FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCC35.454545454545454No Hit
CCACAGGACAGTGAACGTTTCGTCTCTCCCAGCGAGACTCTCCCGCGGGC23.6363636363636362No Hit
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTTGTTGGTAC23.6363636363636362Illumina Single End PCR Primer 1 (100% over 25bp)
TCTTCCTTCTGCCCGCCCTCAAAAGGCAAAGAACCACTGCGGGCTGATGG23.6363636363636362No Hit
GAGTCACCTTTGTTGAGGTGAGCTGCACTTCATGGGGTTGGGAGAGGAAT23.6363636363636362No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
ATGGCAGACGCCCACGAGATTCACTCCTACACGACGCTCTTCCGATCTCT23.6363636363636362Illumina Single End Sequencing Primer (95% over 24bp)
GTACTGGAAGAGGTCCAGCGTGGCCATGCGGTCCGGTGTGACCATCAGCA23.6363636363636362No Hit
CCAACCACCGGCGCCGGCCGATTCTGCTGACCGCGGCGGCGGCGAGCCTG23.6363636363636362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GCGCGGGTCCGGTGACCTCGAGCAGACGGGTGAGCAGGGTCTGCATCGGG11.8181818181818181No Hit
CTGGAAACTCAGCAACAGGGACCCCAGCAACCACGACGAATATTGCCTCT11.8181818181818181No Hit
ACCCAGTACTCCCCATTGTGCTGTCAGCTGGGAAGAAGCCACGTAACCAC11.8181818181818181No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA11.8181818181818181No Hit
CCAGCCCCGCCATCGACGTGGCTACCGGACGCCTGAACATCGATGGTCAT11.8181818181818181No Hit
TATAAGTGCATCTCTTGTTGGACATAGGCAAAATAACTTGGCAAACTTAG11.8181818181818181No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA11.8181818181818181Illumina Single End PCR Primer 1 (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTTAAAATGAG11.8181818181818181No Hit
CGTCCACCTCGCAGGTGCGCCAGAACTACCACCAGGACTCAGAGGCCGCC11.8181818181818181No Hit
AGCTGCGGGACTTCGCATCTGCTACCGGCATCCCGGTCGCCGACACCCAG11.8181818181818181No Hit
GTGCCCAGACCAATGTCTTAGAGAGTTTTTCCAATGTTTTCTTCTAGTAG11.8181818181818181No Hit
ATCGGCGGTCGCGACCGCGTCCGGCGTGCGCCGCGCCGCCCAGAGCGGGC11.8181818181818181No Hit
CTGTTGGTGGGAGTGTAAATTAGTTCAACCATTGTGGAAGACAGTGTGGC11.8181818181818181No Hit
ACCGAGATCTACATCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGT11.8181818181818181Illumina Single End PCR Primer 1 (100% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC11.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACAAAAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGAA11.8181818181818181No Hit
CTCTTCCGATCTCTGGTACCGACAGATCTGAGGCAAGAGCGTCGTGTAGG11.8181818181818181No Hit
GCGATATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
CTGATGTATCGATTTTGCCTCATGTATTTTGAAGGTGCATACATACTCAT11.8181818181818181No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGTCTACATCTTCC11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCAGCCAGGCTTTTATAGGAGAGGAGGTCCTGGAAGTGAAGACAGAG11.8181818181818181No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGGGTTGAT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.8181818181818181Clontech Universal Primer Mix Long (95% over 21bp)
GTGCCTTGTCGGGTGAAGATGTTGTGTTTTGGGAAACGAAGCACCTTACC11.8181818181818181No Hit
ACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGC11.8181818181818181No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTAAAATTGG11.8181818181818181No Hit
CACCTGTAATCCCAGCACTTTGGGAGGCTGAGAGGGAAGATCACTTGAGG11.8181818181818181No Hit
ACATGGGTGCGAACCGAGTTAATCTTTCCCTACACGGAGCTGCCGATACC11.8181818181818181No Hit
GGTCTGCATTGTTAACTTGTACTCAGAAGCCTCTGGCAATCGTCTCTGGT11.8181818181818181No Hit
ACAGGCAACCGGCTGGTGCGGCTGGCGGGCCTCGTGCTGGCGCTGGCGAT11.8181818181818181No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAATAAAAAAAAAAAAAGTAAACT11.8181818181818181Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCAG11.8181818181818181Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAC11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
TCTATGAACGTGTTCATCCGTCATCTCTCTTATGCGCCACAAATCGGGCC11.8181818181818181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)