAACAAAAAACAACAAAAGAAAGAAAGTTG10.684931506849315No Hit
GTATTAGATTCCTCTTCTGCGTTTGCCTAGAATGGTTTATATCATTTAAC10.684931506849315No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CACCTCGACGTCGCCGGCGGCACTTTGGGCTTTTTCGATTGCGCATTCAG10.684931506849315No Hit
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAATCTCGGGTCGCCGTACT10.684931506849315Illumina Single End PCR Primer 1 (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTTTCCACCACTATCGGCTGTTCTGGAAGACTGGGAGGAGGGGTCTTGC10.684931506849315No Hit
GCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCTG10.684931506849315No Hit
TTCTAGGGCTGGCATTGAGTGCCTGTGGCTTTTCCAGGCACATCTGTCTC10.684931506849315No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGATACCAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCAGAACACCCTTGGGGCGCAACTTCCCGGTGAACGTCCCGTCTCCTCAG10.684931506849315No Hit
GATAAACATATAATTTATCATTATTCATCATCATAAAACTTAAAATTATT10.684931506849315No Hit
ATTATTGTGTGTATGTATGTGTGTGTGTGTGTGTGTGTGTGTGAAGAATG10.684931506849315No Hit
CATGCAGCCCCTGGCAGAGTAGTTAGCAATGAAAGGTGCATATTAAAAGT10.684931506849315No Hit
GATGACGCGCAGATCGCCCTCGGCCAGGCGCTGGTCGAGCTGCGAGACCT10.684931506849315No Hit
GTATCAACGCATAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGATTACACTCTTTCCCTGCTGGTACCAGTAGACAGTGAGATCGGAAGAG10.684931506849315No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.684931506849315Clontech SMART CDS Primer II A (100% over 26bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
TGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAGTACT10.684931506849315No Hit
TGACAATGAAATTAGTGCCTGTTTTCTTGCTTCTTCCCCACCCTGTCTCT10.684931506849315No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACA10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAATCATTAAATGGACTCTAGTGGAATCACCATCGATTGGAATCGAATGG10.684931506849315No Hit
ACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAATAAAAAAAAAAAAA10.684931506849315No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
CAATTGCCGCATTGTCAAAGCAGTTGCCCTTTCGGCTCATGCTTTGCTTG10.684931506849315No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACGACGAAGACTCCGCCTTGCTCGAGCCTGGGAAAGTACCGAATGA10.684931506849315No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAATATTCCCTTTCACAGAGTAGGTTTGAAACACTCTTTTTGTAGTA10.684931506849315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGAT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCACAGCCTG10.684931506849315No Hit
CTCTTACTGTGTGCTCAATGGCAACTTTCCATTTCTTTATTTCTTGTAGT10.684931506849315No Hit
CTCTTACTGTGTGCTCAATGGCAACTTTCCATTTCTTTATTTATTGTAGT10.684931506849315No Hit
TCTCGGTGGTCGCCGTATCACCCATGTACTCTGCGTTGATACCACTGCTT10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTTAGAAAGACGTTGGACCATTTCTTAGAAAGTTCAACCCAAACTTAACA10.684931506849315No Hit
GAGTACATGGGGGCTCGGCCGGCTGGTACCTCCCGCCCAAGGGCTACCTC10.684931506849315No Hit
CTCAAGACTCAACTCTCCTTCCGGTTCGCGATTCTTGATCAAGTACTTTT10.684931506849315No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACTGTCTCTTA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
TTCCCTACACGACGCTCTTCCGATCTCAGTCCCCGCATCATGGTCTGAGA10.684931506849315Illumina Single End PCR Primer 1 (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGATGCGGCGCCAGAGATACACTCTTT10.684931506849315No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTGGCGTCTGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCACAAG10.684931506849315No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATTAGGCGGTGTTAAA10.684931506849315No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGGCATTCTCGTCTCAACACCTCGTTACAGATGGGGAAGTGGATCCA10.684931506849315No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATCTCTTTCAGATCTCGTA10.684931506849315No Hit
AGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACC10.684931506849315No Hit
CTACTGAGAACCGCGAAAAAAAACATTTCAATCGGTAAAACCGCTCTATA10.684931506849315No Hit
ACGCAGAGTACATGGGAGACCAGATCACCCTTCCCTACACGACGCTCTTC10.684931506849315Illumina Single End Sequencing Primer (95% over 22bp)
CAATAAACAAAGACTACATTTGAAGAAGAAATATAAAAACACATTACAAT10.684931506849315No Hit
GCGTCTGAGTCTGAAGGCCAAAGTCCTGTCCCTCGCAGTTGTGCCGGTGT10.684931506849315No Hit
GGATATGAAACACAAAAGGATAAATTGGGGTGAGGTTAAATTTGAGAGAG10.684931506849315No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAATTTCGTTTATAACAC10.684931506849315No Hit
CTGTACTACAACGAAGACGGAATTATTCCACAACTGCAAGCCGTGGCGGA10.684931506849315No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGATAGCCCACGAGATCTA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAAATACAGTATGATCTTAGGAGACCGTTGTACTTCGGTGACCAAAA10.684931506849315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)