FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64.10958904109589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53.4246575342465753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGTAATTGACTTCTAACAAGGTAGATAATTTTGATCATGTTAGAGAC42.73972602739726No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32.054794520547945Clontech Universal Primer Mix Long (95% over 21bp)
AATCTGAGGCAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTGGTCGTAC32.054794520547945Illumina Single End PCR Primer 1 (97% over 34bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32.054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
TTACTATGCTGAATAACAGTGGTGACAGTGGGCATCTTTGTTGTGTCCCA21.36986301369863No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTTGGGGGATGATTAGGTTATGAGAACAGCACACAGTTCGTGTTTTC21.36986301369863No Hit
TTGTACATGAAAGCATTTATCAACTGCAAGTCATGATACTAATGTGAGGA21.36986301369863No Hit
ACCCTGTTGTCAATGCCTCTGGGTTCATAGAAGGTTCAAGGGCCAGGTCT21.36986301369863No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA21.36986301369863Clontech Universal Primer Mix Long (95% over 21bp)
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG21.36986301369863No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.36986301369863Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCGGGAACAACGCAATCACGATGGCAAAGAACACCAGCGGGTTGGCCAA21.36986301369863No Hit
ACCTCGTACAGCGGGGTGAAGAACATCGCCAGCAGGAACAACCCGCCCGT21.36986301369863No Hit
ATAAAATAACTAGAATGGAAACAGAAAATTAGATGTTCCATATGTTGTAA21.36986301369863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
ATGATGAACAGAGAAAAAGTGCAGTGGGACACGCTGGCAAGACTCTTACT21.36986301369863No Hit
CCATTTGATTATTCCATTCAACTCCATTTGATGTTTTCTTTCAATTCCAC10.684931506849315No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGGCCACCGAATCACTCTTTCCCTACACCTGT10.684931506849315No Hit
CTTTGAATCACTTCCAAATCTACTGATTTTGAGGTTCCGCAGTAGTTCTA10.684931506849315No Hit
GTACATGGGAAGCAGTGGTATCACTGCTTCCCATGTACTCTGCGTTGATA10.684931506849315No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.684931506849315No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTATCGTGGTAGCAGGCTGCGCCTGCTGGAGATCGGAATCCAGAATG10.684931506849315No Hit
GTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGACG10.684931506849315No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CAGAAAACTGGTACTGTCCTGGCCAGGGCTCATGAAAGTTCCAGGGCACA10.684931506849315No Hit
TCCCTACACGACGCTCTTCCGAATGAGGAACTGGAGGCCCAGATCGGAAG10.684931506849315Illumina Single End PCR Primer 1 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.684931506849315No Hit
GAGTACATGGGATAGCGACCCAGACTACACCTTTCCCTACACGACGCTCT10.684931506849315Illumina Single End Sequencing Primer (95% over 21bp)
TCCCTACACGACGCTCTTCCGATCTCATGATGCAGCAACTCCAGTCCTTG10.684931506849315Illumina Single End PCR Primer 1 (100% over 25bp)
GAGTAATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTTCAAGCAAGTGAAAAGGAAGAAGTTGTGGATTGATTTGGGAAGTA10.684931506849315No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACC10.684931506849315Illumina Single End PCR Primer 1 (100% over 25bp)
CAGTGGTATCAAAGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.684931506849315Clontech Universal Primer Mix Long (100% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGC10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
CGTATACATGTACTCTGCGTTGAAACCACTGCTTCCCATGTACTCTGCGT10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGCAGACGTTAAGCGAGTTCCGTGTTTATGCGCTGGGCCTTGGCAGACA10.684931506849315No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATTAATA10.684931506849315Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTGAAAATGCTGGGATTACAGGCATGAGCCATTGCGCCCGGCTATCCACT10.684931506849315No Hit
CCCTTTACGAGCACCGTCTCTAGAGGTTTAATTGCTTTGTCCTTTCTAAA10.684931506849315No Hit
GAGGGTGCAGCTGTGGCAGCATCTGTGGGTGGCTGAGGAGACGGGACGTT10.684931506849315No Hit
GTTCTAACTCATGCTGTGCTTGCTGGATTTAGAACTCAGACTCCCATAAC10.684931506849315No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATAAATAATTTAT10.684931506849315No Hit
CCGTTGGTGTCCGCCGAGGTCTGATCCGCCGCTGGCGTGTCACCGCCGTC10.684931506849315No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAATAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCCTGT10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA10.684931506849315No Hit
GAACTTCGCCAAGTGCGTGACCAACGGCGTCATCCCGCTGGGCGGCGTGA10.684931506849315No Hit
GGATTGACCGGTACGCGTCACGGACTGGTTTTATTTCTTGTTTTTAAAAT10.684931506849315No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGC10.684931506849315No Hit
GTACATGGGAGATACGCGACCACCGAGATTACACTCTTTCCCTACACGAC10.684931506849315Illumina Single End PCR Primer 1 (95% over 22bp)
ACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.684931506849315No Hit
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGACCA10.684931506849315Clontech Universal Primer Mix Long (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCGTGTAGGAAGATGTAGATCTCGTGGGCTATCCCATGTACTCTGCG10.684931506849315No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.684931506849315Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTGGTAT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAGTTTTTT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CATTATTACCTCCCAAATTCTACCTTCATTTCCCTGCACAGTTTCACTGG10.684931506849315No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGAACGGCGACCACCGAGATTACACTCTTTCCCTACACGAC10.684931506849315Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGGAACTGATCGTCCACTCGGACCAAGGTTGGCACTACAAGATGCAGCC10.684931506849315No Hit
GTAAATTGGATGGCCCACATTACAAACAGTCCCAAGTCAGTCTATTATAA10.684931506849315No Hit
GACCTTCCATACCGAAAGTACGCGCTTCGTCGGCGATGATCGGTACCAGA10.684931506849315No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCCAGGCTGGAGTGCGGTGGTGCCATCTCGGCTCACTGCAACTTCTGCCT10.684931506849315No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCC10.684931506849315Clontech Universal Primer Mix Long (96% over 27bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCTG10.684931506849315No Hit
GTCCTGGAGACTGGGAAATCTGAGAGCAAGATGCCAGCAGATTCAATGCC10.684931506849315No Hit
ACGCAGAGTACATGGGGTAAGCAGTGGTATCAACGCAGTGTACATGGGAA10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCTAAAGTCACTATTATCAATCAAACCACAAAGGAGGTTAATGCAACTTC10.684931506849315No Hit
ATGTGCCTGGAAAAGCCACAGGCACTCAATGCCAGCCCTAGAACTGTCTC10.684931506849315No Hit
TGTATATAAATGGCCACTCTTTCCTTCCTAAGACTGAACATTTGCATTCC10.684931506849315No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT10.684931506849315Clontech SMART CDS Primer II A (100% over 26bp)
GCGCCGTTATGGCTGCAACTGTCGGCCGCGTTCTCGCCGCAACGTCTGGC10.684931506849315No Hit
ACAGAAAGACTGTTTCAAAACTGCTCTGTCAATAGAAAGGTTCAACTCTG10.684931506849315No Hit
GTACATGGGATGGCCCACAATAAACTCTGTCCCAAGTCTGTCTCTTATAC10.684931506849315No Hit
TCATCCAGTGTTACTAGATGTAATACTGTATGATTTGGGAAAACATTGAT10.684931506849315No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCACAGCAT10.684931506849315No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGCCAGCGGCATCGACACGCTGCTGCCGAGCGCATCGCCCGGCACGC10.684931506849315No Hit
ATCTAATTTGCCTATCTGTGAATATTTATAGACTTTATTTCCACAGCAAT10.684931506849315No Hit
GTACATGGGATCGGCGACCCCCCAGATTAAAAACAATACCAAAACCACGC10.684931506849315No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
CTCTTCCGATCTGGTACCAACAGGTCCGAAAAACGAGCGTCGTGTAGGGA10.684931506849315No Hit
GAGTTACACTCTTTCCTCAGCAACTCCAGTCCTTGCATCATGAGATCGGA10.684931506849315No Hit
CGTATACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTTTAACTTAATTCTCACAATATCCCTATGAAGTAGATAAGTATATTCAT10.684931506849315No Hit
GGCCTTAGCGTTATTGAGCAAGCGCTGGCGGGTTTCATCCACCTCACTGC10.684931506849315No Hit
GGTGGGGAAGAAGCAAGAAAACAGGCACTAATTTCATTGTCACTGTCTCT10.684931506849315No Hit
CGGCGACACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT10.684931506849315Illumina Single End PCR Primer 1 (97% over 45bp)
GATGAGGAGATGATGGTCAAAGAGTACAAAGTTTCAGTTGTAGGATAGAT10.684931506849315No Hit
AACTTGTGCTCCTAATCACTATGATATATTGCCACATGGTTGAAGAAATT10.684931506849315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.684931506849315No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGTACGGCTGTCTCTTATACACA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.684931506849315Clontech SMART CDS Primer II A (100% over 24bp)
GCCCCGGAGCCGCCAAGGGTTTGAATGGTTGCTACGCGCTGTTGTTTCAG10.684931506849315No Hit
GTTCTTTTGTGTCAGAGTTCTTTTGCTTAGTATATTGCTGGAGAGGTTCC10.684931506849315No Hit
GTACATGGGATACGCGACCACGAGATCTACACTCTTTCCCTACACGACGC10.684931506849315Illumina Single End PCR Primer 1 (100% over 30bp)
ATCTGAAATTGTTCTCACAAAGGTCACCAGTGACTTTCCAATTGCAAATC10.684931506849315No Hit
TCTTAGTTGGTGGAGCGATTTGTGGGGTTTTTTTGGGTTACGATCGAGTC10.684931506849315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)