FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44.081632653061225Clontech Universal Primer Mix Long (96% over 26bp)
AATATAGGTCATATGAATAGTCACACTCGGTTTAGCTTCTGTTGAGCATT33.061224489795918No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA22.0408163265306123Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT22.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCCACTGACTCCCTGCTGCCCATGGACAGTCGGTCCCCGGCCGGGGG22.0408163265306123No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22.0408163265306123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGTAGATGACTGAGCTGGGATCTGAGAGGAGGCTCTCCTGACGGCCAC22.0408163265306123No Hit
CCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGGGAAA22.0408163265306123No Hit
AGCACCGCGTCGCCCGGAAGCATCGGCACCGATCCATAGGCTTCGCCGCC22.0408163265306123No Hit
AAAAACATTACACTTATACACATAAACAAACACAACACAAACGAACAAAT22.0408163265306123No Hit
GCCACACCGTCACCCACCGCTTTGCTGGCGAATGCTTCGTCAGGAACCTG22.0408163265306123No Hit
GCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGA22.0408163265306123No Hit
GGTCGGTGTCGACGCGGAAGAAGGTGCGGACGACCCAGTTGGCCGGGGCG11.0204081632653061No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG11.0204081632653061No Hit
AATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAACATGC11.0204081632653061No Hit
ACCTTGAGCAAGGCCTCTGAAAAACATCCCCAGACTAAAAGGATCTGTTT11.0204081632653061No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC11.0204081632653061No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTA11.0204081632653061No Hit
CTAGAAAAAACACAAAAACTAAAAGAAACAATACATATCAATCTTACACT11.0204081632653061No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.0204081632653061Clontech Universal Primer Mix Long (100% over 23bp)
CCTCTCTCGGTGGTCTGTCCGCCCAGCGCACGTCACCATGGGCAAAAGGT11.0204081632653061No Hit
GGTGGGTGTGGGTGACTACATTGGCATCTATGCCAGCATCAAGACAGACA11.0204081632653061No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGGAGTTTTTACC11.0204081632653061No Hit
ATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGT11.0204081632653061No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTGAGACGTTGTCGATAGCTGCTTAATCAATTTTAGTTCGGGCGCTT11.0204081632653061No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGTTGAAAACACTTCAACCC11.0204081632653061No Hit
CAGTGGTATCAACGCAGAGTACATGGGAATGATACGGCGAACACTCTTTC11.0204081632653061Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ACCCTGCCGTCCACGTAGGACTTGGTGGCGGCGTGATTATCACCCGACGG11.0204081632653061No Hit
GTAAGTGGAATGCCGCTGTAACAAATACCTAAAAAGGTGAAAGTGGCTTT11.0204081632653061No Hit
GAGTAGAATTGGTCTCCAAGAAGGATATATAGTTATTTCTAAGGCACTTT11.0204081632653061No Hit
TCATTGAATGCAATCACATGGAATCATCACAGAATGGAATCGTACGGAAT11.0204081632653061No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCCTAGCACATCAATGGGTAAAAATATGTACAGAATATTGTCAACCA11.0204081632653061No Hit
GGGTGTAGCCGGTATCGCCGCCGAAGGCCGGGCTGTCCGGCGTCCCGGTG11.0204081632653061No Hit
ACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAAAGGCAAGCCCTCCAGAGAGCGTTATGTGACCCACAACCGAGCAC11.0204081632653061No Hit
GTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGAC11.0204081632653061No Hit
GAGTACATGGGATACGCCTTACCTCTTCCCTACCGGAGCTGCCGATACCA11.0204081632653061No Hit
GTGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA11.0204081632653061Clontech Universal Primer Mix Long (95% over 21bp)
GGTCCTGAAAAACGAGCGTCGTGTAGGGAAAGATGTAGATCTCGGTGTCG11.0204081632653061No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.0204081632653061Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0204081632653061Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTGGGCTGGCACAGGGGAGCATGACTTCAACTCAGGAGGGGGCAGA11.0204081632653061No Hit
GTTTCAAAACTGCTCTGTCAATAGAAAGGTTCAACTCTGTTAGCTGCGTG11.0204081632653061No Hit
CTTAAGTTAATGAGTAGATCGGAAGAGCACACGTCTGCCCATGTACTCTG11.0204081632653061Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAACTTCAGGGTAGCGATCATTTGACAGTTCATCGTCCCTTTTCTGAACG11.0204081632653061No Hit
ATTCATTTCCATCCGATGATGATTCCATTCGATTCCGTTCAATGATTATT11.0204081632653061No Hit
CTCCCAATCTCTCTGGGGCTCCTCAGCCCGATCCCTCTGTAGCTCTCTTT11.0204081632653061No Hit
CCAGAGGTCTGTTGTGATGTCATCGCCTGTCGCTGCACCCGCTCCCGCGC11.0204081632653061No Hit
GCCAGGATGTCGTCGATGTGCTCGATCCCCACGCTCAGGCGCACGGTCCC11.0204081632653061No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT11.0204081632653061Clontech Universal Primer Mix Long (95% over 21bp)
ACCTTGAGCAAGGCCTCAGAAAAACATCCCCAGACTAAAATGATCTGTTT11.0204081632653061No Hit
GCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCC11.0204081632653061No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTA11.0204081632653061No Hit
ATGTTTTACTTTGCGATGAAGCTACAAGTGCATTAGACCCGCAAACAACG11.0204081632653061No Hit
GTCTCAGAGCTCATCTGGGTTCTAGAGCTGGGCTTGGTCTCGGAGCCCAT11.0204081632653061No Hit
ATCTCGTGTCGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGT11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
GACTTCACTGCAATCTTCACCTCCCGGGTTCGAGCGATTCTCCCACCTCA11.0204081632653061No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCACAGC11.0204081632653061No Hit
GGCTTGTCTTGAACTCCTGACCTCAGGTGATCCGCCCGCCTCAGCCTCCC11.0204081632653061No Hit
CCAAAAGCAATGGCAACAAAAGCCAAAATTGACAAATGGGATCTAATTAA11.0204081632653061No Hit
GAAGACGGCATACGAGATCTTGCTTCGTGAGTGGAGTTCAGACGTGCTGT11.0204081632653061Illumina PCR Primer Index 1 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTGTTTTGATACCACTGCTTCCC11.0204081632653061No Hit
GTACTCTTGCGTTGATACCACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCAAGAGCGTCGTGTAGGGAAGAGTGTAGACTCTGTCTCTTATACACAT11.0204081632653061No Hit
CTTCTTCGCCGTGACCGTGGCCGCCATGGACCAGCTCAACCTGAACATCC11.0204081632653061No Hit
GTATCAACGCAGAGTACATGGGAGCAGTCGTATCAACGCAGAGTACATGG11.0204081632653061No Hit
GGCTCGATCTGCGAGGTCGTGGATCCGGACGATCCCGACGACCGGGTCCT11.0204081632653061No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCGCACCGCC11.0204081632653061Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT11.0204081632653061Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCAAGTAGTTGGTTACAGGCTCATACCACCACGGCCGGCTAACTTTT11.0204081632653061No Hit
AACGCAGAGTACATGGGTGACGCACACCGAGTCTACACTCTTTCCCTACA11.0204081632653061No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAGGGTTCCCATT11.0204081632653061No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTTATACCACTTCTGTC11.0204081632653061Clontech SMART CDS Primer II A (95% over 22bp)
CTTCCGATCTATCCCTTTTTTGGGCCGGTTGGTACCAAAAAAGGGATAGA11.0204081632653061No Hit
CCACCTTGGCCTGCTCGAAGAGCGGAGCCACCAGGCCGTAGGCGATGCCG11.0204081632653061No Hit
GGGTGTAGCCGGTATCGCCGCCGAAGTCCGGGCTGACCGGCGTCCCGGTG11.0204081632653061No Hit
ACGCTCTTCCGATCTGTCCCAGGTCTGTCGATACCAGAACAGATCGGAAG11.0204081632653061No Hit
GCGGTGATACGCGTAATAGAGGACGTCGATTCCGAGCAGCAGGATCACCC11.0204081632653061No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAGTACTATT11.0204081632653061Clontech Universal Primer Mix Long (95% over 24bp)

[FAIL]Adapter Content

Adapter graph