FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCGGGGT35.454545454545454No Hit
ACACCAGCCAGAGGGGAATCACTCATCCCAGTGGTTGGAACTGGAGTTTC23.6363636363636362No Hit
GTATCAACGCAGAGTACATGGGACCTCCCTTCCCTACACGACGCTCTTCC23.6363636363636362Illumina Single End PCR Primer 1 (95% over 23bp)
TCCCGCCAATCATGGCGTAGGCCATCGGCCCCCAGAACACCTCGCGGGCA23.6363636363636362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGACGCCCACG23.6363636363636362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTTCGATTCGCGTTGCCGAACAGGCGCGCTACCAAGGCCACCAGAACTG23.6363636363636362No Hit
GAGCATGCTCACTTTTTTATATTAATTTTTACAGTATTTGTAAGAATAAA23.6363636363636362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCGAACCGAG11.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT11.8181818181818181No Hit
TGCGCGGGGAGCAGGCCGGAGAGGCTGCGGCCCCGGCCTCCGGGCGCCGC11.8181818181818181No Hit
GTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATCCTTGAGG11.8181818181818181No Hit
GAAAAGAGGGAGGTGGAATTGTTTTTTTTTTTTTTTTTAACGGGAGATAA11.8181818181818181No Hit
GTCATGACCATCGATGTTCAGGCGTCCGGTAGCCACGTCGATGGCGGGGC11.8181818181818181No Hit
TCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATTATAGCAAAGTGATGAGAGTCATTTTGAATTACGTTTAAATTGA11.8181818181818181No Hit
GAGTACATGGGATTACGGGACCACGAGATCTACACTCTTTCCCTACACGA11.8181818181818181Illumina Single End PCR Primer 1 (96% over 28bp)
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG11.8181818181818181No Hit
GAAGCAGTGGTATCAACCCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.8181818181818181Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGTCGTACCCGCAACCGGCCAGCCACGCCCTGGCGCTGACCTGCCAGGC11.8181818181818181No Hit
GTCATAGTGCCTGTCTGTGTTAGAATTGTTCCAGTGTCGCCAGGTAATCC11.8181818181818181No Hit
CAATAAACAAAGACTACAGTTGATACGAACACACACATATAAACAAAAAA11.8181818181818181No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8181818181818181No Hit
GTACATGGGCGTCTACATCTTCCCTACACGACGCTCTTGCCTCAGATTCT11.8181818181818181No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTACTCTGCCTG11.8181818181818181Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTAAACACAG11.8181818181818181No Hit
TGCGCGGGAAGCAGGCCGGAGAGGCTGCGGCCCCGGCCTCCGGGCGCCGC11.8181818181818181No Hit
ACCGCGTACCAATGCCGATGATGAGGTCGGCCTGGGCCGCCAGCGCATTC11.8181818181818181No Hit
GTCTACATCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCTGT11.8181818181818181Illumina Single End PCR Primer 1 (96% over 30bp)
GGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
CAATCACAGTGTAGCTAGGTTCCAAATCCCATATACGAGCGGGTAGCAGA11.8181818181818181No Hit
CCTGGTGGTCGCAACCCGGCCAGGCACGACGCTCTCCGAAGCAGCCCTAT11.8181818181818181No Hit
CACATCGATAATTTGGCCCTGCTGGTGGTGTTGACGGTGATTGGGTTTAT11.8181818181818181No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGT11.8181818181818181No Hit
CTACGTGGGAGCACCCTGACAGATGGCACTGCTATCCCCTCTCTGTCACT11.8181818181818181No Hit
GAGTACATGGGTGTACGCGCCACGAGTCAACACTCATTCCGGACTGGAGT11.8181818181818181No Hit
GGGCAGGGCTTTGCCTGAGGAGCCATCAAGGGATGGGCCCATGCTGATCT11.8181818181818181No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATCTGTCGGTAC11.8181818181818181Illumina Single End PCR Primer 1 (96% over 33bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
TGATTCAGGAGACAGAAACACCATGAGTATGTATGCACCTTCAAAATACA11.8181818181818181No Hit
CGCCTGGCCTATTTATTTTAAAATTTTAATTAATTTTTTTTTTGTTTTTA11.8181818181818181No Hit
GAAAAGAGGGAGGTGGAATTGTTTTTTTTTTTTTTTTTTTAAAGTGTGAT11.8181818181818181No Hit
ACGCAGAGTACATGGGATGACGGCGACCACCGAGATCTACATCTTTCCCT11.8181818181818181Illumina Single End PCR Primer 1 (95% over 22bp)
CCACAGAACCGCGACGGCGATCGCGACAATCCCCCACAGCCGCTCGGGAC11.8181818181818181No Hit
CTTCAAAGCCACATCATCGCGGTCAAAGTAGTAAGACATGGACAGGTAAA11.8181818181818181No Hit
GCATGAATATGTATGATGGAATTTCTTCCCAGCAATAGACTTCCAAACCA11.8181818181818181No Hit
GAAACTATGAAACTACTAGAAGAAAACATTGGAAAAACTCTCTAAGACAT11.8181818181818181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)